KCNG4 (NM_172347) Human Tagged ORF Clone

CAT#: RC220851

KCNG4 (Myc-DDK-tagged)-Human potassium voltage-gated channel, subfamily G, member 4 (KCNG4)

Product Images

Western blot validation of overexpression lysate (Cat# LY406715) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC220851 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: KCNG4
• Synonyms: KV6.3; KV6.4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC220851 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCCATGCCTTCCAGAGACGGGGGCCTGCATCCCAGACACCACCACTATGGTTCCCACAGCCCTTGGA
  GTCAGCTCCTGTCCAGCCCCATGGAGACGCCGTCCATCAAGGGCCTTTACTACCGGAGGGTGCGGAAGGT
  GGGTGCCCTGGACGCCTCCCCAGTGGACCTGAAGAAGGAGATCCTGATCAACGTGGGGGGCAGGAGGTAT
  CTCCTCCCCTGGAGCACACTGGACCGGTTCCCGCTGAGCCGCCTGAGCAAACTCAGGCTCTGTCGGAGCT
  ACGAGGAGATCGTGCAGCTCTGCGATGATTACGACGAGGACAGCCAGGAGTTCTTCTTCGACAGGAGCCC
  CAGCGCCTTCGGGGTGATCGTGAGCTTCCTGGCGGCCGGGAAGCTGGTGCTTCTGCAGGAGATGTGCGCG
  CTGTCCTTCCAGGAGGAGCTGGCCTACTGGGGCATCGAGGAGGCCCACCTGGAGAGGTGCTGCCTGCGGA
  AGCTGCTGAGGAAGCTGGAGGAGCTGGAGGAGCTGGCCAAGCTGCACAGGGAGGACGTACTGAGGCAGCA
  GAGGGAGACCCGCCGCCCCGCCTCGCACTCCTCGCGCTGGGGCCTGTGCATGAACCGGCTGCGCGAGATG
  GTGGAAAACCCGCAGTCCGGGCTGCCCGGGAAGGTCTTCGCTTGCCTCTCCATCCTCTTCGTGGCCACCA
  CAGCCGTCAGCCTGTGTGTCAGCACCATGCCCGACCTCAGGGCAGAGGAGGACCAGGGCGAATGCTCTCG
  GAAGTGCTACTATATTTTCATCGTGGAGACCATCTGCGTGGCCTGGTTCTCCCTGGAGTTCTGCCTGCGG
  TTTGTCCAGGCCCAAGACAAGTGTCAGTTCTTCCAGGGGCCCCTGAACATCATCGACATCCTGGCCATCT
  CCCCATACTACGTGTCGCTGGCGGTGTCTGAGGAGCCCCCGGAGGACGGCAAGAGGCCGAGCGGGAGCTC
  CTACCTGGAGAAGGTGGGGCTGGTCCTGCGTGTGCTGCGAGCGCTGCGCATCCTCTACGTGATGCGCCTG
  GCTCGCCACTCGCTGGGGCTGCAGACGCTGGGGCTCACCGTGCGCCGTTGCACACGTGAGTTCGGCCTGC
  TCCTTCTCTTCCTGGCCGTGGCCATCACCCTCTTCTCCCCTTTGGTCTACGTGGCCGAGAAGGAGTCCGG
  GCGGGTGCTGGAGTTCACCAGCATCCCCGCCTCCTATTGGTGGGCCATCATCTCCATGACAACGGTGGGC
  TACGGGGACATGGTGCCCCACAGTGTGCCAGGCCAGATGGTGGCCCTCAGCAGCATCCTGAGCGGGATCC
  TCATCATGGCCTTCCCGGCCACGTCTATCTTCCACACCTTCTCCCACTCCTACCTGGAGCTCAAGAAGGA
  GCAGGAGCAGCTTCAGGCCCGCCTCCGCCACCTCCAAAACACCGGTCCAGCCAGTGAATGTGAACTCCTG
  GACCCCCATGTGGCCAGTGAACATGAGCTCATGAACGATGTCAATGACCTAATCCTGGAGGGCCCAGCCT
  TGCCTATCATGCACATGT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC220851 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MPMPSRDGGLHPRHHHYGSHSPWSQLLSSPMETPSIKGLYYRRVRKVGALDASPVDLKKEILINVGGRRY
  LLPWSTLDRFPLSRLSKLRLCRSYEEIVQLCDDYDEDSQEFFFDRSPSAFGVIVSFLAAGKLVLLQEMCA
  LSFQEELAYWGIEEAHLERCCLRKLLRKLEELEELAKLHREDVLRQQRETRRPASHSSRWGLCMNRLREM
  VENPQSGLPGKVFACLSILFVATTAVSLCVSTMPDLRAEEDQGECSRKCYYIFIVETICVAWFSLEFCLR
  FVQAQDKCQFFQGPLNIIDILAISPYYVSLAVSEEPPEDGKRPSGSSYLEKVGLVLRVLRALRILYVMRL
  ARHSLGLQTLGLTVRRCTREFGLLLLFLAVAITLFSPLVYVAEKESGRVLEFTSIPASYWWAIISMTTVG
  YGDMVPHSVPGQMVALSSILSGILIMAFPATSIFHTFSHSYLELKKEQEQLQARLRHLQNTGPASECELL
  DPHVASEHELMNDVNDLILEGPALPIMHM
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_172347
• ORF Size: 1558 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_172347.1, NM_172347.2, NP_758857.1
• RefSeq Size: 2779 bp
• RefSeq ORF: 1560 bp
• Locus ID: 93107
• Protein Families: Druggable Genome, Ion Channels: Potassium, Transmembrane
• MW: 59 kDa
• Gene Summary: Voltage-gated potassium (Kv) channels represent the most complex class of voltage-gated ion channels from both functional and structural standpoints. Their diverse functions include regulating neurotransmitter release, heart rate, insulin secretion, neuronal excitability, epithelial electrolyte transport, smooth muscle contraction, and cell volume. This gene encodes a member of the potassium channel, voltage-gated, subfamily G. This member functions as a modulatory subunit. The gene has strong expression in brain. Multiple alternatively spliced variants have been found in normal and cancerous tissues. [provided by RefSeq, Jul 2008]