ADAM11 (NM_002390) Human Tagged ORF Clone

CAT#: RC220941

ADAM11 (Myc-DDK-tagged)-Human ADAM metallopeptidase domain 11 (ADAM11)

Product Images

Western blot validation of overexpression lysate (Cat# LY419357) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC220941 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified ADAM11 protein (Cat# TP320941). The protein was produced from HEK293T cells transfected with ADAM11 cDNA clone (Cat# RC220941) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ADAM11
• Synonyms: MDC
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC220941 representing NM_002390
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGCTGCTGCGGCGCTGGGCGTTCGCGGCTCTGCTGCTGTCGCTGCTCCCCACGCCCGGTCTTGGGA
  CCCAAGGTCCTGCTGGAGCTCTGCGATGGGGGGGCTTACCCCAGCTGGGAGGCCCAGGAGCCCCTGAGGT
  CACGGAACCCAGCCGTCTGGTTAGGGAGAGCTCCGGGGGAGAGGTCCGAAAGCAGCAGCTGGACACAAGG
  GTCCGCCAGGAGCCACCAGGGGGCCCGCCTGTCCATCTGGCCCAGGTGAGTTTCGTCATCCCAGCCTTCA
  ACTCAAACTTCACCCTGGACCTGGAGCTGAACCACCACCTCCTCTCCTCGCAATACGTGGAGCGCCACTT
  CAGCCGGGAGGGGACAACCCAGCACAGCACCGGGGCTGGAGACCACTGCTACTACCAGGGGAAGCTCCGG
  GGGAACCCGCACTCCTTCGCCGCCCTCTCCACCTGCCAGGGGCTGCATGGGGTCTTCTCTGATGGGAACT
  TGACTTACATCGTGGAGCCCCAAGAGGTGGCTGGACCTTGGGGAGCCCCTCAGGGACCCCTTCCCCACCT
  CATTTACCGGACCCCTCTCCTCCCAGATCCCCTCGGATGCAGGGAACCAGGCTGCCTGTTTGCTGTGCCT
  GCCCAGTCGGCTCCTCCAAACCGGCCGAGGCTGAGAAGGAAAAGGCAGGTCCGCCGGGGCCACCCTACAG
  TGCACAGTGAAACCAAGTATGTGGAGCTAATTGTGATCAACGACCACCAGCTGTTCGAGCAGATGCGACA
  GTCGGTGGTCCTCACCAGCAACTTTGCCAAGTCCGTGGTGAACCTGGCCGATGTGATATACAAGGAGCAG
  CTCAACACTCGCATCGTCCTGGTTGCCATGGAAACATGGGCAGATGGGGACAAGATCCAGGTGCAGGATG
  ACCTCCTGGAGACCCTGGCCCGGCTCATGGTCTACCGACGGGAGGGTCTGCCTGAGCCCAGTGATGCCAC
  CCACCTCTTCTCGGGCAGGACCTTCCAGAGCACGAGCAGCGGGGCAGCCTACGTGGGGGGCATATGCTCC
  CTGTCCCACGGCGGGGGTGTGAACGAGTACGGCAACATGGGGGCGATGGCCGTGACCCTTGCCCAGACGC
  TGGGACAGAACCTGGGCATGATGTGGAACAAACACCGGAGCTCGGCAGGGGACTGCAAGTGTCCAGACAT
  CTGGCTGGGCTGCATCATGGAGGACACTGGGTTCTACCTGCCCCGCAAGTTCTCGCGCTGTAGCATCGAC
  GAGTACAACCAGTTTCTGCAGGAGGGTGGTGGCAGCTGCCTCTTCAACAAGCCCCTCAAGCTCCTGGACC
  CCCCAGAGTGCGGGAACGGCTTCGTGGAGGCAGGGGAGGAGTGCGACTGCGGCTCGGTGCAGGAGTGCAG
  CCGCGCAGGTGGCAACTGCTGCAAGAAATGCACCCTGACTCACGACGCCATGTGCAGCGACGGGCTCTGC
  TGTCGCCGCTGCAAGTACGAACCACGGGGTGTGTCCTGCCGAGAGGCCGTGAACGAGTGCGACATCGCGG
  AGACCTGCACCGGGGACTCTAGCCAGTGCCCGCCTAACCTGCACAAGCTGGACGGTTACTACTGTGACCA
  TGAGCAGGGCCGCTGCTACGGAGGTCGCTGCAAAACCCGGGACCGGCAGTGCCAGGTTCTTTGGGGCCAT
  GCGGCTGCTGATCGCTTCTGCTACGAGAAGCTGAATGTGGAGGGGACGGAGCGTGGGAGCTGTGGGCGCA
  AGGGATCTGGCTGGGTCCAGTGCAGTAAGCAGGACGTGCTGTGTGGCTTCCTCCTCTGTGTCAACATCTC
  TGGAGCTCCTCGGCTAGGGGACCTGGTGGGAGACATCAGTAGTGTCACCTTCTACCACCAGGGCAAGGAG
  CTGGACTGCAGGGGAGGCCACGTGCAGCTGGCGGACGGCTCTGACCTGAGCTATGTGGAGGATGGCACAG
  CCTGCGGGCCTAACATGTTGTGCCTGGACCATCGCTGCCTGCCAGCTTCTGCCTTCAACTTCAGCACCTG
  CCCCGGCAGTGGGGAGCGCCGGATTTGCTCCCACCACGGGGTCTGCAGCAATGAAGGGAAGTGCATCTGT
  CAGCCAGACTGGACAGGCAAAGACTGCAGTATCCATAACCCCCTGCCCACGTCCCCACCCACGGGGGAGA
  CGGAGAGATATAAAGGTCCCAGCGGCACCAACATCATCATTGGCTCCATTGCTGGGGCTGTCCTGGTTGC
  AGCCATCGTCCTGGGCGGCACGGGCTGGGGATTTAAAAACATTCGCCGAGGAAGGTCCGGAGGGGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC220941 representing NM_002390
  Red=Cloning site Green=Tags(s)
  
