HLTF (NM_139048) Human Tagged ORF Clone
CAT#: RC222346
HLTF (Myc-DDK-tagged)-Human helicase-like transcription factor (HLTF), transcript variant 2
"NM_139048" in other vectors (4)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | HLTF |
Synonyms | HIP116; HIP116A; HLTF1; RNF80; SMARCA3; SNF2L3; ZBU1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC222346 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCTGGATGTTCAAGAGGGATCCAGTTTGGAAGTACTTGCAGACTGTCCAGTATGGAGTTCATGGAA ATTTTCCACGCCTCTCATATCCAACTTTCTTTCCACGTTTTGAATTCCAAGATGTTATCCCTCCAGATGA CTTTCTAACTAGTGATGAAGAAGTAGATTCCGTTTTATTTGGAAGTTTGAGAGGTCATGTGGTTGGACTA CGCTATTACACGGGAGTAGTTAATAATAATGAAATGGTTGCATTACAACGAGATCCTAATAACCCTTATG ATAAGAATGCAATTAAAGTAAACAATGTGAATGGAAATCAAGTTGGCCATTTAAAGAAAGAGCTTGCAGG TGCTTTGGCCTATATCATGGACAACAAATTGGCACAAATTGAAGGGGTAGTTCCTTTTGGTGCAAACAAT GCTTTTACCATGCCTCTGCATATGACTTTTTGGGGAAAAGAAGAAAATAGAAAAGCGGTTTCAGATCAGT TGAAGAAACATGGATTTAAATTGGGTCCTGCACCAAAAACTTTAGGATTCAATTTGGAAAGTGGTTGGGG CTCTGGAAGAGCTGGACCAAGCTATAGTATGCCAGTGCATGCTGCAGTACAGATGACAACTGAACAGCTT AAAACAGAATTTGACAAATTGTTTGAAGATTTAAAAGAAGATGATAAAACCCATGAAATGGAACCAGCTG AGGCTATTGAAACACCACTGCTTCCACATCAAAAACAAGCTCTAGCTTGGATGGTGTCACGGGAAAATAG CAAAGAACTTCCACCATTCTGGGAACAGCGAAATGACTTATACTATAACACAATAACAAATTTTTCTGAG AAGGACCGACCAGAAAATGTCCATGGAGGAATTTTAGCTGATGATATGGGTTTGGGTAAAACTCTTACAG CCATTGCAGTAATCCTTACCAACTTCCATGATGGCAGACCTCTTCCTATTGAAAGAGTTAAAAAGAATCT ACTGAAGAAGGAATATAATGTTAACGATGACTCTATGAAACTTGGAGGAAACAATACCAGTGAAAAGGCA GATGGACTAAGCAAAGACGCATCTAGATGTAGTGAACAACCCAGTATTTCAGATATCAAGGAGAAGAGTA AGTTTCGCATGTCAGAATTGTCTAGCTCCCGCCCCAAAAGAAGAAAAACTGCTGTCCAGTACATAGAAAG CAGTGATTCAGAGGAAATTGAAACAAGTGAATTGCCGCAGAAAATGAAAGGCAAACTGAAAAATGTACAG TCTGAAACTAAAGGCAGGGCGAAAGGATCTTCTAAGGTTATAGAAGATGTGGCATTTGCATGTGCATTAA CTTCATCTGTTCCTACAACAAAAAAGAAAATGTTGAAAAAGGGAGCTTGTGCAGTGGAGGGGTCAAAGAA AACTGATGTTGAGGAGAGACCAAGAACAACACTGATCATCTGTCCGCTTTCTGTGTTAAGCAACTGGATT GACCAGTTTGGACAACATATAAAATCAGATGTACACTTGAATTTTTATGTTTATTATGGTCCTGATCGTA TTAGAGAACCGGCCTTACTTTCAAAACAGGATATTGTTTTGACTACGTATAATATTTTAACTCATGACTA TGGAACTAAAGGAGATAGTCCATTACATAGCATAAGGTGGCTAAGAGTGATCCTGGATGAAGGACATGCC ATACGAAATCCAAATGCTCAGCAGACAAAAGCTGTACTTGACTTAGAATCAGAAAGAAGATGGGTTTTGA CAGGTACTCCAATCCAGAATTCTTTAAAGGACTTGTGGTCTCTTCTTTCCTTTTTAAAACTTAAACCATT TATTGATAGAGAATGGTGGCATAGAACAATACAGCGTCCTGTCACAATGGGAGATGAAGGAGGACTTAGG CGTTTACAGTCCCTAATTAAAAATATTACACTTAGAAGAACAAAGACAAGCAAAATTAAAGGAAAACCTG TTTTGGAGTTACCAGAACGTAAAGTATTTATTCAGCACATTACACTTTCAGATGAAGAGAGAAAGATTTA TCAGTCTGTGAAAAATGAAGGCAGAGCCACTATTGGAAGGTATTTTAATGAAGGGACTGTCCTGGCACAT TATGCAGATGTCCTGGGTCTTTTGCTTAGACTGCGGCAAATTTGTTGCCATACTTACCTTCTTACAAATG CAGTGTCTTCCAATGGCCCCTCAGGAAATGATACACCTGAAGAACTGAGAAAGAAGTTAATAAGGAAGAT GAAGTTAATTCTGAGCTCAGGTTCAGATGAGGAATGTGCAATTTGCCTGGATTCTTTAACAGTTCCTGTG ATAACACATTGTGCACATGTATTTTGTAAACCCTGTATTTGCCAAGTCATTCAGAATGAGCAGCCACATG CTAAATGCCCTTTATGCAGAAATGATATACATGAAGATAATTTATTAGAATGTCCTCCAGAAGAATTAGC ACGTGACAGTGAGAAAAAGTCTGATATGGAATGGACATCCAGTTCAAAGATTAATGCGCTAATGCACGCA