Leucyl cystinyl aminopeptidase (LNPEP) (NM_005575) Human Untagged Clone

CAT#: SC116663

LNPEP (untagged)-Human leucyl/cystinyl aminopeptidase (LNPEP), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: LNPEP
• Synonyms: CAP; IRAP; P-LAP; PLAP
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC116663 sequence for NM_005575 edited (data generated by NextGen Sequencing)
  ATGGAGCCCTTCACCAATGATCGGCTTCAGCTCCCCAGGAATATGATTGAAAACAGCATG
  TTTGAGGAAGAACCAGATGTGGTGGATTTAGCCAAAGAGCCTTGTTTACATCCTCTAGAG
  CCTGATGAGGTGGAATATGAGCCCCGGGGTTCCCGACTGCTGGTGCGGGGTCTTGGTGAG
  CATGAGATGGAGGAGGATGAAGAGGATTATGAGTCATCAGCAAAGCTGCTGGGCATGTCC
  TTCATGAATAGAAGCTCAGGCCTTCGGAACAGTGCAACTGGTTACAGGCAGAGCCCAGAT
  GGGGCTTGTTCAGTACCCTCTGCAAGGACCATGGTGGTCTGTGCTTTTGTCATCGTGGTT
  GCTGTTTCTGTAATCATGGTGATTTACTTACTGCCCAGATGTACCTTTACCAAAGAAGGC
  TGCCATAAAAAAAACCAGTCAATTGGACTAATTCAGCCATTTGCAACAAATGGGAAATTG
  TTTCCATGGGCACAGATCAGGCTTCCCACTGCCGTTGTGCCACTACGCTATGAACTCAGC
  CTACACCCGAACCTAACCTCGATGACATTCAGGGGTTCTGTGACAATTTCAGTTCAGGCT
  CTTCAGGTCACATGGAATATCATTCTTCATAGCACAGGTCATAATATTTCAAGAGTGACC
  TTTATGTCAGCAGTTTCAAGCCAAGAAAAACAAGCTGAGATCCTGGAATATGCATATCAT
  GGACAGATCGCCATTGTTGCCCCCGAAGCCCTTCTAGCAGGGCACAATTATACGTTGAAG
  ATAGAGTACTCGGCAAATATATCTAGTTCTTATTATGGGTTTTATGGCTTCTCCTACACA
  GATGAAAGTAATGAGAAAAAGTACTTTGCAGCAACTCAGTTTGAACCCCTGGCAGCAAGA
  TCTGCTTTTCCTTGTTTTGATGAACCAGCATTTAAAGCCACTTTTATCATCAAGATCATA
  AGGGATGAGCAATACACCGCTTTATCAAATATGCCTAAGAAGTCATCAGTCGTTCTAGAT
  GATGGACTTGTTCAGGATGAGTTTTCTGAGAGTGTGAAGATGAGCACTTACTTGGTTGCT
  TTCATTGTGGGAGAGATGAAGAACCTGAGTCAGGACGTAAATGGAACCCTGGTTTCTATA
  TATGCTGTACCAGAAAAGATTGGTCAAGTTCATTATGCCTTGGAAACAACTGTGAAGCTT
  CTTGAGTTTTTTCAAAACTACTTTGAAATTCAGTACCCACTTAAGAAATTGGATTTGGTG
  GCTATTCCTGACTTTGAAGCAGGAGCAATGGAAAATTGGGGTTTGCTCACCTTCCGAGAG
  GAGACACTTCTGTATGACAGTAACACTTCTTCAATGGCGGATAGAAAGCTGGTGACTAAA
  ATCATTGCTCATGAGCTGGCCCACCAGTGGTTTGGCAATCTGGTAACAATGAAGTGGTGG
  AATGACCTATGGCTAAATGAAGGTTTTGCCACTTTCATGGAGTATTTCTCTTTGGAAAAA
  ATATTCAAAGAGCTTTCTAGTTATGAAGATTTCTTAGATGCTCGATTTAAAACCATGAAG
  AAAGATTCCTTAAATTCATCTCATCCAATATCATCATCTGTTCAGTCTTCAGAACAAATT
  GAAGAAATGTTTGATTCTCTTTCCTATTTTAAGGGATCTTCTCTCTTGTTGATGTTGAAA
  ACTTACCTTAGTGAAGATGTGTTTCAACATGCTGTTGTCCTTTACCTGCATAATCACAGC
