DPP10 (NM_001004360) Human Tagged ORF Clone

CAT#: RC221061

DPP10 (Myc-DDK-tagged)-Human dipeptidyl-peptidase 10 (non-functional) (DPP10), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: DPP10
• Synonyms: DPL2; DPPY; DPRP-3; DPRP3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC221061 representing NM_001004360
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGCAAGGTGGAGAGCCGCGGGGAAGGGGGCAGAGAGGAACTGGGAAGTAACAGCCCTCCACAGAGAA
  ACTGGAAGGGAATTGCTATTGCTCTGCTGGTGATTTTAGTTGTATGCTCACTCATCACTATGTCAGTCAT
  CCTCTTAACCCCAGATGAACTCACAAATTCGTCAGAAACCAGATTGTCTTTGGAAGACCTCTTTAGGAAA
  GACTTTGTGCTTCACGATCCAGAGGCTCGGTGGATCAATGATACAGATGTGGTGTATAAAAGCGAGAATG
  GACATGTCATTAAACTGAATATAGAAACAAATGCTACCACATTATTATTGGAAAACACAACTTTTGTAAC
  CTTCAAAGCATCAAGACATTCAGTTTCACCAGATTTAAAATATGTCCTTCTGGCATATGATGTCAAACAG
  ATTTTTCATTATTCGTATACTGCTTCATATGTGATTTACAACATACACACTAGGGAAGTTTGGGAGTTAA
  ATCCTCCAGAAGTAGAGGACTCCGTCTTGCAGTACGCGGCCTGGGGTGTCCAAGGGCAGCAGCTGATTTA
  TATTTTTGAAAATAATATCTACTATCAACCTGATATAAAGAGCAGTTCATTGCGACTGACATCTTCTGGA
  AAAGAAGAAATAATTTTTAATGGGATTGCTGACTGGTTATATGAAGAGGAACTCCTGCATTCTCACATCG
  CCCACTGGTGGTCACCAGATGGAGAAAGACTTGCCTTCCTGATGATAAATGACTCTTTGGTACCCACCAT
  GGTTATCCCTCGGTTTACTGGAGCGTTGTATCCCAAAGGAAAGCAGTATCCGTATCCTAAGGCAGGTCAA
  GTGAACCCAACAATAAAATTATATGTTGTAAACCTGTATGGACCAACTCACACTTTGGAGCTCATGCCAC
  CTGACAGCTTTAAATCAAGAGAATACTATATCACTATGGTTAAATGGGTAAGCAATACCAAGACTGTGGT
  AAGATGGTTAAACCGAGCTCAGAACATCTCCATCCTCACAGTCTGTGAGACCACTACAGGTGCTTGTAGT
  AAAAAATATGAGATGACATCAGATACGTGGCTCTCTCAGCAGAATGAGGAGCCCGTGTTTTCTAGAGACG
  GCAGCAAATTCTTTATGACAGTGCCTGTTAAGCAAGGGGGACGTGGAGAATTTCACCACATAGCTATGTT
  CCTCATCCAGAGTAAAAGTGAGCAAATTACCGTGCGGCATCTGACATCAGGAAACTGGGAAGTGATAAAG
  ATCTTGGCATACGATGAAACTACTCAAAAAATTTACTTTCTGAGCACTGAATCTTCTCCCAGAGGAAGGC
  AGCTGTACAGTGCTTCTACTGAAGGATTATTGAATCGCCAATGCATTTCATGTAATTTCATGAAAGAACA
  ATGTACATATTTTGATGCCAGTTTTAGTCCCATGAATCAACATTTCTTATTATTCTGTGAAGGTCCAAGG
  GTCCCAGTGGTCAGCCTACATAGTACGGACAACCCAGCAAAATATTTTATATTGGAAAGCAATTCTATGC
  TGAAGGAAGCTATCCTGAAGAAGAAGATAGGAAAGCCAGAAATTAAAATCCTTCATATTGACGACTATGA
  ACTTCCTTTACAGTTGTCCCTTCCCAAAGATTTTATGGACCGAAACCAGTATGCTCTTCTGTTAATAATG
  GATGAAGAACCAGGAGGCCAGCTGGTTACAGATAAGTTCCATATTGACTGGGATTCCGTACTCATTGACA
  TGGATAATGTCATTGTAGCAAGATTTGATGGCAGAGGAAGTGGATTCCAGGGTCTGAAAATTTTGCAGGA
  GATTCATCGAAGATTAGGTTCAGTAGAAGTAAAGGACCAAATAACAGCTGTGAAATTTTTGCTGAAACTG
  CCTTACATTGACTCCAAAAGATTAAGCATTTTTGGAAAGGGTTATGGTGGCTATATTGCATCAATGATCT
  TAAAATCAGATGAAAAGCTTTTTAAATGTGGATCCGTGGTTGCACCTATCACAGACTTGAAATTGTATGC
  CTCAGCTTTCTCTGAAAGATACCTTGGGATGCCATCTAAGGAAGAAAGCACTTACCAGGCAGCCAGTGTG
  CTACATAATGTTCATGGCTTGAAAGAAGAAAATATATTAATAATTCATGGAACTGCTGACACAAAAGTTC
  ATTTCCAACACTCAGCAGAATTAATCAAGCACCTAATAAAAGCTGGAGTGAATTATACTATGCAGGTCTA
  CCCAGATGAAGGTCATAACGTATCTGAGAAGAGCAAGTATCATCTCTACAGCACAATCCTCAAATTCTTC
  AGTGATTGTTTGAAGGAAGAAATATCTGTGCTACCACAGGAACCAGAAGAAGATGAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC221061 representing NM_001004360
  Red=Cloning site Green=Tags(s)
  
