SLC12A3 (NM_000339) Human Tagged ORF Clone
CAT#: RC221919L2
- LentiORF®
Lenti ORF clone of Human solute carrier family 12 (sodium/chloride transporters), member 3 (SLC12A3), transcript variant 1, mGFP tagged
"NM_000339" in other vectors (6)
Product Images
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Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | mGFP |
Symbol | SLC12A3 |
Synonyms | NCC; NCCT; TSC |
Vector | pLenti-C-mGFP |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
The ORF insert of this clone is exactly the same as(RC221919).
|
Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
ACCN | NM_000339 |
ORF Size | 3090 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_000339.1, NP_000330.1, NP_000330.2 |
RefSeq Size | 3131 bp |
RefSeq ORF | 3093 bp |
Locus ID | 6559 |
Cytogenetics | 16q13 |
Protein Families | Druggable Genome, Transmembrane |
MW | 113.7 kDa |
Gene Summary | 'This gene encodes a renal thiazide-sensitive sodium-chloride cotransporter that is important for electrolyte homeostasis. This cotransporter mediates sodium and chloride reabsorption in the distal convoluted tubule. Mutations in this gene cause Gitelman syndrome, a disease similar to Bartter's syndrome, that is characterized by hypokalemic alkalosis combined with hypomagnesemia, low urinary calcium, and increased renin activity associated with normal blood pressure. This cotransporter is the target for thiazide diuretics that are used for treating high blood pressure. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]' |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC300050 | SLC12A3 (untagged)-Human solute carrier family 12 (sodium/chloride transporters), member 3 (SLC12A3), transcript variant 1 |
USD 2,100.00 |
|
RC221919 | SLC12A3 (Myc-DDK-tagged)-Human solute carrier family 12 (sodium/chloride transporters), member 3 (SLC12A3), transcript variant 1 |
USD 1,080.00 |
|
RG221919 | SLC12A3 (GFP-tagged) - Human solute carrier family 12 (sodium/chloride transporters), member 3 (SLC12A3), transcript variant 1 |
USD 1,190.00 |
|
RC221919L1 | Lenti ORF clone of Human solute carrier family 12 (sodium/chloride transporters), member 3 (SLC12A3), transcript variant 1, Myc-DDK-tagged |
USD 1,584.00 |
|
RC221919L3 | Lenti ORF clone of Human solute carrier family 12 (sodium/chloride transporters), member 3 (SLC12A3), transcript variant 1, Myc-DDK-tagged |
USD 1,280.00 |
|
RC221919L4 | Lenti ORF clone of Human solute carrier family 12 (sodium/chloride transporters), member 3 (SLC12A3), transcript variant 1, mGFP tagged |
USD 1,280.00 |
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