  TATGCATCTATTCAAAGTGATGATCTGTGGGATAGTTTTAATGAGGTCACAAACCAAACA
  CTAGATGTAAAGAGAATGATGAAAACCTGGACCCTGCAGAAAGGATTTCCTTTAGTGACT
  GTTCAAAAGAAAGGAAAGGAACTTTTTATACAACAAGAGAGATTCTTTTTAAATATGAAG
  CCTGAAATTCAGCCTTCAGATACAAGCTACCTGTGGCATATTCCACTATCCTATGTCACT
  GAAGGAAGAAATTATTCAAAATATCAATCGGTATCATTACTGGATAAGAAATCAGGTGTC
  ATCAATCTTACAGAAGAAGTGCTGTGGGTCAAAGTGAATATAAACATGAATGGTTATTAT
  ATTGTACACTATGCAGATGATGATTGGGAAGCACTAATCCATCAGTTGAAAATAAATCCT
  TATGTTCTGAGTGACAAAGACCGAGCCAACCTTATCAACAACATCTTTGAACTTGCAGGC
  CTAGGCAAGGTACCTCTCAAGAGGGCCTTTGATTTGATTAATTATCTTGGAAATGAGAAC
  CATACTGCACCCATCACCGAAGCCCTGTTTCAGACAGACCTCATCTATAACCTCCTTGAA
  AAACTGGGATACATGGATCTGGCCTCAAGACTGGTGACTAGGGTATTTAAATTACTTCAA
  AACCAAATTCAACAACAAACTTGGACTGATGAGGGCACTCCATCTATGCGAGAGCTTCGG
  TCAGCCCTGCTAGAGTTTGCTTGCACCCACAACCTGGGGAACTGCTCTACTACTGCCATG
  AAACTGTTTGATGACTGGATGGCATCCAATGGAACTCAAAGCCTACCTACTGATGTCATG
  ACAACTGTGTTCAAAGTTGGAGCAAAAACTGACAAAGGCTGGTCATTCCTTTTGGGCAAA
  TACATTTCTATAGGCTCTGAAGCAGAGAAGAACAAAATACTAGAAGCACTTGCCAGCTCA
  GAGGATGTGCGGAAGCTTTACTGGTTAATGAAAAGTAGCCTGAATGGAGATAACTTCCGA
  ACACAGAAGCTGTCTTTTATCATTAGAACAGTGGGTCGACATTTTCCTGGACACTTACTG
  GCATGGGATTTTGTCAAAGAGAACTGGAATAAGCTTGTACAGAAGTTCCCTCTGGGGTCC
  TATACCATACAAAATATTGTTGCTGGATCAACTTACCTGTTTTCAACAAAGACACATTTA
  TCTGAGGTTCAGGCATTCTTTGAAAATCAGTCAGAGGCAACCTTCCGGCTTCGTTGTGTC
  CAGGAGGCTTTGGAAGTCATTCAGTTGAATATCCAGTGGATGGAGAAGAACCTCAAAAGT
  CTCACATGGTGGCTGTAG
  
  Clone variation with respect to NM_005575.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005575 unedited
  NNNCCGTTCAGATTTGTAAACGACTCACTATAGGCGGCCGCGAATTCGGCACCAGGCCTC
  AGCTCTCGGATAGGAAGCTCGGGCGCTCCGGCTGTAAGGAGCCGCGGCGGGGGGAAAATG
  GAGCCCTTCACCAATGATCGGCTTCAGCTCCCCAGGAATATGATTGAAAACAGCATGTTT
  GAGGAAGAACCAGATGTGGTGGATTTAGCCAAAGAGCCTTGTTTACATCCTCTAGAGCCT
  GATGAGGTGGAATATGAGCCCCGGGGTTCCCGACTGCTGGTGCGGGGTCTTGGTGAGCAT
  GAGATGGAGGAGGATGAAGAGGATTATGAGTCATCAGCAAAGCTGCTGGGCATGTCCTTC
  ATGAATAGAAGCTCAGGCCTTCGGAACAGTGCAACTGGTTACAGGCAGAGCCCAGATGGG
  GCTTGTTCAGTACCCTCTGCAAGGACCATGGTGGTCTGTGCTTTTGTCATCGTGGTTGCT
  GTTTCTGTAATCATGGTGATTTACTTACTGCCCAGATGTACCTTTACCAAAGAAGGCTGC
  CATAAAAAAAACCAGTCAATTGGACTAATTCAGCCATTTGCAACAAATGGGAAATTGTTT