TTGACTGACTTAAGAAAGAAGAATCCCAACATAAAAAGTTTGGTTGTTTCTCAGTTTACAACATTCCTGT CTTTAATAGAAATACCACTTAAAGCCTCTGGATTTGTGTTTACTCGTTTGGATGGTTCCATGGCCCAAAA GAAAAGAGTTGAATCAATTCAGTGTTTTCAAAACACTGAAGCAGGATCTCCAACTATAATGCTTCTGTCC TTAAAAGCAGGTGGAGTTGGTTTGAATCTGTCTGCAGCTTCTCGAGTGTTTTTAATGGATCCAGCCTGGA ATCCTGCTGCTGAAGATCAGTGCTTTGACAGATGCCATAGACTTGGTCAGAAGCAAGAAGTTATCATCAC AAAATTCATTGTAAAGGACTCTGTTGAAGAAAATATGCTGAAAATACAAAACAAAAAGAGAGAACTTGCA GCAGGAGCCTTTGGAACTAAAAAACCAAATGCTGACGAAATGAAACAAGCCAAAATTAATGAAATCAGAA CATTAATTGACTTA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC222346 protein sequence
Red=Cloning site Green=Tags(s) MSWMFKRDPVWKYLQTVQYGVHGNFPRLSYPTFFPRFEFQDVIPPDDFLTSDEEVDSVLFGSLRGHVVGL RYYTGVVNNNEMVALQRDPNNPYDKNAIKVNNVNGNQVGHLKKELAGALAYIMDNKLAQIEGVVPFGANN AFTMPLHMTFWGKEENRKAVSDQLKKHGFKLGPAPKTLGFNLESGWGSGRAGPSYSMPVHAAVQMTTEQL KTEFDKLFEDLKEDDKTHEMEPAEAIETPLLPHQKQALAWMVSRENSKELPPFWEQRNDLYYNTITNFSE KDRPENVHGGILADDMGLGKTLTAIAVILTNFHDGRPLPIERVKKNLLKKEYNVNDDSMKLGGNNTSEKA DGLSKDASRCSEQPSISDIKEKSKFRMSELSSSRPKRRKTAVQYIESSDSEEIETSELPQKMKGKLKNVQ SETKGRAKGSSKVIEDVAFACALTSSVPTTKKKMLKKGACAVEGSKKTDVEERPRTTLIICPLSVLSNWI DQFGQHIKSDVHLNFYVYYGPDRIREPALLSKQDIVLTTYNILTHDYGTKGDSPLHSIRWLRVILDEGHA IRNPNAQQTKAVLDLESERRWVLTGTPIQNSLKDLWSLLSFLKLKPFIDREWWHRTIQRPVTMGDEGGLR RLQSLIKNITLRRTKTSKIKGKPVLELPERKVFIQHITLSDEERKIYQSVKNEGRATIGRYFNEGTVLAH YADVLGLLLRLRQICCHTYLLTNAVSSNGPSGNDTPEELRKKLIRKMKLILSSGSDEECAICLDSLTVPV ITHCAHVFCKPCICQVIQNEQPHAKCPLCRNDIHEDNLLECPPEELARDSEKKSDMEWTSSSKINALMHA LTDLRKKNPNIKSLVVSQFTTFLSLIEIPLKASGFVFTRLDGSMAQKKRVESIQCFQNTEAGSPTIMLLS LKAGGVGLNLSAASRVFLMDPAWNPAAEDQCFDRCHRLGQKQEVIITKFIVKDSVEENMLKIQNKKRELA AGAFGTKKPNADEMKQAKINEIRTLIDL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_139048 |
ORF Size | 443 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_139048.1, NM_139048.2, NP_620636.1 |
RefSeq Size | 4514 bp |
RefSeq ORF | 3030 bp |
Locus ID | 6596 |
Protein Families | Druggable Genome, Transcription Factors |
MW | 113.9 kDa |
Gene Summary | This gene encodes a member of the SWI/SNF family. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein contains a RING finger DNA binding motif. Two transcript variants encoding the same protein have been found for this gene. However, use of an alternative translation start site produces an isoform that is truncated at the N-terminus compared to the full-length protein. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC109776 | HLTF (untagged)-Human helicase-like transcription factor (HLTF), transcript variant 2 |
USD 310.00 |
|
RG222346 | HLTF (GFP-tagged) - Human helicase-like transcription factor (HLTF), transcript variant 2 |
USD 970.00 |
|
RC222346L3 | Lenti ORF clone of Human helicase-like transcription factor (HLTF), transcript variant 2, Myc-DDK-tagged |
USD 1,080.00 |
|
RC222346L4 | Lenti ORF clone of Human helicase-like transcription factor (HLTF), transcript variant 2, mGFP tagged |
USD 1,080.00 |
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