Pancreatic Lipase Related Protein 2 (PNLIPRP2) (NM_005396) Human Tagged ORF Clone

CAT#: RC221745

PNLIPRP2 (Myc-DDK-tagged)-Human pancreatic lipase-related protein 2 (PNLIPRP2)

Product Images

Western blot validation of overexpression lysate (Cat# LY401656) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC221745 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified PNLIPRP2 protein (Cat# TP321745). The protein was produced from HEK293T cells transfected with PNLIPRP2 cDNA clone (Cat# RC221745) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PNLIPRP2
• Synonyms: PLRP2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC221745 representing NM_005396
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGCTGCCCCCTTGGACCCTCGGCCTTCTCCTGCTGGCCACAGTCAGAGGAAAAGAGGTCTGCTACG
  GACAACTTGGCTGCTTTTCTGATGAAAAACCATGGGCAGGAACCCTTCAGCGACCTGTAAAATTACTTCC
  CTGGTCCCCCGAGGACATTGACACCCGCTTTCTTCTGTACACAAATGAAAATCCAAACAACTTCCAACTA
  ATCACTGGCACGGAACCAGACACCATTGAGGCTTCAAACTTCCAACTGGACCGCAAGACACGCTTCATCA
  TCCATGGCTTCTTAGACAAGGCGGAGGACAGCTGGCCATCGGACATGTGCAAGAAAATGTTTGAAGTGGA
  GAAGGTGAACTGCATCTGTGTGGACTGGAGGCACGGGTCCCGGGCAATGTACACCCAAGCCGTGCAAAAC
  ATTCGGGTTGTTGGGGCGGAGACAGCTTTCTTAATACAAGCACTGTCGACGCAGCTGGGGTACAGCCTTG
  AGGACGTGCATGTCATCGGCCACAGCCTGGGCGCGCACACGGCCGCGGAGGCGGGCAGGAGGCTGGGGGG
  CCGCGTGGGCAGGATCACAGGGCTGGATCCAGCAGGGCCGTGCTTCCAGGATGAACCTGAGGAGGTTCGG
  TTGGATCCATCTGACGCCGTGTTTGTGGATGTGATTCACACAGATTCTTCTCCCATAGTTCCTTCCCTAG
  GTTTCGGAATGAGCCAAAAGGTGGGCCATCTGGATTTCTTTCCAAATGGAGGAAAGGAAATGCCCGGATG
  TAAGAAAAATGTCCTTTCAACCATTACTGATATTGATGGAATATGGGAAGGAATTGGTGGCTTTGTGTCT
  TGCAATCACCTAAGAAGCTTCGAGTATTACTCAAGCAGCGTCCTCAACCCTGATGGCTTCCTGGGCTATC
  CCTGTGCCTCCTACGATGAGTTTCAGGAGAGTAAGTGTTTCCCTTGTCCAGCTGAAGGATGCCCCAAAAT
  GGGGCACTATGCTGACCAATTTAAGGGGAAAACAAGTGCTGTGGAACAAACCTTTTTCCTGAACACAGGA
  GAGAGTGGTAACTTTACTAGTTGGAGATATAAGGTATCAGTCACACTTTCTGGAAAAGAGAAAGTGAATG
  GGTACATCAGGATTGCTTTGTATGGAAGTAATGAAAACTCGAAACAATATGAGATTTTCAAAGGATCCCT
  CAAACCAGATGCAAGTCACACGTGTGCTATTGATGTGGATTTTAATGTTGGAAAAATACAGAAAGTTAAA
  TTCCTCTGGAACAAACGTGGGATAAATCTATCTGAGCCCAAACTGGGGGCTTCCCAAATCACAGTGCAAA
  GTGGTGAAGATGGGACTGAGTATAATTTTTGTAGCAGCGACACTGTGGAAGAAAACGTCTTGCAATCTCT
  TTACCCTTGT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC221745 representing NM_005396
  Red=Cloning site Green=Tags(s)
  
  MMLPPWTLGLLLLATVRGKEVCYGQLGCFSDEKPWAGTLQRPVKLLPWSPEDIDTRFLLYTNENPNNFQL
  ITGTEPDTIEASNFQLDRKTRFIIHGFLDKAEDSWPSDMCKKMFEVEKVNCICVDWRHGSRAMYTQAVQN
  IRVVGAETAFLIQALSTQLGYSLEDVHVIGHSLGAHTAAEAGRRLGGRVGRITGLDPAGPCFQDEPEEVR
  LDPSDAVFVDVIHTDSSPIVPSLGFGMSQKVGHLDFFPNGGKEMPGCKKNVLSTITDIDGIWEGIGGFVS
  CNHLRSFEYYSSSVLNPDGFLGYPCASYDEFQESKCFPCPAEGCPKMGHYADQFKGKTSAVEQTFFLNTG
  ESGNFTSWRYKVSVTLSGKEKVNGYIRIALYGSNENSKQYEIFKGSLKPDASHTCAIDVDFNVGKIQKVK
  FLWNKRGINLSEPKLGASQITVQSGEDGTEYNFCSSDTVEENVLQSLYPC
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005396
• ORF Size: 1410 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005396.1, NM_005396.2, NM_005396.3, NM_005396.4, NP_005387.1, NP_005387.2
• RefSeq Size: 1486 bp
• RefSeq ORF: 1410 bp
• Locus ID: 5408
• Cytogenetics: 10q25.3
• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: Glycerolipid metabolism, Metabolic pathways
• MW: 51.9 kDa
• Gene Summary: 'This gene encodes a lipase that hydrolyzes galactolipids, the main components of plant membrane lipids. An allelic polymorphism in this gene results in both coding and non-coding variants; the reference genome represents the non-coding allele. [provided by RefSeq, Aug 2015]'