PCDHA6 (NM_031849) Human Tagged ORF Clone

CAT#: RC220820

PCDHA6 (Myc-DDK-tagged)-Human protocadherin alpha 6 (PCDHA6), transcript variant 3

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PCDHA6
• Synonyms: CNR2; CNRN2; CNRS2; CRNR2; PCDH-ALPHA6
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC220820 representing NM_031849
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGTTTACCCCGGAGGATAGATTGGGAAAGCAATGTCTGCTCCTCCCGCTTCTGCTCCTCGCAGCCT
  GGAAGGTGGGGAGCGGCCAGCTCCACTACTCCGTACCCGAGGAGGCCAAACACGGCACCTTCGTGGGCCG
  GATCGCGCAGGACCTGGGGCTGGAGCTGGCGGAGCTGGTGCCGCGCCTGTTCAGGATGGCCTCCAAAGAC
  CGCGAGGACCTTCTGGAGGTAAATCTGCAGAATGGCATTTTGTTTGTGAATTCTCGGATCGACCGCGAGG
  AGCTGTGCGGGCGGAGCGCGGAGTGCAGCATCCACCTGGAGGTGATCGTGGACAGGCCGCTGCAGGTTTT
  CCATGTGGACGTGGAGGTGAGGGACATTAACGACAACCCGCCCTTGTTCCCGGTAGAGGAACAAAGAGTG
  CTGATTTACGAATCTAGGCTGCCAGATTCTGTGTTTCCACTGGAGGGCGCGTCCGATGCAGATGTTGGCT
  CAAATTCCATCTTAACCTATAAACTCAGTTCTAGCGAATACTTCGGGCTAGATGTGAAAATAAACAGTGA
  TGACAATAAACAAATTGGGCTCTTATTAAAGAAATCCTTGGACAGAGAGGAAGCTCCTGCACACAACTTA
  TTCCTGACAGCCACAGATGGGGGCAAACCTGAGCTCACAGGCACTGTTCAGCTGCTGGTCACAGTGCTGG
  ATGTGAATGATAATGCTCCCACTTTCGAACAGTCTGAATACGAAGTAAGAATATTCGAAAATGCAGACAA
  CGGAACAACAGTTATCAGACTGAATGCTTCTGATCGGGATGAAGGAGCGAATGGGGCAATTTCATATTCT
  TTTAATAGCCTTGTTGCAGCCATGGTTATTGACCACTTTAGCATAGATCGAAATACGGGAGAAATAGTGA
  TTCGGGGTAATTTGGATTTTGAACAAGAAAACTTATACAAAATCCTCATTGACGCCACGGACAAAGGCCA
  TCCTCCCATGGCGGGTCATTGCACCGTTTTAGTGAGAATTTTGGATAAAAATGATAACGTCCCTGAGATA
  GCACTGACTTCCTTATCCTTGCCTGTACGTGAAGACGCTCAATTTGGTACTGTCATCGCCCTAATTAGCG
  TGAACGACCTCGATTCAGGTGCCAACGGGCAGGTGAACTGCTCGCTGACGCCTCACGTCCCTTTCAAGCT
  GGTGTCCACCTTCAAGAATTACTACTCGTTGGTGCTGGACAGTGCCCTGGACCGCGAGAGCGTGTCGGCC
  TATGAGTTGGTGGTAACCGCGCGGGACGGGGGCTCGCCTTCGCTGTGGGCCACCGCCAGCTTGTCTGTGG
  AGGTGGCCGACATGAATGACAATGCTCCGGCGTTCGCGCAGCCCGAGTACACAGTGTTCGTGAAGGAGAA
  CAACCCGCCGGGCTGCCACATCTTCACGGTGTCTGCGCGAGACGCGGACGCGCAGGAGAACGCGCTGGTG
  TCCTACTCGCTGGTGGAGCGGCGGGTGGGCGAGCGCGCGTTGTCGAGCTACATTTCGGTGCACGCGGAGA
  GCGGCAAGGTGTACGCGCTGCAGCCGCTGGACCACGAGGAGCTAGAGCTGCTGCAGTTTCAGCCACGACA
  GCCCAACCCTGACTGGCGTTACTCTGCCTCCCTGAGAGCAGGCATGCACAGCTCTGTGCACCTAGAGGAG
  GCTGGCATTCTACGGGCTGGTCCAGGAGGGCCTGATCAGCAGTGGCCAACAGTATCCAGTGCAACACCAG
  AACCAGAGGCAGGAGAAGTGTCCCCTCCAGTCGGTGCGGGTGTCAACAGCAACAGCTGGACCTTTAAATA
  CGGACCAGGCAACCCCAAACAATCCGGTCCCGGTGAGTTGCCCGACAAATTCATTATCCCAGGATCTCCT
  GCAATCATCTCCATCCGGCAGGAGCCTACTAACAGCCAAATTGACAAAAGTGACTTCATAACCTTCGGCA
  AAAAGGAGGAGACCAAGAAAAAGAAGAAAAAGAAGAAGGGTAACAAGACCCAGGAGAAAAAAGAGAAAGG
  GAACAGCACGACTGACAACAGTGACCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC220820 representing NM_031849
  Red=Cloning site Green=Tags(s)
  
