PTF1A (NM_178161) Human Tagged ORF Clone

CAT#: RG221496

PTF1A (GFP-tagged) - Human pancreas specific transcription factor, 1a (PTF1A)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: PTF1A
• Synonyms: bHLHa29; p48; PACA; PAGEN2; PTF1-p48
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG221496 representing NM_178161
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACGCGGTGTTGCTGGAGCACTTCCCCGGGGGCCTAGACGCCTTTCCTTCTTCGTACTTCGACGAGG
  ACGACTTCTTCACCGACCAGTCTTCACGGGACCCCCTGGAGGACGGCGATGAGCTGCTGGCGGACGAGCA
  GGCCGAGGTGGAGTTCCTTAGCCACCAGCTCCACGAGTACTGCTACCGCGACGGGGCGTGCCTGCTGCTG
  CAGCCCGCGCCCCCGGCCGCCCCGCTAGCGCTCGCCCCGCCGTCCTCGGGGGGCCTCGGTGAGCCAGACG
  ACGGCGGCGGCGGCGGCTACTGCTGCGAGACGGGGGCGCCCCCAGGCGGCTTCCCCTACTCGCCCGGCTC
  GCCGCCCTCGTGCCTGGCCTACCCGTGCGCCGGGGCGGCAGTACTGTCTCCCGGGGCGCGGCTGCGCGGC
  CTGAGCGGAGCGGCGGCTGCGGCGGCGCGGCGCCGGCGGCGGGTGCGCTCCGAGGCGGAGCTGCAGCAGC
  TGCGGCAGGCGGCCAACGTGCGCGAGCGGCGGCGCATGCAGTCCATCAACGACGCCTTCGAGGGGCTGCG
  CTCGCACATCCCCACGCTGCCCTACGAGAAGCGCCTCTCCAAGGTGGACACGCTGCGCCTGGCCATCGGC
  TACATCAACTTCCTCAGCGAGCTCGTGCAGGCCGACCTGCCCTTGCGCGGCGGTGGCGCGGGCGGCTGCG
  GGGGGCCGGGCGGCGGCGGGCGCCTGGGCGGGGACAGCCCGGGCAGCCAGGCCCAGAAGGTCATCATCTG
  CCATCGGGGCACCCGGTCCCCCTCCCCCAGCGACCCTGATTATGGCCTCCCTCCCCTAGCAGGACACTCT
  CTCTCATGGACTGATGAAAAACAACTCAAGGAACAAAATATTATCCGAACAGCCAAAGTCTGGACCCCAG
  AGGACCCCAGAAAACTCAACAGCAAATCTTCCTTCAACAACATAGAAAACGAACCACCATTTGAGTTTGT
  GTCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG221496 representing NM_178161
  Red=Cloning site Green=Tags(s)
  
  MDAVLLEHFPGGLDAFPSSYFDEDDFFTDQSSRDPLEDGDELLADEQAEVEFLSHQLHEYCYRDGACLLL
  QPAPPAAPLALAPPSSGGLGEPDDGGGGGYCCETGAPPGGFPYSPGSPPSCLAYPCAGAAVLSPGARLRG
  LSGAAAAAARRRRRVRSEAELQQLRQAANVRERRRMQSINDAFEGLRSHIPTLPYEKRLSKVDTLRLAIG
  YINFLSELVQADLPLRGGGAGGCGGPGGGGRLGGDSPGSQAQKVIICHRGTRSPSPSDPDYGLPPLAGHS
  LSWTDEKQLKEQNIIRTAKVWTPEDPRKLNSKSSFNNIENEPPFEFVS
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_178161
• ORF Size: 984 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_178161.1, NP_835455.1
• RefSeq Size: 987
• RefSeq ORF: 987
• Locus ID: 256297
• Protein Families: Embryonic stem cells, ES Cell Differentiation/IPS
• Gene Summary: This gene encodes a protein that is a component of the pancreas transcription factor 1 complex (PTF1) and is known to have a role in mammalian pancreatic development. The protein plays a role in determining whether cells allocated to the pancreatic buds continue towards pancreatic organogenesis or revert back to duodenal fates. The protein is thought to be involved in the maintenance of exocrine pancreas-specific gene expression including elastase 1 and amylase. Mutations in this gene cause cerebellar agenesis and loss of expression is seen in ductal type pancreas cancers. [provided by RefSeq, Jul 2008]