  MRLLRRWAFAALLLSLLPTPGLGTQGPAGALRWGGLPQLGGPGAPEVTEPSRLVRESSGGEVRKQQLDTR
  VRQEPPGGPPVHLAQVSFVIPAFNSNFTLDLELNHHLLSSQYVERHFSREGTTQHSTGAGDHCYYQGKLR
  GNPHSFAALSTCQGLHGVFSDGNLTYIVEPQEVAGPWGAPQGPLPHLIYRTPLLPDPLGCREPGCLFAVP
  AQSAPPNRPRLRRKRQVRRGHPTVHSETKYVELIVINDHQLFEQMRQSVVLTSNFAKSVVNLADVIYKEQ
  LNTRIVLVAMETWADGDKIQVQDDLLETLARLMVYRREGLPEPSDATHLFSGRTFQSTSSGAAYVGGICS
  LSHGGGVNEYGNMGAMAVTLAQTLGQNLGMMWNKHRSSAGDCKCPDIWLGCIMEDTGFYLPRKFSRCSID
  EYNQFLQEGGGSCLFNKPLKLLDPPECGNGFVEAGEECDCGSVQECSRAGGNCCKKCTLTHDAMCSDGLC
  CRRCKYEPRGVSCREAVNECDIAETCTGDSSQCPPNLHKLDGYYCDHEQGRCYGGRCKTRDRQCQVLWGH
  AAADRFCYEKLNVEGTERGSCGRKGSGWVQCSKQDVLCGFLLCVNISGAPRLGDLVGDISSVTFYHQGKE
  LDCRGGHVQLADGSDLSYVEDGTACGPNMLCLDHRCLPASAFNFSTCPGSGERRICSHHGVCSNEGKCIC
  QPDWTGKDCSIHNPLPTSPPTGETERYKGPSGTNIIIGSIAGAVLVAAIVLGGTGWGFKNIRRGRSGGA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002390
• ORF Size: 2307 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002390.2, NM_002390.3, NM_002390.4, NM_002390.5, NP_002381.2
• RefSeq Size: 4402
• RefSeq ORF: 2310
• Locus ID: 4185
• Protein Families: Druggable Genome, Transmembrane
• MW: 83.2 kDa
• Gene Summary: This gene encodes a member of the ADAM (a disintegrin and metalloprotease) protein family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. The encoded preproprotein is proteolytically processed to generate the mature protease. This gene represents a candidate tumor suppressor gene for human breast cancer based on its location within a minimal region of chromosome 17q21 previously defined by tumor deletion mapping. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed. [provided by RefSeq, Jan 2016]