Leucyl cystinyl aminopeptidase (LNPEP) (NM_005575) Human Tagged ORF Clone

CAT#: RC220692

LNPEP (Myc-DDK-tagged)-Human leucyl/cystinyl aminopeptidase (LNPEP), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY401710) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC220692 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified LNPEP protein (Cat# TP320692). The protein was produced from HEK293T cells transfected with LNPEP cDNA clone (Cat# RC220692) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: LNPEP
• Synonyms: CAP; IRAP; P-LAP; PLAP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC220692 representing NM_005575
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCCCTTCACCAATGATCGGCTTCAGCTCCCCAGGAATATGATTGAAAACAGCATGTTTGAGGAAG
  AACCAGATGTGGTGGATTTAGCCAAAGAGCCTTGTTTACATCCTCTAGAGCCTGATGAGGTGGAATATGA
  GCCCCGGGGTTCCCGACTGCTGGTGCGGGGTCTTGGTGAGCATGAGATGGAGGAGGATGAAGAGGATTAT
  GAGTCATCAGCAAAGCTGCTGGGCATGTCCTTCATGAATAGAAGCTCAGGCCTTCGGAACAGTGCAACTG
  GTTACAGGCAGAGCCCAGATGGGGCTTGTTCAGTACCCTCTGCAAGGACCATGGTGGTCTGTGCTTTTGT
  CATCGTGGTTGCTGTTTCTGTAATCATGGTGATTTACTTACTGCCCAGATGTACCTTTACCAAAGAAGGC
  TGCCATAAAAAAAACCAGTCAATTGGACTAATTCAGCCATTTGCAACAAATGGGAAATTGTTTCCATGGG
  CACAGATCAGGCTTCCCACTGCCGTTGTGCCACTACGCTATGAACTCAGCCTACACCCGAACCTAACCTC
  GATGACATTCAGGGGTTCTGTGACAATTTCAGTTCAGGCTCTTCAGGTCACATGGAATATCATTCTTCAT
  AGCACAGGTCATAATATTTCAAGAGTGACCTTTATGTCAGCAGTTTCAAGCCAAGAAAAACAAGCTGAGA
  TCCTGGAATATGCATATCATGGACAGATCGCCATTGTTGCCCCCGAAGCCCTTCTAGCAGGGCACAATTA
  TACGTTGAAGATAGAGTACTCGGCAAATATATCTAGTTCTTATTATGGGTTTTATGGCTTCTCCTACACA
  GATGAAAGTAATGAGAAAAAGTACTTTGCAGCAACTCAGTTTGAACCCCTGGCAGCAAGATCTGCTTTTC
  CTTGTTTTGATGAACCAGCATTTAAAGCCACTTTTATCATCAAGATCATAAGGGATGAGCAATACACCGC
  TTTATCAAATATGCCTAAGAAGTCATCAGTCGTTCTAGATGATGGACTTGTTCAGGATGAGTTTTCTGAG
  AGTGTGAAGATGAGCACTTACTTGGTTGCTTTCATTGTGGGAGAGATGAAGAACCTGAGTCAGGACGTAA
  ATGGAACCCTGGTTTCTATATATGCTGTACCAGAAAAGATTGGTCAAGTTCATTATGCCTTGGAAACAAC
  TGTGAAGCTTCTTGAGTTTTTTCAAAACTACTTTGAAATTCAGTACCCACTTAAGAAATTGGATTTGGTG
  GCTATTCCTGACTTTGAAGCAGGAGCAATGGAAAATTGGGGTTTGCTCACCTTCCGAGAGGAGACACTTC
  TGTATGACAGTAACACTTCTTCAATGGCGGATAGAAAGCTGGTGACTAAAATCATTGCTCATGAGCTGGC
  CCACCAGTGGTTTGGCAATCTGGTAACAATGAAGTGGTGGAATGACCTATGGCTAAATGAAGGTTTTGCC
  ACTTTCATGGAGTATTTCTCTTTGGAAAAAATATTCAAAGAGCTTTCTAGTTATGAAGATTTCTTAGATG
  CTCGATTTAAAACCATGAAGAAAGATTCCTTAAATTCATCTCATCCAATATCATCATCTGTTCAGTCTTC
  AGAACAAATTGAAGAAATGTTTGATTCTCTTTCCTATTTTAAGGGATCTTCTCTCTTGTTGATGTTGAAA
  ACTTACCTTAGTGAAGATGTGTTTCAACATGCTGTTGTCCTTTACCTGCATAATCACAGCTATGCATCTA
  TTCAAAGTGATGATCTGTGGGATAGTTTTAATGAGGTCACAAACCAAACACTAGATGTAAAGAGAATGAT
  GAAAACCTGGACCCTGCAGAAAGGATTTCCTTTAGTGACTGTTCAAAAGAAAGGAAAGGAACTTTTTATA
  CAACAAGAGAGATTCTTTTTAAATATGAAGCCTGAAATTCAGCCTTCAGATACAAGCTACCTGTGGCATA
  TTCCACTATCCTATGTCACTGAAGGAAGAAATTATTCAAAATATCAATCGGTATCATTACTGGATAAGAA
  ATCAGGTGTCATCAATCTTACAGAAGAAGTGCTGTGGGTCAAAGTGAATATAAACATGAATGGTTATTAT
  ATTGTACACTATGCAGATGATGATTGGGAAGCACTAATCCATCAGTTGAAAATAAATCCTTATGTTCTGA
  GTGACAAAGACCGAGCCAACCTTATCAACAACATCTTTGAACTTGCAGGCCTAGGCAAGGTACCTCTCAA
  GAGGGCCTTTGATTTGATTAATTATCTTGGAAATGAGAACCATACTGCACCCATCACCGAAGCCCTGTTT
  CAGACAGACCTCATCTATAACCTCCTTGAAAAACTGGGATACATGGATCTGGCCTCAAGACTGGTGACTA
  GGGTATTTAAATTACTTCAAAACCAAATTCAACAACAAACTTGGACTGATGAGGGCACTCCATCTATGCG
  AGAGCTTCGGTCAGCCCTGCTAGAGTTTGCTTGCACCCACAACCTGGGGAACTGCTCTACTACTGCCATG
  AAACTGTTTGATGACTGGATGGCATCCAATGGAACTCAAAGCCTACCTACTGATGTCATGACAACTGTGT
  TCAAAGTTGGAGCAAAAACTGACAAAGGCTGGTCATTCCTTTTGGGCAAATACATTTCTATAGGCTCTGA
  AGCAGAGAAGAACAAAATACTAGAAGCACTTGCCAGCTCAGAGGATGTGCGGAAGCTTTACTGGTTAATG
  AAAAGTAGCCTGAATGGAGATAACTTCCGAACACAGAAGCTGTCTTTTATCATTAGAACAGTGGGTCGAC
  ATTTTCCTGGACACTTACTGGCATGGGATTTTGTCAAAGAGAACTGGAATAAGCTTGTACAGAAGTTCCC
  TCTGGGGTCCTATACCATACAAAATATTGTTGCTGGATCAACTTACCTGTTTTCAACAAAGACACATTTA
  TCTGAGGTTCAGGCATTCTTTGAAAATCAGTCAGAGGCAACCTTCCGGCTTCGTTGTGTCCAGGAGGCTT
  TGGAAGTCATTCAGTTGAATATCCAGTGGATGGAGAAGAACCTCAAAAGTCTCACATGGTGGCTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC220692 representing NM_005575
  Red=Cloning site Green=Tags(s)
  