  MRKVESRGEGGREELGSNSPPQRNWKGIAIALLVILVVCSLITMSVILLTPDELTNSSETRLSLEDLFRK
  DFVLHDPEARWINDTDVVYKSENGHVIKLNIETNATTLLLENTTFVTFKASRHSVSPDLKYVLLAYDVKQ
  IFHYSYTASYVIYNIHTREVWELNPPEVEDSVLQYAAWGVQGQQLIYIFENNIYYQPDIKSSSLRLTSSG
  KEEIIFNGIADWLYEEELLHSHIAHWWSPDGERLAFLMINDSLVPTMVIPRFTGALYPKGKQYPYPKAGQ
  VNPTIKLYVVNLYGPTHTLELMPPDSFKSREYYITMVKWVSNTKTVVRWLNRAQNISILTVCETTTGACS
  KKYEMTSDTWLSQQNEEPVFSRDGSKFFMTVPVKQGGRGEFHHIAMFLIQSKSEQITVRHLTSGNWEVIK
  ILAYDETTQKIYFLSTESSPRGRQLYSASTEGLLNRQCISCNFMKEQCTYFDASFSPMNQHFLLFCEGPR
  VPVVSLHSTDNPAKYFILESNSMLKEAILKKKIGKPEIKILHIDDYELPLQLSLPKDFMDRNQYALLLIM
  DEEPGGQLVTDKFHIDWDSVLIDMDNVIVARFDGRGSGFQGLKILQEIHRRLGSVEVKDQITAVKFLLKL
  PYIDSKRLSIFGKGYGGYIASMILKSDEKLFKCGSVVAPITDLKLYASAFSERYLGMPSKEESTYQAASV
  LHNVHGLKEENILIIHGTADTKVHFQHSAELIKHLIKAGVNYTMQVYPDEGHNVSEKSKYHLYSTILKFF
  SDCLKEEISVLPQEPEEDE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001004360
• ORF Size: 2367 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001004360.1, NM_001004360.2, NM_001004360.3, NP_001004360.1, NP_001004360.2
• RefSeq Size: 4902 bp
• RefSeq ORF: 2370 bp
• Locus ID: 57628
• Cytogenetics: 2q14.1
• Protein Families: Druggable Genome, Protease, Transmembrane
• MW: 90.2 kDa
• Gene Summary: This gene encodes a single-pass type II membrane protein that is a member of the S9B family in clan SC of the serine proteases. This protein has no detectable protease activity, most likely due to the absence of the conserved serine residue normally present in the catalytic domain of serine proteases. However, it does bind specific voltage-gated potassium channels and alters their expression and biophysical properties. Mutations in this gene have been associated with asthma. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]