CTPS2 (NM_175859) Human Tagged ORF Clone

CAT#: RC220566

CTPS2 (Myc-DDK-tagged)-Human CTP synthase II (CTPS2), transcript variant 2

Product Images

Western blot validation of overexpression lysate (Cat# LY428462) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC226565 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified CTPS2 protein (Cat# TP320566). The protein was produced from HEK293T cells transfected with CTPS2 cDNA clone (Cat# RC220566) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CTPS2
• Synonyms: DKFZp686C17207; FLJ43358; MGC32997
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC220566 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGTACATCCTGGTCACGGGTGGGGTCATCTCAGGCATTGGTAAAGGGATCATTGCCAGCAGCATTG
  GAACGATTCTAAAATCATGTGGACTCCGAGTTACTGCCATAAAAATCGACCCCTATATTAACATCGATGC
  TGGCACTTTTTCACCTTATGAACACGGTGAAGTCTTCGTCTTAAATGATGGTGGAGAAGTTGATTTAGAC
  CTTGGAAATTATGAAAGATTTTTGGATATTAATCTTTATAAAGACAACAATATCACCACGGGGAAGATAT
  ATCAGCATGTGATCAATAAAGAGAGGCGTGGTGATTACCTGGGGAAAACAGTGCAAGTTGTCCCTCACAT
  TACTGATGCTGTCCAGGAGTGGGTTATGAATCAAGCCAAGGTGCCGGTGGATGGTAATAAGGAAGAGCCC
  CAAATATGCGTTATTGAGCTGGGAGGCACCATTGGAGACATCGAAGGAATGCCGTTTGTGGAGGCGTTTA
  GACAATTCCAGTTTAAGGCGAAAAGAGAGAATTTCTGTAATATCCACGTTAGCCTTGTCCCACAGCTCAG
  TGCTACCGGAGAACAAAAAACCAAACCCACCCAAAACAGCGTCCGCGCACTGAGGGGTTTAGGCCTGTCT
  CCAGATCTGATTGTCTGCCGAAGTTCAACGCCCATTGAGATGGCCGTGAAGGAGAAGATTTCTATGTTTT
  GTCACGTGAACCCTGAACAGGTCATATGTATCCATGATGTTTCTTCCACATACCGAGTTCCTGTGCTTTT
  AGAGGAACAAAGCATTGTGAAATATTTTAAGGAGAGATTGCACCTGCCCATCGGTGATTCTGCAAGTAAT
  TTGCTTTTTAAGTGGAGAAATATGGCTGACAGGTATGAAAGGTTACAGAAAATATGCTCCATAGCCCTGG
  TTGGCAAATACACCAAGCTCAGAGACTGCTACGCCTCTGTGTTCAAAGCCCTGGAACACTCAGCCCTGGC
  CATCAACCACAAGTTGAATCTGATGTACATAGACTCCATTGATCTGGAGAAGATCACTGAAACCGAGGAC
  CCTGTGAAATTTCATGAAGCTTGGCAGAAGCTATGCAAAGCTGATGGTATTCTTGTGCCTGGAGGCTTTG
  GAATCAGAGGAACATTGGGAAAACTCCAGGCGATTTCTTGGGCAAGGACAAAGAAGATTCCTTTTCTGGG
  AGTTTGTCTTGGGATGCAACTAGCAGTGATAGAGTTTGCAAGAAACTGCCTTAACTTGAAAGATGCTGAT
  TCCACAGAGTTTAGGCCAAATGCCCCAGTTCCTCTGGTGATTGATATGCCCGAGCACAACCCTGGCAATT
  TGGGAGGAACAATGAGACTGGGAATAAGAAGAACTGTTTTCAAAACTGAAAATTCAATATTAAGGAAACT
  TTATGGTGATGTTCCTTTTATAGAAGAAAGACACAGACATCGGTTCGAGGTAAACCCTAACCTGATCAAA
  CAATTTGAGCAGAATGACTTAAGTTTTGTAGGTCAGGATGTTGATGGAGACAGGATGGAAATCATTGAAC
  TGGCAAATCATCCTTATTTTGTTGGTGTCCAGTTCCATCCTGAGTTTTCTTCTAGGCCGATGAAGCCTTC
  CCCTCCGTATCTGGGGCTGTTACTTGCAGCAACTGGGAACCTGAATGCCTACTTGCAACAGGGTTGCAAA
  CTGTCTTCCAGTGATAGATACAGTGATGCCAGTGATGACAGCTTTTCAGAGCCAAGGATAGCTGAGTTGG
  AAATAAGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC220566 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MKYILVTGGVISGIGKGIIASSIGTILKSCGLRVTAIKIDPYINIDAGTFSPYEHGEVFVLNDGGEVDLD
  LGNYERFLDINLYKDNNITTGKIYQHVINKERRGDYLGKTVQVVPHITDAVQEWVMNQAKVPVDGNKEEP
  QICVIELGGTIGDIEGMPFVEAFRQFQFKAKRENFCNIHVSLVPQLSATGEQKTKPTQNSVRALRGLGLS
  PDLIVCRSSTPIEMAVKEKISMFCHVNPEQVICIHDVSSTYRVPVLLEEQSIVKYFKERLHLPIGDSASN
  LLFKWRNMADRYERLQKICSIALVGKYTKLRDCYASVFKALEHSALAINHKLNLMYIDSIDLEKITETED
  PVKFHEAWQKLCKADGILVPGGFGIRGTLGKLQAISWARTKKIPFLGVCLGMQLAVIEFARNCLNLKDAD
  STEFRPNAPVPLVIDMPEHNPGNLGGTMRLGIRRTVFKTENSILRKLYGDVPFIEERHRHRFEVNPNLIK
  QFEQNDLSFVGQDVDGDRMEIIELANHPYFVGVQFHPEFSSRPMKPSPPYLGLLLAATGNLNAYLQQGCK
  LSSSDRYSDASDDSFSEPRIAELEIS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_175859
• ORF Size: 1758 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_175859.1, NM_175859.2, NP_787055.1
• RefSeq Size: 3973 bp
• RefSeq ORF: 1761 bp
• Locus ID: 56474
• Protein Pathways: Metabolic pathways, Pyrimidine metabolism
• MW: 65.7 kDa
• Gene Summary: The protein encoded by this gene catalyzes the formation of CTP from UTP with the concomitant deamination of glutamine to glutamate. This protein is the rate-limiting enzyme in the synthesis of cytosine nucleotides, which play an important role in various metabolic processes and provide the precursors necessary for the synthesis of RNA and DNA. Cancer cells that exhibit increased cell proliferation also exhibit an increased activity of this encoded protein. Thus, this protein is an attractive target for selective chemotherapy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013]