DCP2 (NM_152624) Human Tagged ORF Clone

CAT#: RC221165

DCP2 (Myc-DDK-tagged)-Human DCP2 decapping enzyme homolog (S. cerevisiae) (DCP2)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: DCP2
• Synonyms: NUDT20
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC221165 representing NM_152624
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGACCAAACGGGTGGAGATTCCCGGCAGCGTCCTGGACGATCTCTGCAGCCGATTTATTTTGCATA
  TTCCCAGCGAGGAAAGAGACAATGCAATCCGAGTGTGTTTTCAGATTGAACTTGCCCATTGGTTTTACTT
  GGATTTCTACATGCAGAACACACCAGGATTACCTCAGTGTGGGATAAGAGACTTTGCTAAAGCTGTCTTC
  AGTCATTGTCCGTTTTTGCTGCCTCAAGGTGAAGATGTGGAAAAAGTTTTGGATGAATGGAAGGAATATA
  AAATGGGAGTACCAACATATGGTGCAATTATTCTTGATGAGACACTTGAAAATGTACTACTAGTTCAGGG
  GTACCTAGCAAAATCAGGCTGGGGATTTCCAAAAGGAAAAGTAAATAAAGAAGAAGCTCCTCATGATTGT
  GCTGCTAGAGAGGTCTTTGAAGAAACTGGTTTTGATATCAAAGACTATATTTGTAAGGATGATTACATTG
  AACTTCGAATCAATGACCAGCTTGCTCGTTTGTACATCATTCCAGGAATTCCAAAAGACACAAAATTTAA
  CCCAAAAACTAGAAGAGAAATTCGGAACATTGAGTGGTTCTCTATTGAGAAATTGCCTTGTCATAGAAAT
  GATATGACCCCCAAATCCAAACTTGGTTTGGCACCTAACAAATTTTTTATGGCCATTCCCTTTATCAGAC
  CATTAAGGGACTGGCTTTCTCGAAGATTTGGCGATTCCTCAGACAGTGACAATGGATTTTCCTCAACTGG
  TAGCACGCCGGCTAAACCCACTGTGGAAAAATTGAGTCGAACCAAATTCCGCCACAGTCAGCAGTTATTT
  CCTGACGGTTCTCCTGGTGACCAGTGGGTAAAGCACAGGCAACCACTGCAGCAAAAGCCATATAATAATC
  ATTCTGAAATGTCTGACCTTTTAAAAGGAAAGAATCAAAGTATGAGGGGAAATGGCAGAAAACAGTATCA
  AGATTCACCTAATCAAAAGAAAAGAACAAATGGGCTTCAGCCAGCAAAGCAGCAGAATTCTTTGATGAAG
  TGTGAAAAGAAACTTCATCCACGGAAACTTCAGGATAATTTTGAAACAGATGCTGTATATGACTTGCCTA
  GCTCCAGTGAAGACCAGTTGCTAGAACATGCTGAGGGACAGCCCGTGGCATGTAATGGACATTGCAAGTT
  CCCCTTTTCATCCAGAGCCTTTTTGAGTTTCAAGTTTGACCATAATGCTATAATGAAAATCTTGGACCTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC221165 representing NM_152624
  Red=Cloning site Green=Tags(s)
  
  METKRVEIPGSVLDDLCSRFILHIPSEERDNAIRVCFQIELAHWFYLDFYMQNTPGLPQCGIRDFAKAVF
  SHCPFLLPQGEDVEKVLDEWKEYKMGVPTYGAIILDETLENVLLVQGYLAKSGWGFPKGKVNKEEAPHDC
  AAREVFEETGFDIKDYICKDDYIELRINDQLARLYIIPGIPKDTKFNPKTRREIRNIEWFSIEKLPCHRN
  DMTPKSKLGLAPNKFFMAIPFIRPLRDWLSRRFGDSSDSDNGFSSTGSTPAKPTVEKLSRTKFRHSQQLF
  PDGSPGDQWVKHRQPLQQKPYNNHSEMSDLLKGKNQSMRGNGRKQYQDSPNQKKRTNGLQPAKQQNSLMK
  CEKKLHPRKLQDNFETDAVYDLPSSSEDQLLEHAEGQPVACNGHCKFPFSSRAFLSFKFDHNAIMKILDL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_152624
• ORF Size: 1260 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_152624.1, NM_152624.2, NM_152624.3, NM_152624.4, NM_152624.5, NP_689837.2
• RefSeq Size: 10198
• RefSeq ORF: 1263
• Locus ID: 167227
• Domains: NUDIX, DCP2
• Protein Pathways: RNA degradation
• MW: 48.9 kDa
• Gene Summary: The protein encoded by this gene is a key component of an mRNA-decapping complex required for degradation of mRNAs, both in normal mRNA turnover, and in nonsense-mediated mRNA decay (NMD). It removes the 7-methyl guanine cap structure from mRNA, prior to its degradation from the 5' end. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Jun 2011]