LIPF (NM_004190) Human Tagged ORF Clone

CAT#: RC221225

LIPF (Myc-DDK-tagged)-Human lipase, gastric (LIPF), transcript variant 2

Product Images

Western blot validation of overexpression lysate (Cat# LY401348) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC221225 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified LIPF protein (Cat# TP321225). The protein was produced from HEK293T cells transfected with LIPF cDNA clone (Cat# RC221225) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: LIPF
• Synonyms: GL; HGL; HLAL
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC221225 representing NM_004190
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGGCTGCTTTTAACAATGGCAAGTTTGATATCTGTACTGGGGACTACACATGGTTTGTTTGGAAAAT
  TACATCCTGGAAGCCCTGAAGTGACTATGAACATTAGTCAGATGATTACTTATTGGGGATACCCAAATGA
  AGAATATGAAGTTGTGACTGAAGATGGTTATATTCTTGAAGTCAATAGAATTCCTTATGGGAAGAAAAAT
  TCAGGGAATACAGGCCAGAGACCTGTTGTGTTTTTGCAGCATGGTTTGCTTGCATCAGCCACAAACTGGA
  TTTCCAACCTGCCGAACAACAGCCTTGCCTTCATTCTGGCAGATGCTGGTTATGATGTGTGGCTGGGCAA
  CAGCAGAGGAAACACCTGGGCCAGAAGAAACTTGTACTATTCACCAGATTCAGTTGAATTCTGGGCTTTC
  AGCTTTGATGAAATGGCTAAATATGACCTTCCAGCCACAATCGACTTCATTGTAAAGAAAACTGGACAGA
  AGCAGCTACACTATGTTGGCCATTCCCAGGGCACCACCATTGGTTTTATTGCCTTTTCCACCAATCCCAG
  CCTGGCTAAAAGAATCAAAACCTTCTATGCTCTAGCTCCTGTTGCCACTGTGAAGTATACAAAAAGCCTT
  ATAAACAAACTTAGATTTGTTCCTCAATCCCTCTTCAAGTTTATATTTGGTGACAAAATATTCTACCCAC
  ACAACTTCTTTGATCAATTTCTTGCTACTGAAGTGTGCTCCCGTGAGATGCTGAATCTCCTTTGCAGCAA
  TGCCTTATTTATAATTTGTGGATTTGACAGTAAGAACTTTAACACGAGTCGCTTGGATGTGTATCTATCA
  CATAATCCAGCAGGAACTTCTGTTCAAAACATGTTCCATTGGACCCAGGCTGTTAAGTCTGGGAAATTCC
  AAGCTTATGACTGGGGAAGCCCAGTTCAGAATAGGATGCACTATGATCAGTCCCAACCTCCCTACTACAA
  TGTGACAGCCATGAATGTACCAATTGCAGTGTGGAACGGTGGCAAGGACCTGTTGGCTGACCCCCAAGAT
  GTTGGCCTTTTGCTTCCAAAACTCCCCAATCTTATTTACCACAAGGAGATTCCTTTTTACAATCACTTGG
  ACTTTATCTGGGCAATGGATGCCCCTCAAGAAGTTTACAATGACATTGTTTCTATGATATCAGAAGATAA
  AAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC221225 representing NM_004190
  Red=Cloning site Green=Tags(s)
  
  MWLLLTMASLISVLGTTHGLFGKLHPGSPEVTMNISQMITYWGYPNEEYEVVTEDGYILEVNRIPYGKKN
  SGNTGQRPVVFLQHGLLASATNWISNLPNNSLAFILADAGYDVWLGNSRGNTWARRNLYYSPDSVEFWAF
  SFDEMAKYDLPATIDFIVKKTGQKQLHYVGHSQGTTIGFIAFSTNPSLAKRIKTFYALAPVATVKYTKSL
  INKLRFVPQSLFKFIFGDKIFYPHNFFDQFLATEVCSREMLNLLCSNALFIICGFDSKNFNTSRLDVYLS
  HNPAGTSVQNMFHWTQAVKSGKFQAYDWGSPVQNRMHYDQSQPPYYNVTAMNVPIAVWNGGKDLLADPQD
  VGLLLPKLPNLIYHKEIPFYNHLDFIWAMDAPQEVYNDIVSMISEDKK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004190
• ORF Size: 1194 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004190.1, NM_004190.2, NM_004190.3, NP_004181.1
• RefSeq Size: 1365 bp
• RefSeq ORF: 1197 bp
• Locus ID: 8513
• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: Glycerolipid metabolism, Metabolic pathways
• MW: 45.24 kDa
• Gene Summary: This gene encodes gastric lipase, an enzyme involved in the digestion of dietary triglycerides in the gastrointestinal tract, and responsible for 30% of fat digestion processes occurring in human. It is secreted by gastric chief cells in the fundic mucosa of the stomach, and it hydrolyzes the ester bonds of triglycerides under acidic pH conditions. The gene is a member of a conserved gene family of lipases that play distinct roles in neutral lipid metabolism. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2010]