BRCC45 (BRE) (NM_199191) Human Tagged ORF Clone

CAT#: RC220494

BRE (Myc-DDK-tagged)-Human brain and reproductive organ-expressed (TNFRSF1A modulator) (BRE), transcript variant 3

Product Images

Western blot validation of overexpression lysate (Cat# LY404687) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC220494 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: BABAM2
• Synonyms: BRCC4; BRCC45; BRE
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC220494 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCCCAGAAGTGGCCTTGAACCGAATATCTCCAATGCTCTCCCCTTTCATATCTAGCGTGGTCCGGA
  ATGGAAAAGTGGGACTGGATGCTACAAACTGTTTGAGGATAACTGACTTAAAATCTGGCTGCACATCATT
  GACTCCTGGGCCCAACTGTGACCGATTTAAACTGCACATACCATATGCTGGAGAGACATTAAAGTGGGAT
  ATCATTTTCAATGCCCAATACCCAGAACTGCCTCCCGATTTTATCTTTGGAGAAGATGCTGAATTCCTGC
  CAGACCCCTCAGCTTTGCAGAATCTTGCCTCCTGGAATCCTTCAAATCCTGAATGTCTCTTACTTGTGGT
  GAAGGAACTTGTGCAACAATATCACCAATTCCAATGTAGCCGCCTCCGGGAGAGCTCCCGCCTCATGTTT
  GAATACCAGACATTACTGGAGGAGCCACAGTATGGAGAGAACATGGAAATTTATGCTGGGAAAAAAAACA
  ACTGGACTGGTGAATTTTCAGCTCGTTTCCTTTTGAAGCTGCCCGTAGATTTCAGCAATATCCCCACATA
  CCTTCTCAAGGATGTAAATGAAGACCCTGGAGAAGATGTGGCCCTCCTCTCTGTTAGTTTTGAGGACACT
  GAAGCCACCCAGGTGTACCCCAAGCTGTACTTGTCACCTCGAATTGAGCATGCACTTGGAGGCTCCTCAG
  CTCTTCATATCCCAGCTTTTCCAGGAGGAGGATGTCTCATTGATTACGTTCCTCAAGTATGCCACCTGCT
  CACCAACAAGGTGCAGTACGTGATTCAAGGGTATCACAAAAGAAGAGAGTATATTGCTGCTTTTCTCAGT
  CACTTTGGCACAGGTGTCGTGGAATATGATGCAGAAGGCTTTACAAAACTCACTCTGCTGCTGATGTGGA
  AAGATTTTTGTTTTCTTGTACACATTGACCTGCCTCTGTTTTTCCCTCGAGACCAGCCAACTCTCACATT
  TCAGTCCGTTTATCACTTTACCAACAGTGGACAGCTTTACTCCCAGGCCCAAAAAAATTATCCGTACAGC
  CCCAGATGGGATGGAAATGAAATGGCCAAAAGAGCAAAGGCTTATTTCAAAACCTTTGTCCCTCAGTTCC
  AGGAGGCAGCATTTGCCAATGGAAAGCTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC220494 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSPEVALNRISPMLSPFISSVVRNGKVGLDATNCLRITDLKSGCTSLTPGPNCDRFKLHIPYAGETLKWD
  IIFNAQYPELPPDFIFGEDAEFLPDPSALQNLASWNPSNPECLLLVVKELVQQYHQFQCSRLRESSRLMF
  EYQTLLEEPQYGENMEIYAGKKNNWTGEFSARFLLKLPVDFSNIPTYLLKDVNEDPGEDVALLSVSFEDT
  EATQVYPKLYLSPRIEHALGGSSALHIPAFPGGGCLIDYVPQVCHLLTNKVQYVIQGYHKRREYIAAFLS
  HFGTGVVEYDAEGFTKLTLLLMWKDFCFLVHIDLPLFFPRDQPTLTFQSVYHFTNSGQLYSQAQKNYPYS
  PRWDGNEMAKRAKAYFKTFVPQFQEAAFANGKL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_199191
• ORF Size: 1149 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_199191.1, NM_199191.2, NP_954661.1
• RefSeq Size: 1804 bp
• RefSeq ORF: 1152 bp
• Locus ID: 9577
• Protein Families: Druggable Genome
• MW: 43.6 kDa
• Gene Summary: This gene encodes an anti-apoptotic, death receptor-associated protein that interacts with tumor necrosis factor-receptor-1. The encoded protein acts as an adapter in several protein complexes, including the BRCA1-A complex and the BRISC complex. The BRCA1-A complex possesses ubiquitinase activity and targets sites of double strand DNA breaks, while the BRISC complex exhibits deubiquitinase activity and is involved in mitotic spindle assembly. This gene is upregulated in several types of cancer. [provided by RefSeq, Jun 2016]