PANK1 (NM_148977) Human Tagged ORF Clone

CAT#: RC221538

PANK1 (Myc-DDK-tagged)-Human pantothenate kinase 1 (PANK1), transcript variant alpha

Product Images

Western blot validation of overexpression lysate (Cat# LY407715) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC221538 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified PANK1 protein (Cat# TP321538). The protein was produced from HEK293T cells transfected with PANK1 cDNA clone (Cat# RC221538) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PANK1
• Synonyms: PANK
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC221538 representing NM_148977
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTGAAACTCGTCGGTGGCGGTGGCGGGCAGGACTGGGCATGCTCAGTGGCGGGGACCAGTCTGGGAG
  GCGAGGAAGCCGCGTTTGAAGTCGCGCGGCCTGGGGATCAGGGGAAGGCGGGCGGCGGGAGCCCCGGCTG
  GGGGTGCGCGGGGATCCCCGATTCCGCGCCCGGGGCTGGCGTGCTCCAGGCCGGCGCCGTGGGGCCGGCG
  CGCGGGGGGCAGGGCGCAGAGGAGGTGGGGGAGTCGGCAGGAGGAGGGGAGGAGCGCCGGGTTCGCCATC
  CCCAGGCGCCGGCTCTGCGGCTGCTGAATCGGAAGCCGCAGGGAGGATCCGGGGAAATAAAGACGCCGGA
  GAATGACCTCCAGCGAGGCCGCCTGAGCCGGGGCCCGCGCACAGCCCCGCCAGCCCCCGGCATGGGCGAC
  CGCAGCGGGCAGCAGGAGCGCTCGGTCCCGCACTCTCCAGGGGCCCCCGTGGGCACCAGCGCCGCCGCTG
  TGAACGGGCTGCTGCACAACGGCTTCCATCCGCCGCCAGTCCAGCCGCCGCACGTCTGCAGCCGGGGTCC
  AGTGGGCGGCAGCGACGCGGCGCCCCAGCGCCTCCCGCTCCTGCCGGAGCTGCAGCCGCAGCCACTGCTC
  CCTCAGCATGACTCCCCGGCCAAGAAATGCCGGCTGCGGAGGAGGATGGACTCGGGGAGAAAGAACAGGC
  CGCCATTCCCATGGTTTGGCATGGACATCGGTGGAACGCTGGTTAAATTGGTGTATTTCGAGCCGAAGGA
  TATTACAGCCGAAGAGGAGCAAGAGGAAGTGGAGAACCTGAAGAGCATCCGGAAGTATTTGACTTCTAAT
  ACTGCTTATGGGAAAACTGGGATCCGAGACGTCCACCTGGAACTGAAAAACCTGACCATGTGTGGACGCA
  AAGGGAACCTGCACTTCATCCGCTTTCCCAGCTGTGCTATGCACAGGTTCATTCAGATGGGCAGCGAGAA
  GAACTTCTCTAGCCTTCACACCACCCTCTGTGCCACAGGAGGCGGGGCTTTCAAATTCGAAGAGGACTTC
  AGAATGATTGCTGACCTGCAGCTGCATAAACTGGATGAACTGGACTGTCTGATTCAGGGCCTGCTTTATG
  TCGACTCTGTTGGCTTCAACGGCAAGCCAGAATGTTACTATTTTGAAAATCCCACAAATCCTGAATTGTG
  TCAAAAAAAGCCGTACTGCCTTGATAACCCATACCCTATGTTGCTGGTTAACATGGGCTCAGGTGTCAGC
  ATTCTAGCCGTGTACTCCAAGGACAACTATAAAAGAGTTACAGGGACCAGTCTTGGAGGTGGAACATTCC
  TAGGCCTATGTTGCTTGCTGACTGGTTGTGAGACCTTTGAAGAAGCTCTGGAAATGGCAGCTAAAGGCGA
  CAGCACCAATGTTGATAAACTGGTGAAGGACATTTACGGAGGAGACTATGAACGATTTGGCCTTCAAGGA
  TCTGCTGTAGCATCAAGCTTTGGCAACATGATGAGTAAAGAAAAGCGAGATTCCATCAGCAAGGAAGACC
  TCGCCCGGGCCACATTGGTCACCATCACCAACAACATTGGCTCCATTGCTCGGATGTGCGCGTTGAATGA
  GAACATAGACAGAGTTGTGTTTGTTGGAAATTTTCTCAGAATCAATATGGTCTCCATGAAGCTGCTGGCA
  TATGCCATGGATTTTTGGTCCAAAGGACAACTGAAAGCTCTGTTTTTGGAACATGAGGGTTATTTTGGAG
  CCGTTGGGGCACTGTTGGAACTGTTCAAAATGACTGATGACAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC221538 representing NM_148977
  Red=Cloning site Green=Tags(s)
  
  MLKLVGGGGGQDWACSVAGTSLGGEEAAFEVARPGDQGKAGGGSPGWGCAGIPDSAPGAGVLQAGAVGPA
  RGGQGAEEVGESAGGGEERRVRHPQAPALRLLNRKPQGGSGEIKTPENDLQRGRLSRGPRTAPPAPGMGD
  RSGQQERSVPHSPGAPVGTSAAAVNGLLHNGFHPPPVQPPHVCSRGPVGGSDAAPQRLPLLPELQPQPLL
  PQHDSPAKKCRLRRRMDSGRKNRPPFPWFGMDIGGTLVKLVYFEPKDITAEEEQEEVENLKSIRKYLTSN
  TAYGKTGIRDVHLELKNLTMCGRKGNLHFIRFPSCAMHRFIQMGSEKNFSSLHTTLCATGGGAFKFEEDF
  RMIADLQLHKLDELDCLIQGLLYVDSVGFNGKPECYYFENPTNPELCQKKPYCLDNPYPMLLVNMGSGVS
  ILAVYSKDNYKRVTGTSLGGGTFLGLCCLLTGCETFEEALEMAAKGDSTNVDKLVKDIYGGDYERFGLQG
  SAVASSFGNMMSKEKRDSISKEDLARATLVTITNNIGSIARMCALNENIDRVVFVGNFLRINMVSMKLLA
  YAMDFWSKGQLKALFLEHEGYFGAVGALLELFKMTDDK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_148977
• ORF Size: 1794 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_148977.1, NM_148977.2, NP_683878.1
• RefSeq Size: 3367
• RefSeq ORF: 1797
• Locus ID: 53354
• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways, Pantothenate and CoA biosynthesis
• MW: 64.2 kDa
• Gene Summary: This gene encodes a member of the pantothenate kinase family. Pantothenate kinases are key regulatory enzymes in the biosynthesis of coenzyme A (CoA). The encoded protein catalyzes the first and rate-limiting enzymatic reaction in CoA biosynthesis and is regulated by CoA through feedback inhibition. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. This gene and an intronic miRNA on the same strand are co-regulated by the tumor suppressor p53 (see PMID 20833636). [provided by RefSeq, Apr 2011]