A4GNT (NM_016161) Human Tagged ORF Clone

CAT#: RC221256

A4GNT (Myc-DDK-tagged)-Human alpha-1,4-N-acetylglucosaminyltransferase (A4GNT)

Product Images

Western blot validation of overexpression lysate (Cat# LY414153) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC221256 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: A4GNT
• Synonyms: alpha4GnT
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC221256 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGGAAGGAGCTCCAGCTCTCCCTGTCAGTCACCTTGCTGCTTGTCTGTGGCTTCCTCTACCAGTTCA
  CCCTGAAGTCCAGCTGCCTCTTCTGCTTGCCTTCTTTCAAGTCCCACCAGGGGCTGGAAGCCCTCCTGAG
  CCACAGACGTGGCATTGTGTTTCTAGAGACCTCAGAGAGAATGGAGCCACCCCATTTGGTCTCCTGTTCC
  GTAGAGTCTGCTGCCAAGATTTATCCTGAGTGGCCTGTGGTGTTCTTTATGAAGGGTCTTACTGATTCCA
  CACCGATGCCCTCAAACTCCACATACCCAGCTTTTTCCTTCCTGTCAGCAATAGACAACGTTTTCCTCTT
  CCCTTTGGATATGAAAAGGCTGCTTGAAGACACACCATTGTTTTCATGGTACAATCAAATCAACGCCAGC
  GCAGAGAGAAACTGGCTCCACATCAGCTCGGATGCATCCCGCCTGGCCATCATCTGGAAATACGGTGGCA
  TCTACATGGACACCGATGTCATCTCCATCAGGCCCATCCCTGAGGAGAACTTTTTGGCTGCGCAGGCTTC
  TCGGTACTCTAGTAATGGAATATTTGGGTTCCTCCCCCACCACCCCTTTTTGTGGGAATGCATGGAAAAC
  TTTGTTGAACACTATAATTCAGACATTTGGGGCAACCAAGGCCCTGAGTTGATGACAAGGATGTTGAGGG
  TATGGTGTAAACTTGAAGACTTCCAGGAGGTGAGCGACCTCAGGTGTCTGAACATATCCTTCTTACACCC
  CCAAAGATTTTACCCCATCTCCTATCGAGAGTGGAGGCGCTACTATGAAGTGTGGGATACAGAGCCAAGC
  TTCAATGTCTCTTATGCCCTGCATTTGTGGAACCACATGAACCAGGAGGGGCGGGCTGTGATTAGAGGAA
  GCAACACACTGGTGGAAAATCTCTATCGCAAGCACTGTCCCAGGACTTACAGGGACCTGATTAAAGGCCC
  AGAGGGGTCAGTGACTGGGGAGCTGGGTCCAGGTAACAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC221256 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MRKELQLSLSVTLLLVCGFLYQFTLKSSCLFCLPSFKSHQGLEALLSHRRGIVFLETSERMEPPHLVSCS
  VESAAKIYPEWPVVFFMKGLTDSTPMPSNSTYPAFSFLSAIDNVFLFPLDMKRLLEDTPLFSWYNQINAS
  AERNWLHISSDASRLAIIWKYGGIYMDTDVISIRPIPEENFLAAQASRYSSNGIFGFLPHHPFLWECMEN
  FVEHYNSDIWGNQGPELMTRMLRVWCKLEDFQEVSDLRCLNISFLHPQRFYPISYREWRRYYEVWDTEPS
  FNVSYALHLWNHMNQEGRAVIRGSNTLVENLYRKHCPRTYRDLIKGPEGSVTGELGPGNK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_016161
• ORF Size: 1020 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016161.1, NM_016161.2, NP_057245.1
• RefSeq Size: 1771 bp
• RefSeq ORF: 1023 bp
• Locus ID: 51146
• Protein Families: Transmembrane
• MW: 39.5 kDa
• Gene Summary: This gene encodes a protein from the glycosyltransferase 32 family. The enzyme catalyzes the transfer of N-acetylglucosamine (GlcNAc) to core 2 branched O-glycans. It forms a unique glycan, GlcNAcalpha1-->4Galbeta-->R and is largely associated with the Golgi apparatus membrane. [provided by RefSeq, Jul 2008]