  CCATGGGCACAGATCAGGCTTCCCACTGCCGTTGTGCCACTACGCTATGAACTCAGCCTA
  CACCCGAACCTAACCTCGATGACATTCANNGGGTTCTGNTGACATTTCAGTTCAGGCTCT
  TCAGGTCACATGGAATATCATTCTTCATAGCACAGGTCATAATATTTCAAGAGTGACCTT
  TATGTCAGCAGTTTCAAGCCAAGAAAAACAGCTGAGATCCTGGAATATGCATATCATGGA
  CAGATCGCCATTGTTGCCCCCCGAACCCTTCTAGCAGGNCACAATTATACGTTTGAGATA
  GAGTACTCGGCAATATA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005575 unedited
  NGTTCTGTTTTTTTTTATTTTTTTTACAGTTCTTACAGTGCATGCTTTATTGTCTTCGTT
  ACCATATTCTTCAGAGCTAACTTTGTACCACATTCCAATGCCATCTATCAATAAGAAGCA
  ACAGATGGCTGACAAACCAGCGGCCTGAAGTGATGGCGGCTCTTAGGGTAGAACTTTCCT
  TGTTTTACTGTGTCAGTAACTTTCTGGATACTGAGGAATAGAACAGAATTCCAAAACAGG
  GTTTTTGTCCCCATCTGTGTATCTTCAAAGTAGAAAAATTACTCATTGTTTTTGTAGATG
  GTTGGCATTTAGGTGAATATAATTTTGTAATAAATAACTACATACTGCCCAAAGACACTT
  CAGAAAAGATGGATGAAAAATACAGTCAGGCCCTGAGCTAACTAGAACTCCAAAGAGTGC
  AAACAACTTGGCAGCGATCCTGGCTTTACCTTGGCTTTTGCAAAAGCGGCAGAGCCCAAG
  CTTACAAGCTCTCTGAATGGGCAACAAAATGAGGTGCGGTTGTGCATGCTACAGCCACCA
  TGTGAGACTTTTGAGGTTCTTCTCCATCCACTGGATATTCAACTGAATGACTTCCAAAGC
  CTCCTGGACACAACGAAGCCGGAAGGGTGCCTCTGACTGATTTTTCAAGGATGCCTGGAC
  CTCAGATAAATGTGTCTTTGTTGGAAACAGGTAAGTTGATCCCAGCACATAATTTTGTAT
  GGTATAGGACCCCAGAAGGGACTTCTGTACAAGCTTATTCCAGTTTCTCTTTGACAAATC
  CCATGCCAGTNAGTTGTCCAGAAAATGTTGACCCACTGGTTCTATGATAAAAGACAGCTT
  CTGGTTC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005575
• Insert Size: 4000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005575.2, NP_005566.2
• RefSeq Size: 4470 bp
• RefSeq ORF: 3078 bp
• Locus ID: 4012
• Cytogenetics: 5q15
• Domains: Peptidase_M1
• Protein Families: Druggable Genome, Protease, Secreted Protein, Transmembrane
• Protein Pathways: Renin-angiotensin system
• Gene Summary: 'This gene encodes a zinc-dependent aminopeptidase that cleaves vasopressin, oxytocin, lys-bradykinin, met-enkephalin, dynorphin A and other peptide hormones. The protein can be secreted in maternal serum, reside in intracellular vesicles with the insulin-responsive glucose transporter GLUT4, or form a type II integral membrane glycoprotein. The protein catalyzes the final step in the conversion of angiotensinogen to angiotensin IV (AT4) and is also a receptor for AT4. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).