TPST2 (NM_003595) Human Tagged ORF Clone

CAT#: RC220456

TPST2 (Myc-DDK-tagged)-Human tyrosylprotein sulfotransferase 2 (TPST2), transcript variant 2

Product Images

Western blot validation of overexpression lysate (Cat# LY418564) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC220456 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TPST2
• Synonyms: TANGO13B; TPST-2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC220456 representing NM_003595
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGCCTGTCGGTGCGGAGGGTGCTGCTGGCAGCCGGCTGCGCCCTGGTCCTGGTGCTGGCGGTTCAGC
  TGGGACAGCAGGTGCTAGAGTGCCGGGCGGTGCTGGCGGGCCTGCGGAGCCCCCGGGGGGCCATGCGGCC
  TGAGCAGGAGGAGCTGGTGATGGTGGGCACCAACCACGTGGAATACCGCTATGGCAAGGCCATGCCGCTC
  ATCTTCGTGGGTGGCGTGCCTCGCAGTGGCACCACGTTGATGCGCGCCATGCTGGACGCCCACCCCGAGG
  TGCGCTGCGGCGAGGAGACCCGCATCATCCCGCGCGTGCTGGCCATGCGCCAGGCCTGGTCCAAGTCTGG
  CCGTGAGAAGCTGCGGCTGGATGAGGCGGGGGTGACGGATGAGGTGCTGGACGCCGCCATGCAGGCCTTC
  ATCCTGGAGGTGATTGCCAAGCACGGAGAGCCGGCCCGCGTGCTCTGCAACAAGGACCCATTTACGCTCA
  AGTCCTCGGTCTACCTGTCGCGCCTGTTCCCCAACTCCAAGTTCCTGCTGATGGTGCGGGACGGCCGGGC
  CTCCGTGCACTCCATGATCACGCGCAAAGTCACCATTGCGGGCTTTGACCTCAGCAGCTACCGTGACTGC
  CTCACCAAGTGGAACAAGGCCATCGAGGTGATGTACGCCCAGTGCATGGAGGTAGGCAAGGAGAAGTGCC
  TGCCTGTGTACTACGAGCAGCTGGTGCTGCACCCCAGGCGCTCACTCAAGCTCATCCTCGACTTCCTCGG
  CATCGCCTGGAGCGACGCTGTCCTCCACCATGAAGACCTCATTGGCAAGCCCGGTGGTGTCTCCCTGTCC
  AAGATCGAGCGGTCCACGGACCAGGTCATCAAGCCTGTTAACCTGGAAGCGCTCTCCAAGTGGACTGGCC
  ACATCCCTGGGGATGTGGTGCGGGACATGGCCCAGATCGCCCCCATGCTGGCTCAGCTCGGCTATGACCC
  TTATGCAAACCCCCCCAACTATGGCAACCCTGACCCCTTCGTCATCAACAACACACAGCGGGTCTTGAAA
  GGGGACTATAAAACACCAGCCAATCTGAAAGGATATTTTCAGGTGAACCAGAACAGCACCTCCTCCCACT
  TAGGAAGCTCG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC220456 representing NM_003595
  Red=Cloning site Green=Tags(s)
  
  MRLSVRRVLLAAGCALVLVLAVQLGQQVLECRAVLAGLRSPRGAMRPEQEELVMVGTNHVEYRYGKAMPL
  IFVGGVPRSGTTLMRAMLDAHPEVRCGEETRIIPRVLAMRQAWSKSGREKLRLDEAGVTDEVLDAAMQAF
  ILEVIAKHGEPARVLCNKDPFTLKSSVYLSRLFPNSKFLLMVRDGRASVHSMITRKVTIAGFDLSSYRDC
  LTKWNKAIEVMYAQCMEVGKEKCLPVYYEQLVLHPRRSLKLILDFLGIAWSDAVLHHEDLIGKPGGVSLS
  KIERSTDQVIKPVNLEALSKWTGHIPGDVVRDMAQIAPMLAQLGYDPYANPPNYGNPDPFVINNTQRVLK
  GDYKTPANLKGYFQVNQNSTSSHLGSS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003595
• ORF Size: 1131 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003595.1, NM_003595.2, NM_003595.3, NP_003586.3
• RefSeq Size: 1910 bp
• RefSeq ORF: 1134 bp
• Locus ID: 8459
• Domains: Sulfotransfer
• Protein Families: Transmembrane
• MW: 41.7 kDa
• Gene Summary: The protein encoded by this gene catalyzes the O-sulfation of tyrosine residues within acidic regions of proteins. The encoded protein is a type II integral membrane protein found in the Golgi body. Alternative splicing produces multiple transcript variants encoding distinct isoforms. [provided by RefSeq, May 2018]