  MVFTPEDRLGKQCLLLPLLLLAAWKVGSGQLHYSVPEEAKHGTFVGRIAQDLGLELAELVPRLFRMASKD
  REDLLEVNLQNGILFVNSRIDREELCGRSAECSIHLEVIVDRPLQVFHVDVEVRDINDNPPLFPVEEQRV
  LIYESRLPDSVFPLEGASDADVGSNSILTYKLSSSEYFGLDVKINSDDNKQIGLLLKKSLDREEAPAHNL
  FLTATDGGKPELTGTVQLLVTVLDVNDNAPTFEQSEYEVRIFENADNGTTVIRLNASDRDEGANGAISYS
  FNSLVAAMVIDHFSIDRNTGEIVIRGNLDFEQENLYKILIDATDKGHPPMAGHCTVLVRILDKNDNVPEI
  ALTSLSLPVREDAQFGTVIALISVNDLDSGANGQVNCSLTPHVPFKLVSTFKNYYSLVLDSALDRESVSA
  YELVVTARDGGSPSLWATASLSVEVADMNDNAPAFAQPEYTVFVKENNPPGCHIFTVSARDADAQENALV
  SYSLVERRVGERALSSYISVHAESGKVYALQPLDHEELELLQFQPRQPNPDWRYSASLRAGMHSSVHLEE
  AGILRAGPGGPDQQWPTVSSATPEPEAGEVSPPVGAGVNSNSWTFKYGPGNPKQSGPGELPDKFIIPGSP
  AIISIRQEPTNSQIDKSDFITFGKKEETKKKKKKKKGNKTQEKKEKGNSTTDNSDQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_031849
• ORF Size: 2058 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_031849.1, NM_031849.2, NP_114037.1
• RefSeq Size: 4495
• RefSeq ORF: 2061
• Locus ID: 56142
• Domains: CA
• Protein Families: Secreted Protein, Transmembrane
• MW: 72 kDa
• Gene Summary: This gene is a member of the protocadherin alpha gene cluster, one of three related gene clusters tandemly linked on chromosome five that demonstrate an unusual genomic organization similar to that of B-cell and T-cell receptor gene clusters. The alpha gene cluster is composed of 15 cadherin superfamily genes related to the mouse CNR genes and consists of 13 highly similar and 2 more distantly related coding sequences. The tandem array of 15 N-terminal exons, or variable exons, are followed by downstream C-terminal exons, or constant exons, which are shared by all genes in the cluster. The large, uninterrupted N-terminal exons each encode six cadherin ectodomains while the C-terminal exons encode the cytoplasmic domain. These neural cadherin-like cell adhesion proteins are integral plasma membrane proteins that most likely play a critical role in the establishment and function of specific cell-cell connections in the brain. Alternative splicing has been observed and additional variants have been suggested but their full-length nature has yet to be determined. [provided by RefSeq, Jul 2008]