DAND5 (NM_152654) Human Tagged ORF Clone
CAT#: RC221703
DAND5 (Myc-DDK-tagged)-Human DAN domain family, member 5 (DAND5)
"NM_152654" in other vectors (6)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | DAND5 |
Synonyms | CER2; CERL2; CKTSF1B3; COCO; CRL2; DANTE; GREM3; SP1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC221703 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTCCTTGGCCAGCTATCCACTCTTCTGTGCCTGCTTAGCGGGGCCCTGCCTACAGGCTCAGGGAGGC CTGAACCCCAGTCTCCTCGACCTCAGTCCTGGGCTGCAGCCAATCAGACCTGGGCTCTGGGCCCAGGGGC CCTGCCCCCACTGGTGCCAGCTTCTGCCCTTGGGAGCTGGAAGGCCTTCTTGGGCCTGCAGAAAGCCAGG CAGCTGGGGATGGGCAGGCTGCAGCGTGGGCAAGACGAGGTGGCTGCTGTGACTCTGCCGCTGAACCCTC AGGAAGTGATCCAGGGGATGTGTAAGGCTGTGCCCTTCGTTCAGGTGTTCTCCCGGCCCGGCTGCTCAGC CATACGCCTCCGAAATCATCTGTGCTTTGGTCATTGCTCCTCTCTCTACATCCCTGGCTCGGACCCCACC CCACTAGTCCTGTGCAACAGCTGTATGCCTGCTCGCAAGCGTTGGGCACCCGTGGTCCTGTGGTGTCTCA CTGGCAGCTCAGCCTCCCGTCGACGGGTGAAGATATCCACCATGCTGATCGAGGGGTGTCACTGCAGCCC AAAAGCA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC221703 protein sequence
Red=Cloning site Green=Tags(s) MLLGQLSTLLCLLSGALPTGSGRPEPQSPRPQSWAAANQTWALGPGALPPLVPASALGSWKAFLGLQKAR QLGMGRLQRGQDEVAAVTLPLNPQEVIQGMCKAVPFVQVFSRPGCSAIRLRNHLCFGHCSSLYIPGSDPT PLVLCNSCMPARKRWAPVVLWCLTGSSASRRRVKISTMLIEGCHCSPKA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_152654 |
ORF Size | 567 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_152654.1, NM_152654.2, NP_689867.1 |
RefSeq Size | 1732 bp |
RefSeq ORF | 570 bp |
Locus ID | 199699 |
MW | 20.2 kDa |
Gene Summary | This gene encodes a member of the BMP (bone morphogenic protein) antagonist family. Like BMPs, BMP antagonists contain cystine knots and typically form homo- and heterodimers. The CAN (cerberus and dan) subfamily of BMP antagonists, to which this gene belongs, is characterized by a C-terminal cystine knot with an eight-membered ring. The antagonistic effect of the secreted protein encoded by this gene is likely due to its direct binding to BMP proteins. As an antagonist of BMP, this gene may play a role in regulating organogenesis, body patterning, and tissue differentiation. In mouse, this protein has been shown to bind Nodal and to inhibit the Nodal signaling pathway which patterns left/right body asymmetry. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC306475 | DAND5 (untagged)-Human DAN domain family, member 5 (DAND5) |
USD 420.00 |
|
RG221703 | DAND5 (GFP-tagged) - Human DAN domain family, member 5 (DAND5) |
USD 460.00 |
|
RC221703L1 | Lenti ORF clone of Human DAN domain family, member 5 (DAND5), Myc-DDK-tagged |
USD 620.00 |
|
RC221703L2 | Lenti ORF clone of Human DAN domain family, member 5 (DAND5), mGFP tagged |
USD 620.00 |
|
RC221703L3 | Lenti ORF clone of Human DAN domain family, member 5 (DAND5), Myc-DDK-tagged |
USD 620.00 |
|
RC221703L4 | Lenti ORF clone of Human DAN domain family, member 5 (DAND5), mGFP tagged |
USD 620.00 |
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