OR10T2 (NM_001004475) Human Tagged ORF Clone

CAT#: RC222225

OR10T2 (Myc-DDK-tagged)-Human olfactory receptor, family 10, subfamily T, member 2 (OR10T2)

Product Images

Western blot validation of overexpression lysate (Cat# LY423778) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC222225 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: OR10T2
• Synonyms: OR1-3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC222225 representing NM_001004475
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGAGGTTTCAACAAAACCACTGTGGTTACACAGTTCATCCTGGTGGGTTTCTCCAGCCTGGGGGAGC
  TCCAGCTGCTGCTTTTTGTCATCTTTCTTCTCCTATACTTGACAATCCTGGTGGCCAATGTGACCATCAT
  GGCCGTTATTCGCTTCAGCTGGACTCTCCACACTCCCATGTATGGCTTTCTATTCATCCTTTCATTTTCT
  GAGTCCTGCTACACTTTTGTCATCATCCCTCAGCTGCTGGTCCACCTGCTCTCAGACACCAAGACCATCT
  CCTTCATGGCCTGTGCCACCCAGCTGTTCTTTTTCCTTGGCTTTGCTTGCACCAACTGCCTCCTCATTGC
  TGTGATGGGATATGATCGCTATGTAGCAATTTGTCACCCTCTGAGGTACACACTCATCATAAACAAAAGG
  CTGGGGTTGGAGTTGATTTCTCTCTCAGGAGCCACAGGTTTCTTTATTGCTTTGGTGGCCACCAACCTCA
  TTTGTGACATGCGTTTTTGTGGCCCCAACAGGGTTAACCACTATTTCTGTGACATGGCACCTGTTATCAA
  GTTAGCCTGCACTGACACCCATGTGAAAGAGCTGGCTTTATTTAGCCTCAGCATCCTGGTAATTATGGTG
  CCTTTTCTGTTAATTCTCATATCCTATGGCTTCATAGTTAACACCATCCTGAAGATCCCCTCAGCTGAGG
  GCAAGAAGGCCTTTGTCACCTGTGCCTCACATCTCACTGTGGTCTTTGTCCACTATGGCTGTGCCTCTAT
  CATCTATCTGCGGCCCAAGTCCAAGTCTGCCTCAGACAAGGATCAGTTGGTGGCAGTGACCTACACAGTG
  GTTACTCCCTTACTTAATCCTCTTGTCTACAGTCTGAGGAACAAAGAGGTAAAAACTGCATTGAAAAGAG
  TTCTTGGAATGCCTGTGGCAACCAAGATGAGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC222225 representing NM_001004475
  Red=Cloning site Green=Tags(s)
  
  MRGFNKTTVVTQFILVGFSSLGELQLLLFVIFLLLYLTILVANVTIMAVIRFSWTLHTPMYGFLFILSFS
  ESCYTFVIIPQLLVHLLSDTKTISFMACATQLFFFLGFACTNCLLIAVMGYDRYVAICHPLRYTLIINKR
  LGLELISLSGATGFFIALVATNLICDMRFCGPNRVNHYFCDMAPVIKLACTDTHVKELALFSLSILVIMV
  PFLLILISYGFIVNTILKIPSAEGKKAFVTCASHLTVVFVHYGCASIIYLRPKSKSASDKDQLVAVTYTV
  VTPLLNPLVYSLRNKEVKTALKRVLGMPVATKMS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001004475
• ORF Size: 942 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001004475.1, NP_001004475.1
• RefSeq Size: 945
• RefSeq ORF: 945
• Locus ID: 128360
• Protein Families: Transmembrane
• Protein Pathways: Olfactory transduction
• MW: 34.8 kDa
• Gene Summary: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]