DGAT2L4 (AWAT2) (NM_001002254) Human Tagged ORF Clone

CAT#: RC220971

AWAT2 (Myc-DDK-tagged)-Human acyl-CoA wax alcohol acyltransferase 2 (AWAT2)

Product Images

Western blot validation of overexpression lysate (Cat# LY424189) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC220971 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: AWAT2
• Synonyms: ARAT; DC4; DGAT2L4; MFAT; WS
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC220971 representing NM_001002254
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTCTTGCCCTCTAAGAAGGACCTCAAGACTGCCCTGGATGTCTTTGCTGTTTTCCAGTGGTCCTTCA
  GTGCCTTGCTTATCACAACCACTGTGATTGCTGTCAACCTCTACCTGGTGGTGTTCACACCATACTGGCC
  TGTCACTGTGCTTATTCTTACCTGGCTGGCTTTTGACTGGAAGACCCCTCAGCGAGGCGGCCGCCGGTTT
  ACCTGTGTGAGGCACTGGCGCCTGTGGAAACACTACAGCGATTATTTCCCTCTCAAGCTTCTGAAGACTC
  ATGACATCTGCCCCAGCCGCAACTACATCCTCGTCTGCCACCCTCATGGGCTCTTTGCCCATGGATGGTT
  TGGCCACTTTGCCACAGAGGCCTCAGGCTTCTCCAAGATATTTCCTGGCATCACCCCTTACATACTCACA
  CTGGGAGCCTTTTTCTGGATGCCTTTCCTCAGAGAATATGTAATGTCTACAGGGGCCTGCTCTGTGAGTC
  GATCCTCCATTGACTTTCTGCTGACTCATAAAGGCACAGGCAACATGGTCATTGTGGTGATTGGTGGACT
  GGCTGAGTGCAGATACAGCCTGCCAGGTTCTTCTACCCTGGTGTTGAAGAACCGGTCTGGCTTTGTGCGC
  ATGGCCCTTCAGCATGGGGTGCCTCTAATACCTGCCTATGCCTTTGGGGAGACGGACCTCTATGATCAGC
  ACATTTTCACTCCTGGTGGCTTTGTCAACCGCTTCCAGAAGTGGTTCCAGAGCATGGTTCACATCTACCC
  TTGTGCTTTCTATGGACGTGGCTTCACCAAGAACTCCTGGGGCCTTCTGCCCTATAGTCGGCCTGTAACC
  ACCATCGTCGGGGAGCCTCTACCAATGCCCAAGATTGAGAATCCAAGCCAGGAGATCGTGGCTAAATATC
  ACACACTCTATATTGATGCCCTACGTAAACTGTTTGACCAGCATAAGACCAAGTTTGGTATCTCAGAGAC
  CCAGGAGCTGGAGATAATT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC220971 representing NM_001002254
  Red=Cloning site Green=Tags(s)
  
  MLLPSKKDLKTALDVFAVFQWSFSALLITTTVIAVNLYLVVFTPYWPVTVLILTWLAFDWKTPQRGGRRF
  TCVRHWRLWKHYSDYFPLKLLKTHDICPSRNYILVCHPHGLFAHGWFGHFATEASGFSKIFPGITPYILT
  LGAFFWMPFLREYVMSTGACSVSRSSIDFLLTHKGTGNMVIVVIGGLAECRYSLPGSSTLVLKNRSGFVR
  MALQHGVPLIPAYAFGETDLYDQHIFTPGGFVNRFQKWFQSMVHIYPCAFYGRGFTKNSWGLLPYSRPVT
  TIVGEPLPMPKIENPSQEIVAKYHTLYIDALRKLFDQHKTKFGISETQELEII
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001002254
• ORF Size: 999 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001002254.1, NP_001002254.1
• RefSeq Size: 2124 bp
• RefSeq ORF: 1002 bp
• Locus ID: 158835
• Protein Families: Transmembrane
• Protein Pathways: Glycerolipid metabolism, Metabolic pathways, Retinol metabolism
• MW: 37.9 kDa
• Gene Summary: This gene encodes an enzyme belonging to the diacylglycerol acyltransferase family. This enzyme produces wax esters by the esterification of long chain (or wax) alcohols with acyl-CoA-derived fatty acids. It functions in lipid metabolism in the skin, mostly in undifferentiated peripheral sebocytes. This enzyme may also have acyl-CoA:retinol acyltransferase activities, where it catalyzes the synthesis of diacylglycerols and retinyl esters. [provided by RefSeq, May 2010]