  MEPFTNDRLQLPRNMIENSMFEEEPDVVDLAKEPCLHPLEPDEVEYEPRGSRLLVRGLGEHEMEEDEEDY
  ESSAKLLGMSFMNRSSGLRNSATGYRQSPDGACSVPSARTMVVCAFVIVVAVSVIMVIYLLPRCTFTKEG
  CHKKNQSIGLIQPFATNGKLFPWAQIRLPTAVVPLRYELSLHPNLTSMTFRGSVTISVQALQVTWNIILH
  STGHNISRVTFMSAVSSQEKQAEILEYAYHGQIAIVAPEALLAGHNYTLKIEYSANISSSYYGFYGFSYT
  DESNEKKYFAATQFEPLAARSAFPCFDEPAFKATFIIKIIRDEQYTALSNMPKKSSVVLDDGLVQDEFSE
  SVKMSTYLVAFIVGEMKNLSQDVNGTLVSIYAVPEKIGQVHYALETTVKLLEFFQNYFEIQYPLKKLDLV
  AIPDFEAGAMENWGLLTFREETLLYDSNTSSMADRKLVTKIIAHELAHQWFGNLVTMKWWNDLWLNEGFA
  TFMEYFSLEKIFKELSSYEDFLDARFKTMKKDSLNSSHPISSSVQSSEQIEEMFDSLSYFKGSSLLLMLK
  TYLSEDVFQHAVVLYLHNHSYASIQSDDLWDSFNEVTNQTLDVKRMMKTWTLQKGFPLVTVQKKGKELFI
  QQERFFLNMKPEIQPSDTSYLWHIPLSYVTEGRNYSKYQSVSLLDKKSGVINLTEEVLWVKVNINMNGYY
  IVHYADDDWEALIHQLKINPYVLSDKDRANLINNIFELAGLGKVPLKRAFDLINYLGNENHTAPITEALF
  QTDLIYNLLEKLGYMDLASRLVTRVFKLLQNQIQQQTWTDEGTPSMRELRSALLEFACTHNLGNCSTTAM
  KLFDDWMASNGTQSLPTDVMTTVFKVGAKTDKGWSFLLGKYISIGSEAEKNKILEALASSEDVRKLYWLM
  KSSLNGDNFRTQKLSFIIRTVGRHFPGHLLAWDFVKENWNKLVQKFPLGSYTIQNIVAGSTYLFSTKTHL
  SEVQAFFENQSEATFRLRCVQEALEVIQLNIQWMEKNLKSLTWWL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005575
• ORF Size: 3075 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005575.1, NM_005575.2, NP_005566.2
• RefSeq Size: 4470 bp
• RefSeq ORF: 3078 bp
• Locus ID: 4012
• Cytogenetics: 5q15
• Domains: Peptidase_M1
• Protein Families: Druggable Genome, Protease, Secreted Protein, Transmembrane
• Protein Pathways: Renin-angiotensin system
• MW: 117.2 kDa
• Gene Summary: 'This gene encodes a zinc-dependent aminopeptidase that cleaves vasopressin, oxytocin, lys-bradykinin, met-enkephalin, dynorphin A and other peptide hormones. The protein can be secreted in maternal serum, reside in intracellular vesicles with the insulin-responsive glucose transporter GLUT4, or form a type II integral membrane glycoprotein. The protein catalyzes the final step in the conversion of angiotensinogen to angiotensin IV (AT4) and is also a receptor for AT4. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]'