CRLF2 (NM_022148) Human Tagged ORF Clone
CAT#: RC221190
CRLF2 (Myc-DDK-tagged)-Human cytokine receptor-like factor 2 (CRLF2), transcript variant 1
"NM_022148" in other vectors (6)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | CRLF2 |
Synonyms | CRL2; CRLF2Y; TSLPR |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC221190 representing NM_022148
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGCGGCTGGTTCTGCTGTGGGGAGCTGCCGTCTTTCTGCTGGGAGGCTGGATGGCTTTGGGGCAAG GAGGAGCAGAAGGAGTACAGATTCAGATCATCTACTTCAATTTAGAAACCGTGCAGGTGACATGGAATGC CAGCAAATACTCCAGGACCAACCTGACTTTCCACTACAGATTCAACGGTGATGAGGCCTATGACCAGTGC ACCAACTACCTTCTCCAGGAAGGTCACACTTCGGGGTGCCTCCTAGACGCAGAGCAGCGAGACGACATTC TCTATTTCTCCATCAGGAATGGGACGCACCCCGTTTTCACCGCAAGTCGCTGGATGGTTTATTACCTGAA ACCCAGTTCCCCGAAGCACGTGAGATTTTCGTGGCATCAGGATGCAGTGACGGTGACGTGTTCTGACCTG TCCTACGGGGATCTCCTCTATGAGGTTCAGTACCGGAGCCCCTTCGACACCGAGTGGCAGTCCAAACAGG AAAATACCTGCAACGTCACCATAGAAGGCTTGGATGCCGAGAAGTGTTACTCTTTCTGGGTCAGGGTGAA GGCTATGGAGGATGTATATGGGCCAGACACATACCCAAGCGACTGGTCAGAGGTGACATGCTGGCAGAGA GGCGAGATTCGGGATGCCTGTGCAGAGACACCAACGCCTCCCAAACCAAAGCTGTCCAAATTTATTTTAA TTTCCAGCCTGGCCATCCTTCTGATGGTGTCTCTCCTCCTTCTGTCTTTATGGAAATTATGGAGAGTGAG GAAGTTTCTCATTCCCAGCGTGCCAGACCCGAAATCCATCTTCCCCGGGCTCTTTGAGATACACCAAGGG AACTTCCAGGAGTGGATCACAGACACCCAGAACGTGGCCCACCTCCACAAGATGGCAGGTGCAGAGCAAG AAAGTGGCCCCGAGGAGCCCCTGGTAGTCCAGTTGGCCAAGACTGAAGCCGAGTCTCCCAGGATGCTGGA CCCACAGACCGAGGAGAAAGAGGCCTCTGGGGGATCCCTCCAGCTTCCCCACCAGCCCCTCCAAGGCGGT GATGTGGTCACAATCGGGGGCTTCACCTTTGTGATGAATGACCGCTCCTACGTGGCGTTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC221190 representing NM_022148
Red=Cloning site Green=Tags(s) MGRLVLLWGAAVFLLGGWMALGQGGAEGVQIQIIYFNLETVQVTWNASKYSRTNLTFHYRFNGDEAYDQC TNYLLQEGHTSGCLLDAEQRDDILYFSIRNGTHPVFTASRWMVYYLKPSSPKHVRFSWHQDAVTVTCSDL SYGDLLYEVQYRSPFDTEWQSKQENTCNVTIEGLDAEKCYSFWVRVKAMEDVYGPDTYPSDWSEVTCWQR GEIRDACAETPTPPKPKLSKFILISSLAILLMVSLLLLSLWKLWRVRKFLIPSVPDPKSIFPGLFEIHQG NFQEWITDTQNVAHLHKMAGAEQESGPEEPLVVQLAKTEAESPRMLDPQTEEKEASGGSLQLPHQPLQGG DVVTIGGFTFVMNDRSYVAL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_022148 |
ORF Size | 2798 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_022148.2, NM_022148.3, NP_071431.2 |
RefSeq Size | 1579 bp |
RefSeq ORF | 1116 bp |
Locus ID | 64109 |
Protein Families | Druggable Genome, Secreted Protein, Transmembrane |
Protein Pathways | Cytokine-cytokine receptor interaction, Jak-STAT signaling pathway |
MW | 41.8 kDa |
Gene Summary | This gene encodes a member of the type I cytokine receptor family. The encoded protein is a receptor for thymic stromal lymphopoietin (TSLP). Together with the interleukin 7 receptor (IL7R), the encoded protein and TSLP activate STAT3, STAT5, and JAK2 pathways, which control processes such as cell proliferation and development of the hematopoietic system. Rearrangement of this gene with immunoglobulin heavy chain gene (IGH) on chromosome 14, or with P2Y purinoceptor 8 gene (P2RY8) on the same X or Y chromosomes is associated with B-progenitor acute lymphoblastic leukemia (ALL) and Down syndrome ALL. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Sep 2014] |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC305006 | CRLF2 (untagged)-Human cytokine receptor-like factor 2 (CRLF2), transcript variant 1 |
USD 640.00 |
|
RG221190 | CRLF2 (GFP-tagged) - Human cytokine receptor-like factor 2 (CRLF2), transcript variant 1 |
USD 460.00 |
|
RC221190L1 | Lenti ORF clone of Human cytokine receptor-like factor 2 (CRLF2), transcript variant 1, Myc-DDK-tagged |
USD 768.00 |
|
RC221190L2 | Lenti ORF clone of Human cytokine receptor-like factor 2 (CRLF2), transcript variant 1, mGFP tagged |
USD 620.00 |
|
RC221190L3 | Lenti ORF clone of Human cytokine receptor-like factor 2 (CRLF2), transcript variant 1, Myc-DDK-tagged |
USD 620.00 |
|
RC221190L4 | Lenti ORF clone of Human cytokine receptor-like factor 2 (CRLF2), transcript variant 1, mGFP tagged |
USD 620.00 |
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