RAPGEF2 (NM_014247) Human Tagged ORF Clone

CAT#: RC222055L4

Lenti-ORF clone of RAPGEF2 (mGFP-tagged)-Human Rap guanine nucleotide exchange factor (GEF) 2 (RAPGEF2)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: mGFP
• Symbol: RAPGEF2
• Synonyms: CNrasGEF; NRAPGEP; nRap GEP; PDZ-GEF1; PDZGEF1; RA-GEF; RA-GEF-1; RAGEF; Rap-GEP
• Vector: pLenti-C-mGFP-P2A-Puro
• E. coli Selection: Chloramphenicol (34 ug/mL)
• Mammalian Cell Selection: Puromycin
• Sequence Data:
  ORF Nucleotide Sequence

  The ORF insert of this clone is exactly the same as(RC222055).
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NM_014247
• ORF Size: 4497 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_014247.1, NP_055062.1
• RefSeq Size: 6568
• RefSeq ORF: 4500
• Locus ID: 9693
• Domains: RA, cNMP, RasGEFN, PDZ, RasGEF
• Protein Pathways: MAPK signaling pathway
• MW: 167.2 kDa
• Gene Summary: Members of the RAS (see HRAS; MIM 190020) subfamily of GTPases function in signal transduction as GTP/GDP-regulated switches that cycle between inactive GDP- and active GTP-bound states. Guanine nucleotide exchange factors (GEFs), such as RAPGEF2, serve as RAS activators by promoting acquisition of GTP to maintain the active GTP-bound state and are the key link between cell surface receptors and RAS activation (Rebhun et al., 2000 [PubMed 10934204]). [supplied by OMIM, Mar 2008]