PTF1A (NM_178161) Human Tagged ORF Clone
CAT#: RC221496
PTF1A (Myc-DDK-tagged)-Human pancreas specific transcription factor, 1a (PTF1A)
"NM_178161" in other vectors (6)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | PTF1A |
Synonyms | bHLHa29; p48; PACA; PAGEN2; PTF1-p48 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC221496 representing NM_178161
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACGCGGTGTTGCTGGAGCACTTCCCCGGGGGCCTAGACGCCTTTCCTTCTTCGTACTTCGACGAGG ACGACTTCTTCACCGACCAGTCTTCACGGGACCCCCTGGAGGACGGCGATGAGCTGCTGGCGGACGAGCA GGCCGAGGTGGAGTTCCTTAGCCACCAGCTCCACGAGTACTGCTACCGCGACGGGGCGTGCCTGCTGCTG CAGCCCGCGCCCCCGGCCGCCCCGCTAGCGCTCGCCCCGCCGTCCTCGGGGGGCCTCGGTGAGCCAGACG ACGGCGGCGGCGGCGGCTACTGCTGCGAGACGGGGGCGCCCCCAGGCGGCTTCCCCTACTCGCCCGGCTC GCCGCCCTCGTGCCTGGCCTACCCGTGCGCCGGGGCGGCAGTACTGTCTCCCGGGGCGCGGCTGCGCGGC CTGAGCGGAGCGGCGGCTGCGGCGGCGCGGCGCCGGCGGCGGGTGCGCTCCGAGGCGGAGCTGCAGCAGC TGCGGCAGGCGGCCAACGTGCGCGAGCGGCGGCGCATGCAGTCCATCAACGACGCCTTCGAGGGGCTGCG CTCGCACATCCCCACGCTGCCCTACGAGAAGCGCCTCTCCAAGGTGGACACGCTGCGCCTGGCCATCGGC TACATCAACTTCCTCAGCGAGCTCGTGCAGGCCGACCTGCCCTTGCGCGGCGGTGGCGCGGGCGGCTGCG GGGGGCCGGGCGGCGGCGGGCGCCTGGGCGGGGACAGCCCGGGCAGCCAGGCCCAGAAGGTCATCATCTG CCATCGGGGCACCCGGTCCCCCTCCCCCAGCGACCCTGATTATGGCCTCCCTCCCCTAGCAGGACACTCT CTCTCATGGACTGATGAAAAACAACTCAAGGAACAAAATATTATCCGAACAGCCAAAGTCTGGACCCCAG AGGACCCCAGAAAACTCAACAGCAAATCTTCCTTCAACAACATAGAAAACGAACCACCATTTGAGTTTGT GTCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC221496 representing NM_178161
Red=Cloning site Green=Tags(s) MDAVLLEHFPGGLDAFPSSYFDEDDFFTDQSSRDPLEDGDELLADEQAEVEFLSHQLHEYCYRDGACLLL QPAPPAAPLALAPPSSGGLGEPDDGGGGGYCCETGAPPGGFPYSPGSPPSCLAYPCAGAAVLSPGARLRG LSGAAAAAARRRRRVRSEAELQQLRQAANVRERRRMQSINDAFEGLRSHIPTLPYEKRLSKVDTLRLAIG YINFLSELVQADLPLRGGGAGGCGGPGGGGRLGGDSPGSQAQKVIICHRGTRSPSPSDPDYGLPPLAGHS LSWTDEKQLKEQNIIRTAKVWTPEDPRKLNSKSSFNNIENEPPFEFVS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_178161 |
ORF Size | 984 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_178161.1, NM_178161.2, NP_835455.1 |
RefSeq Size | 987 bp |
RefSeq ORF | 987 bp |
Locus ID | 256297 |
Protein Families | Embryonic stem cells, ES Cell Differentiation/IPS |
MW | 34.8 kDa |
Gene Summary | This gene encodes a protein that is a component of the pancreas transcription factor 1 complex (PTF1) and is known to have a role in mammalian pancreatic development. The protein plays a role in determining whether cells allocated to the pancreatic buds continue towards pancreatic organogenesis or revert back to duodenal fates. The protein is thought to be involved in the maintenance of exocrine pancreas-specific gene expression including elastase 1 and amylase. Mutations in this gene cause cerebellar agenesis and loss of expression is seen in ductal type pancreas cancers. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC307134 | PTF1A (untagged)-Human pancreas specific transcription factor, 1a (PTF1A) |
USD 660.00 |
|
RG221496 | PTF1A (GFP-tagged) - Human pancreas specific transcription factor, 1a (PTF1A) |
USD 460.00 |
|
RC221496L1 | Lenti ORF clone of Human pancreas specific transcription factor, 1a (PTF1A), Myc-DDK-tagged |
USD 768.00 |
|
RC221496L2 | Lenti ORF clone of Human pancreas specific transcription factor, 1a (PTF1A), mGFP tagged |
USD 768.00 |
|
RC221496L3 | Lenti ORF clone of Human pancreas specific transcription factor, 1a (PTF1A), Myc-DDK-tagged |
USD 620.00 |
|
RC221496L4 | Lenti ORF clone of Human pancreas specific transcription factor, 1a (PTF1A), mGFP tagged |
USD 620.00 |
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