DMP1 (NM_001079911) Human Tagged ORF Clone

CAT#: RC222208

DMP1 (Myc-DDK-tagged)-Human dentin matrix acidic phosphoprotein 1 (DMP1), transcript variant 2

Product Images

Coomassie blue staining of purified DMP1 protein (Cat# TP322208). The protein was produced from HEK293T cells transfected with DMP1 cDNA clone (Cat# RC222208) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: DMP1
• Synonyms: ARHP; ARHR; DMP-1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC222208 representing NM_001079911
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGATCAGCATCCTGCTCATGTTCCTTTGGGGATTATCCTGTGCTCTCCCAGTAACCAGGTATCAAA
  ATAATGAATCTGAGGATTCTGAAGAATGGAAGGGTCATTTGGCTCAGGCACCAACACCACCCTTGGCAAA
  TGAAGACCCCAGTGACTGCACTCAGTCAGAGGAGGGCCTGGGCTCTGATGATCATCAATACATTTATAGG
  CTAGCTGGTGGCTTCTCCAGGAGCACAGGAAAAGGAGGAGATGATAAAGATGACGATGAAGATGACAGTG
  GAGATGACACCTTTGGTGACGATGACAGTGGCCCAGGGCCCAAAGACAGACAAGAAGGAGGAAACTCCAG
  ACTGGGAAGTGATGAGGACTCTGATGACACCATACAAGCCAGTGAAGAGAGTGCCCCACAAGGGCAAGAC
  AGTGCCCAAGATACCACCAGTGAGAGCAGGGAACTTGACAATGAGGACCGGGTGGACAGCAAGCCTGAGG
  GAGGTGACTCCACTCAAGAGAGTGAGAGTGAAGAGCACTGGGTGGGAGGTGGCAGTGATGGGGAGAGCAG
  CCATGGAGACGGCTCCGAGTTGGACGATGAGGGAATGCAGAGTGATGACCCAGAGAGCATCAGGAGTGAA
  AGGGGAAACTCCAGAATGAACAGTGCAGGCATGAAATCAAAAGAATCTGGAGAAAACAGTGAGCAAGCAA
  ACACTCAAGATTCAGGTGGCAGCCAATTGCTGGAGCATCCCAGTAGGAAAATTTTTAGGAAGTCTCGCAT
  CTCAGAGGAAGATGACAGAAGCGAGCTTGATGACAACAACACAATGGAAGAAGTCAAGAGTGACTCTACA
  GAAAACAGCAACTCCAGAGACACTGGCCTCAGCCAACCCAGGAGAGACAGCAAGGGTGACTCTCAAGAAG
  ACAGCAAGGAGAATCTGTCCCAGGAAGAGAGCCAAAACGTAGATGGTCCCAGCAGTGAGTCCAGCCAAGA
  GGCCAACCTGTCATCTCAAGAGAACAGCAGTGAGTCTCAGGAAGAGGTGGTGAGTGAGTCCAGGGGAGAT
  AACCCCGACCCCACAACTAGTTATGTAGAAGACCAGGAAGACAGTGACTCCAGCGAGGAGGACAGCTCGC
  ACACACTCTCCCACTCAAAAAGTGAATCCAGAGAGGAGCAAGCAGACAGCGAATCCAGTGAGAGCCTCAA
  CTTCTCAGAGGAAAGCCCGGAGTCCCCTGAGGATGAGAACAGCTCCAGCCAGGAGGGCCTCCAGTCTCAC
  AGCAGCTCAGCAGAGAGTCAGAGCGAGGAAAGCCATTCTGAGGAAGACGACAGTGACTCTCAAGACAGCA
  GCAGATCCAAAGAAGATAGCAACTCCACGGAGAGCAAATCAAGCAGTGAGGAAGATGGCCAGTTGAAAAA
  CATTGAGATAGAGAGCCGGAAATTAACAGTTGATGCCTATCACAACAAACCCATTGGGGACCAAGATGAC
  AATGACTGCCAAGACGGCTAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC222208 representing NM_001079911
  Red=Cloning site Green=Tags(s)
  
  MKISILLMFLWGLSCALPVTRYQNNESEDSEEWKGHLAQAPTPPLANEDPSDCTQSEEGLGSDDHQYIYR
  LAGGFSRSTGKGGDDKDDDEDDSGDDTFGDDDSGPGPKDRQEGGNSRLGSDEDSDDTIQASEESAPQGQD
  SAQDTTSESRELDNEDRVDSKPEGGDSTQESESEEHWVGGGSDGESSHGDGSELDDEGMQSDDPESIRSE
  RGNSRMNSAGMKSKESGENSEQANTQDSGGSQLLEHPSRKIFRKSRISEEDDRSELDDNNTMEEVKSDST
  ENSNSRDTGLSQPRRDSKGDSQEDSKENLSQEESQNVDGPSSESSQEANLSSQENSSESQEEVVSESRGD
  NPDPTTSYVEDQEDSDSSEEDSSHTLSHSKSESREEQADSESSESLNFSEESPESPEDENSSSQEGLQSH
  SSSAESQSEESHSEEDDSDSQDSSRSKEDSNSTESKSSSEEDGQLKNIEIESRKLTVDAYHNKPIGDQDD
  NDCQDGY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001079911
• ORF Size: 1491 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001079911.1, NM_001079911.2, NP_001073380.1
• RefSeq Size: 2631 bp
• RefSeq ORF: 1494 bp
• Locus ID: 1758
• Protein Families: Secreted Protein
• MW: 54.13 kDa
• Gene Summary: Dentin matrix acidic phosphoprotein is an extracellular matrix protein and a member of the small integrin binding ligand N-linked glycoprotein family. This protein, which is critical for proper mineralization of bone and dentin, is present in diverse cells of bone and tooth tissues. The protein contains a large number of acidic domains, multiple phosphorylation sites, a functional arg-gly-asp cell attachment sequence, and a DNA binding domain. In undifferentiated osteoblasts it is primarily a nuclear protein that regulates the expression of osteoblast-specific genes. During osteoblast maturation the protein becomes phosphorylated and is exported to the extracellular matrix, where it orchestrates mineralized matrix formation. Mutations in the gene are known to cause autosomal recessive hypophosphatemia, a disease that manifests as rickets and osteomalacia. The gene structure is conserved in mammals. Two transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2008]