FLCN (NM_144997) Human Tagged ORF Clone

CAT#: RC220396

FLCN (Myc-DDK-tagged)-Human folliculin (FLCN), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY408135) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC220396 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified FLCN protein (Cat# TP320396). The protein was produced from HEK293T cells transfected with FLCN cDNA clone (Cat# RC220396) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: FLCN
• Synonyms: BHD; DENND8B; FLCL
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC220396 representing NM_144997
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATGCCATCGTGGCTCTCTGCCACTTCTGCGAGCTCCACGGCCCCCGCACTCTCTTCTGCACGGAGG
  TGCTGCACGCCCCACTTCCTCAAGGGGATGGGAATGAGGACAGTCCTGGCCAGGGTGAGCAGGCGGAAGA
  AGAGGAAGGTGGCATTCAGATGAACAGTCGGATGCGTGCGCACAGCCCCGCAGAGGGGGCCAGCGTCGAG
  TCCAGCAGCCCGGGGCCCAAAAAGTCGGACATGTGCGAGGGCTGCCGGTCACTTGCTGCAGGGCACCCGG
  GATATATCAGCCATGATAAAGAGACCTCCATTAAATACGTCAGCCACCAGCACCCCAGCCACCCCCAGCT
  CTTCAGCATTGTCCGCCAGGCCTGTGTCCGGAGCCTGAGCTGTGAGGTCTGCCCTGGCCGTGAAGGCCCC
  ATCTTCTTCGGAGATGAGCAGCACGGCTTTGTGTTCAGCCACACCTTCTTCATCAAGGACAGCCTGGCCA
  GGGGCTTCCAGCGCTGGTACAGCATCATCACCATCATGATGGACCGGATCTACCTCATCAACTCCTGGCC
  CTTCCTGCTGGGGAAGGTCCGGGGAATCATCGATGAGCTCCAGGGCAAGGCGCTCAAGGTGTTTGAGGCA
  GAGCAGTTTGGATGCCCACAGCGTGCTCAGAGGATGAACACAGCCTTCACGCCATTCCTACACCAGAGGA
  ACGGCAACGCCGCCCGCTCGCTGACATCGCTGACAAGTGATGACAACCTGTGGGCGTGCCTGCACACCTC
  CTTTGCCTGGCTCCTGAAGGCGTGTGGCAGCCGGCTGACCGAGAAGCTCCTGGAAGGTGCTCCGACCGAG
  GATACCTTGGTCCAGATGGAGAAGCTCGCTGATTTAGAAGAGGAATCAGAAAGCTGGGACAACTCTGAGG
  CTGAAGAGGAGGAGAAAGCCCCTGTGTTGCCAGAGAGTACAGAAGGGCGGGAGCTGACCCAGGGCCCGGC
  AGAGTCCTCCTCTCTCTCAGGCTGTGGGAGCTGGCAGCCCCGGAAGCTGCCAGTCTTCAAGTCCCTCCGG
  CACATGAGGCAGGTCCTGGGTGCCCCTTCTTTCCGCATGCTGGCCTGGCACGTTCTCATGGGGAACCAGG
  TGATCTGGAAAAGCAGAGACGTGGACCTCGTCCAGTCAGCTTTTGAAGTACTTCGGACCATGCTTCCCGT
  GGGCTGCGTCCGCATCATCCCATACAGCAGCCAGTACGAGGAGGCCTATCGGTGCAACTTCCTGGGGCTC
  AGCCCGCACGTGCAGATCCCCCCCCACGTGCTCTCCTCAGAGTTTGCTGTCATCGTGGAGGTCCACGCAG
  CCGCACGTTCCACCCTCCACCCTGTGGGGTGTGAGGATGACCAGTCTCTCAGCAAGTACGAGTTTGTGGT
  GACCAGTGGGAGCCCTGTAGCTGCAGACCGAGTGGGCCCCACCATCCTGAATAAGATTGAAGCGGCTCTG
  ACCAACCAGAACCTGTCTGTGGATGTGGTGGACCAGTGCCTCGTCTGCCTCAAGGAGGAGTGGATGAACA
  AAGTGAAGGTGCTTTTTAAGTTCACCAAGGTGGACAGTCGACCCAAAGAGGACACACAGAAGCTGCTGAG
  CATCCTGGGTGCGTCCGAGGAGGACAATGTCAAGCTGCTGAAGTTCTGGATGACTGGCCTGAGCAAGACC
  TACAAGTCACACCTCATGTCCACGGTCCGCAGCCCCACAGCCTCGGAGTCTCGGAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC220396 representing NM_144997
  Red=Cloning site Green=Tags(s)
  
  MNAIVALCHFCELHGPRTLFCTEVLHAPLPQGDGNEDSPGQGEQAEEEEGGIQMNSRMRAHSPAEGASVE
  SSSPGPKKSDMCEGCRSLAAGHPGYISHDKETSIKYVSHQHPSHPQLFSIVRQACVRSLSCEVCPGREGP
  IFFGDEQHGFVFSHTFFIKDSLARGFQRWYSIITIMMDRIYLINSWPFLLGKVRGIIDELQGKALKVFEA
  EQFGCPQRAQRMNTAFTPFLHQRNGNAARSLTSLTSDDNLWACLHTSFAWLLKACGSRLTEKLLEGAPTE
  DTLVQMEKLADLEEESESWDNSEAEEEEKAPVLPESTEGRELTQGPAESSSLSGCGSWQPRKLPVFKSLR
  HMRQVLGAPSFRMLAWHVLMGNQVIWKSRDVDLVQSAFEVLRTMLPVGCVRIIPYSSQYEEAYRCNFLGL
  SPHVQIPPHVLSSEFAVIVEVHAAARSTLHPVGCEDDQSLSKYEFVVTSGSPVAADRVGPTILNKIEAAL
  TNQNLSVDVVDQCLVCLKEEWMNKVKVLFKFTKVDSRPKEDTQKLLSILGASEEDNVKLLKFWMTGLSKT
  YKSHLMSTVRSPTASESRN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_144997
• ORF Size: 1737 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_144997.2, NM_144997.3, NM_144997.4, NM_144997.5, NP_659434.2
• RefSeq Size: 3717 bp
• RefSeq ORF: 1740 bp
• Locus ID: 201163
• Protein Families: Druggable Genome
• Protein Pathways: Renal cell carcinoma
• MW: 64.3 kDa
• Gene Summary: This gene is located within the Smith-Magenis syndrome region on chromosome 17. Mutations in this gene are associated with Birt-Hogg-Dube syndrome, which is characterized by fibrofolliculomas, renal tumors, lung cysts, and pneumothorax. Alternative splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]