ISM1 (NM_080826) Human Tagged ORF Clone

CAT#: RC222172

ISM1 (Myc-DDK-tagged)-Human isthmin 1 homolog (zebrafish) (ISM1)

Product Images

Western blot validation of overexpression lysate (Cat# LY409029) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC222172 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified ISM1 protein (Cat# TP322172). The protein was produced from HEK293T cells transfected with ISM1 cDNA clone (Cat# RC222172) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ISM1
• Synonyms: bA149I18.1; C20orf82; dJ1077I2.1; ISM; Isthmin
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC222172 representing NM_080826.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGTGCGCCTGGCGGCCGAGCTGCTGCTGCTGCTGGGGCTGCTGCTGCTCACGCTGCACATCACCGTG
  CTGCGCGGCTCGGGAGCCGCCGACGGGCCCGACGCGGCCGCGGGCAACGCCAGCCAAGCCCAGCTGCAG
  AATAACCTCAACGTGGGAAGTGACACCACATCAGAAACCAGCTTTTCTCTCTCCAAAGAAGCACCAAGG
  GAGCATCTGGACCACCAGGCTGCACACCAACCCTTCCCCAGACCGCGATTCCGACAAGAGACGGGGCAC
  CCTTCATTGCAAAGAGATTTCCCCAGATCCTTTCTCCTTGATCTACCAAACTTTCCAGATCTTTCCAAA
  GCTGATATCAATGGGCAGAATCCAAATATCCAGGTCACCATAGAGGTGGTCGACGGTCCTGACTCTGAA
  GCAGATAAAGATCAGCATCCGGAGAATAAGCCCAGCTGGTCAGTCCCATCCCCCGACTGGCGGGCCTGG
  TGGCAGAGGTCCCTGTCCTTGGCCAGGGCAAACAGCGGGGACCAGGACTACAAGTACGACAGTACCTCA
  GACGACAGCAACTTCCTCAACCCCCCCAGGGGGTGGGACCATACAGCCCCAGGCCACCGGACTTTTGAA
  ACCAAAGATCAGCCAGAATATGATTCCACAGATGGCGAGGGTGACTGGAGTCTCTGGTCTGTCTGCAGC
  GTCACCTGCGGGAACGGCAACCAGAAACGGACCCGGTCTTGTGGCTACGCGTGCACTGCAACAGAATCG
  AGGACCTGTGACCGTCCAAACTGCCCAGGAATTGAAGACACTTTTAGGACAGCTGCCACCGAAGTGAGT
  CTGCTTGCGGGAAGCGAGGAGTTTAATGCCACCAAACTGTTTGAAGTTGACACAGACAGCTGTGAGCGC
  TGGATGAGCTGCAAAAGCGAGTTCTTAAAGAAGTACATGCACAAGGTGATGAATGACCTGCCCAGCTGC
  CCCTGCTCCTACCCCACTGAGGTGGCCTACAGCACGGCCGACATCTTCGACCGCATCAAGCGCAAGGAC
  TTCCGCTGGAAGGACGCCAGCGGGCCCAAGGAGAAGCTGGAGATCTACAAGCCCACTGCCCGGTACTGC
  ATCCGCTCCATGCTGTCCCTGGAGAGCACCACGCTGGCGGCACAGCACTGCTGCTACGGCGACAACATG
  CAGCTCATCACCAGGGGCAAGGGGGCGGGCACGCCCAACCTCATCAGCACCGAGTTCTCCGCGGAGCTC
  CACTACAAGGTGGACGTCCTGCCCTGGATTATCTGCAAGGGTGACTGGAGCAGGTATAACGAGGCCCGG
  CCTCCCAACAACGGACAGAAGTGCACAGAGAGCCCCTCGGACGAGGACTACATCAAGCAGTTCCAAGAG
  GCCAGGGAATAT
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGAT
  ATCCTGGATTACAAGGATGACGACGATAAGGTTTAA
  
  Protein Sequence

  >Peptide sequence encoded by RC222172
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MVRLAAELLLLLGLLLLTLHITVLRGSGAADGPDAAAGNASQAQLQNNLNVGSDTTSETSFSLSKEAPR
  EHLDHQAAHQPFPRPRFRQETGHPSLQRDFPRSFLLDLPNFPDLSKADINGQNPNIQVTIEVVDGPDSE
  ADKDQHPENKPSWSVPSPDWRAWWQRSLSLARANSGDQDYKYDSTSDDSNFLNPPRGWDHTAPGHRTFE
  TKDQPEYDSTDGEGDWSLWSVCSVTCGNGNQKRTRSCGYACTATESRTCDRPNCPGIEDTFRTAATEVS
  LLAGSEEFNATKLFEVDTDSCERWMSCKSEFLKKYMHKVMNDLPSCPCSYPTEVAYSTADIFDRIKRKD
  FRWKDASGPKEKLEIYKPTARYCIRSMLSLESTTLAAQHCCYGDNMQLITRGKGAGTPNLISTEFSAEL
  HYKVDVLPWIICKGDWSRYNEARPPNNGQKCTESPSDEDYIKQFQEAREY
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
  
  Recombinant protein using RC222172 also available, TP322172M
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_080826
• ORF Size: 1392 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_080826.2
• RefSeq Size: 2610 bp
• RefSeq ORF: 1395 bp
• Locus ID: 140862
• UniProt ID: B1AKI9
• Cytogenetics: 20p12.1
• MW: 52.1 kDa
• Gene Summary: Acts as an angiogenesis inhibitor.[UniProtKB/Swiss-Prot Function]