NPB (NM_148896) Human Tagged ORF Clone
CAT#: RC220796
- TrueORF®
NPB (Myc-DDK-tagged)-Human neuropeptide B (NPB)
"NM_148896" in other vectors (4)
Product Images
![](https://cdn.origene.com/img/defaults-img-expression-plasmids.jpg)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | NPB |
Synonyms | L7; PPL7; PPNPB |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC220796 representing NM_148896
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCCGGTCCGCGACACTGGCGGCCGCCGCCCTGGCGCTGTGCCTGCTGCTGGCGCCGCCTGGCCTCG CGTGGTACAAGCCAGCGGCGGGGCACAGCTCCTACTCGGTGGGCCGCGCCGCGGGGCTGCTGTCCGGCCT CCGCAGGTCCCCGTACGCGCGGCGCTCCCAGCCCTACAGAGGGGCGGAACCCCCGGGCGGGGCCGGCGCC TCCCCGGAGCTGCAACTGCACCCCAGGCTGCGGAGCCTCGCTGTGTGCGTCCAGGACGTCGCCCCAAACC TGCAGAGGTGCGAGCGGCTCCCCGACGGCCGCGGGACCTACCAGTGCAAGGCGAACGTCTTCCTGTCCCT GCGCGCAGCCGACTGCCTCGCCGCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC220796 representing NM_148896
Red=Cloning site Green=Tags(s) MARSATLAAAALALCLLLAPPGLAWYKPAAGHSSYSVGRAAGLLSGLRRSPYARRSQPYRGAEPPGGAGA SPELQLHPRLRSLAVCVQDVAPNLQRCERLPDGRGTYQCKANVFLSLRAADCLAA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_148896 |
ORF Size | 375 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_148896.1, NM_148896.2, NM_148896.3, NM_148896.4, NP_683694.1 |
RefSeq Size | 430 bp |
RefSeq ORF | 378 bp |
Locus ID | 256933 |
Protein Families | Secreted Protein, Transmembrane |
MW | 12.9 kDa |
Gene Summary | This gene encodes a member of the neuropeptide B/W family of proteins and preproprotein that is proteolytically processed to generate multiple protein products. The encoded products include neuropeptide B-23 and a C-terminally extended form, neuropeptide B-29, which are characterized by an N-terminal brominated tryptophan amino acid. Both of the encoded peptides bind with higher affinity to neuropeptide B/W (NPB/W) receptor 1 compared to the related NPB/W receptor 2. These peptides may regulate feeding, pain perception, and stress in rodents. [provided by RefSeq, Jul 2015] |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC306326 | NPB (untagged)-Human neuropeptide B (NPB) |
USD 420.00 |
|
RG220796 | NPB (GFP-tagged) - Human neuropeptide B (NPB) |
USD 460.00 |
|
RC220796L3 | Lenti ORF clone of Human neuropeptide B (NPB), Myc-DDK-tagged |
USD 620.00 |
|
RC220796L4 | Lenti ORF clone of Human neuropeptide B (NPB), mGFP tagged |
USD 620.00 |
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