DGAT2L4 (AWAT2) (NM_001002254) Human Tagged ORF Clone

CAT#: RG220971

AWAT2 (GFP-tagged) - Human acyl-CoA wax alcohol acyltransferase 2 (AWAT2)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: AWAT2
• Synonyms: ARAT; DC4; DGAT2L4; MFAT; WS
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG220971 representing NM_001002254
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTCTTGCCCTCTAAGAAGGACCTCAAGACTGCCCTGGATGTCTTTGCTGTTTTCCAGTGGTCCTTCA
  GTGCCTTGCTTATCACAACCACTGTGATTGCTGTCAACCTCTACCTGGTGGTGTTCACACCATACTGGCC
  TGTCACTGTGCTTATTCTTACCTGGCTGGCTTTTGACTGGAAGACCCCTCAGCGAGGCGGCCGCCGGTTT
  ACCTGTGTGAGGCACTGGCGCCTGTGGAAACACTACAGCGATTATTTCCCTCTCAAGCTTCTGAAGACTC
  ATGACATCTGCCCCAGCCGCAACTACATCCTCGTCTGCCACCCTCATGGGCTCTTTGCCCATGGATGGTT
  TGGCCACTTTGCCACAGAGGCCTCAGGCTTCTCCAAGATATTTCCTGGCATCACCCCTTACATACTCACA
  CTGGGAGCCTTTTTCTGGATGCCTTTCCTCAGAGAATATGTAATGTCTACAGGGGCCTGCTCTGTGAGTC
  GATCCTCCATTGACTTTCTGCTGACTCATAAAGGCACAGGCAACATGGTCATTGTGGTGATTGGTGGACT
  GGCTGAGTGCAGATACAGCCTGCCAGGTTCTTCTACCCTGGTGTTGAAGAACCGGTCTGGCTTTGTGCGC
  ATGGCCCTTCAGCATGGGGTGCCTCTAATACCTGCCTATGCCTTTGGGGAGACGGACCTCTATGATCAGC
  ACATTTTCACTCCTGGTGGCTTTGTCAACCGCTTCCAGAAGTGGTTCCAGAGCATGGTACACATCTACCC
  TTGTGCTTTCTATGGACGTGGCTTCACCAAGAACTCCTGGGGCCTTCTGCCCTATAGTCGGCCTGTAACC
  ACCATCGTCGGGGAGCCTCTACCAATGCCCAAGATTGAGAATCCAAGCCAGGAGATCGTGGCTAAATATC
  ACACACTCTATATTGATGCCCTACGTAAACTGTTTGACCAGCATAAGACCAAGTTTGGTATCTCAGAGAC
  CCAGGAGCTGGAGATAATT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG220971 representing NM_001002254
  Red=Cloning site Green=Tags(s)
  
  MLLPSKKDLKTALDVFAVFQWSFSALLITTTVIAVNLYLVVFTPYWPVTVLILTWLAFDWKTPQRGGRRF
  TCVRHWRLWKHYSDYFPLKLLKTHDICPSRNYILVCHPHGLFAHGWFGHFATEASGFSKIFPGITPYILT
  LGAFFWMPFLREYVMSTGACSVSRSSIDFLLTHKGTGNMVIVVIGGLAECRYSLPGSSTLVLKNRSGFVR
  MALQHGVPLIPAYAFGETDLYDQHIFTPGGFVNRFQKWFQSMVHIYPCAFYGRGFTKNSWGLLPYSRPVT
  TIVGEPLPMPKIENPSQEIVAKYHTLYIDALRKLFDQHKTKFGISETQELEII
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001002254
• ORF Size: 999 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001002254.1, NP_001002254.1
• RefSeq Size: 2124
• RefSeq ORF: 1002
• Locus ID: 158835
• Protein Families: Transmembrane
• Protein Pathways: Glycerolipid metabolism, Metabolic pathways, Retinol metabolism
• Gene Summary: This gene encodes an enzyme belonging to the diacylglycerol acyltransferase family. This enzyme produces wax esters by the esterification of long chain (or wax) alcohols with acyl-CoA-derived fatty acids. It functions in lipid metabolism in the skin, mostly in undifferentiated peripheral sebocytes. This enzyme may also have acyl-CoA:retinol acyltransferase activities, where it catalyzes the synthesis of diacylglycerols and retinyl esters. [provided by RefSeq, May 2010]