Synphilin 1 (SNCAIP) (NM_005460) Human Tagged ORF Clone

CAT#: RC222425

SNCAIP (Myc-DDK-tagged)-Human synuclein, alpha interacting protein (SNCAIP)

Product Images

Coomassie blue staining of purified SNCAIP protein (Cat# TP322425). The protein was produced from HEK293T cells transfected with SNCAIP cDNA clone (Cat# RC222425) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SNCAIP
• Synonyms: Sph1; SYPH1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC222425 representing NM_005460
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAGCCCCTGAATACCTTGATTTGGATGAAATTGACTTTAGTGATGACATATCTTATTCAGTCACAT
  CACTCAAGACGATCCCAGAACTGTGCCGAAGATGTGATACGCAAAACGAAGACAGATCAGTTTCTAGCTC
  TAGCTGGAATTGTGGCATCTCAACTCTTATTACAAACACGCAAAAGCCCACAGGAATCGCTGATGTGTAC
  AGTAAGTTCCGCCCAGTGAAGCGGGTTTCGCCACTGAAACATCAGCCAGAGACTCTGGAGAACAATGAAA
  GTGATGACCAAAAGAACCAGAAAGTGGTTGAGTACCAGAAAGGGGGTGAGTCTGACCTGGGCCCCCAGCC
  TCAGGAGCTTGGCCCTGGAGATGGAGTGGGCGGCCCACCAGGTAAGAGCTCTGAGCCCAGCACATCGCTG
  GGTGAACTGGAGCACTACGACCTCGACATGGATGAGATTCTGGATGTGCCTTATATTAAATCCAGTCAGC
  AGCTTGCCTCTTTTACCAAGGTGACTTCAGAAAAAAGAATTTTGGGCTTATGCACAACCATCAATGGCCT
  TTCTGGCAAAGCCTGCTCTACAGGAAGTTCTGAGAGCTCATCATCCAACATGGCACCATTTTGTGTTCTT
  TCTCCCGTGAAAAGCCCTCACTTGAGAAAAGCATCAGCTGTCATCCACGACCAGCACAAGCTGTCCACTG
  AAGAAACCGAGATCTCACCTCCTCTGGTTAAATGTGGCTCTGCATATGAGCCTGAAAACCAGAGTAAAGA
  CTTCCTAAACAAGACATTTAGTGATCCTCATGGTCGAAAAGTTGAGAAGACAACACCAGACTGCCAGCTC
  AGGGCCTTCCACCTACAATCCTCAGCAGCAGAATCCAAACCAGAAGAGCAGGTCAGTGGCCTAAACCGGA
  CCAGCTCCCAAGGCCCAGAAGAAAGGAGTGAGTATCTGAAAAAAGTGAAAAGCATCTTGAACATTGTTAA
  AGAAGGACAGATCTCTCTCCTGCCACACCTAGCTGCAGACAATCTAGACAAAATTCACGACGAAAATGGA
  AACAATCTATTACATATTGCGGCGTCACAGGGACACGCAGAGTGTCTACAGCACCTCACTTCTTTGATGG
  GAGAAGACTGCCTCAATGAGCGCAACACTGAGAAGTTGACTCCAGCAGGCCTGGCCATTAAGAATGGTCA
  GTTGGAGTGCGTACGCTGGATGGTGAGCGAAACAGAAGCCATTGCAGAACTGAGTTGTTCTAAGGATTTT
  CCAAGCCTTATTCATTACGCAGGTTGCTATGGCCAGGAAAAGATTCTTCTGTGGCTTCTTCAGTTTATGC
  AAGAACAGGGCATCTCGTTGGATGAAGTAGACCAGGATGGCAACAGTGCCGTTCACGTAGCCTCACAGCA
  TGGCTACCTTGGATGCATACAGACCTTGGTTGAATATGGAGCAAATGTCACCATGCAGAACCACGCTGGG
  GAAAAGCCCTCCCAGAGCGCCGAGCGGCAGGGGCACACCCTGTGCTCCAGGTACCTGGTGGTGGTGGAGA
  CCTGCATGTCGCTGGCCTCTCAAGTGGTGAAGTTAACCAAGCAGCTAAAGGAACAAACAGTAGAACGTGT
  CACGCTGCAGAACCAACTCCAACAATTTCTAGAAGCCCAGAAATCAGAGGGCAAGTCACTCCCTTCTTCA
  CCCAGTTCACCATCCTCACCTGCCTCCAGAAAGTCCCAGTGGAAATCTCCAGATGCAGATGATGATTCTG
  TAGCCAAAAGCAAGCCAGGAGTCCAAGAGGGGATTCAGGTTCTTGGAAGCCTGTCAGCCTCCAGCCGGGC
  TAGACCCAAAGCAAAAGATGAAGATTCTGATAAAATCTTACGCCAGTTATTGGGAAAGGAAATCTCAGAA
  AATGTCTGCACCCAGGAAAAACTGTCCTTGGAATTCCAGGATGCTCAGGCTTCCTCTAGAAATTCTAAAA
  AGATCCCACTGGAGAAGAGGGAACTGAAGTTAGCCAGGCTGAGACAGCTGATGCAGAGGTCACTGAGTGA
  GTCTGACACAGACTCCAACAACTCTGAGGACCCCAAGACTACCCCAGTGAGGAAGGCTGACCGACCAAGG
  CCGCAGCCCATTGTAGAAAGCGTAGAGAGTATGGACAGCGCAGAAAGCCTGCACCTGATGATTAAGAAAC
  ACACCTTGGCATCAGGGGGACGCAGGTTTCCTTTCAGCATCAAGGCCTCCAAATCCCTGGATGGCCACAG
  CCCATCTCCCACCTCAGAGAGCAGCGAACCAGACTTAGAATCCCAGTATCCAGGCTCAGGGAGTATTCCT
  CCAAACCAGCCCTCTGGTGACCCTCAGCAGCCCAGCCCTGACAGTACTGCTGCCCAGAAAGTTGCCACAA
  GTCCCAAGAGTGCCCTCAAGTCTCCATCTTCCAAGCGTAGGACATCTCAGAACTTAAAACTGAGAGTTAC
  CTTTGAGGAGCCTGTGGTGCAGATGGAGCAGCCTAGCCTTGAACTGAATGGAGAAAAAGACAAAGATAAG
  GGCAGGACTCTCCAGCGGACCTCCACAAGTAACGAATCGGGGGATCAACTGAAAAGGCCTTTTGGAGCCT
  TTCGATCTATCATGGAGACACTAAGTGGCAACCAAAACAATAATAATAACTACCAGGCAGCCAACCAGCT
  GAAAACCTCTACATTGCCCTTGACCTCACTTGGGAGGAAGACAGATGCCAAGGGAAACCCTGCCAGCTCC
  GCTAGCAAAGGAAAGAATAAGGCAGCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC222425 representing NM_005460
  Red=Cloning site Green=Tags(s)
  
  MEAPEYLDLDEIDFSDDISYSVTSLKTIPELCRRCDTQNEDRSVSSSSWNCGISTLITNTQKPTGIADVY
  SKFRPVKRVSPLKHQPETLENNESDDQKNQKVVEYQKGGESDLGPQPQELGPGDGVGGPPGKSSEPSTSL
  GELEHYDLDMDEILDVPYIKSSQQLASFTKVTSEKRILGLCTTINGLSGKACSTGSSESSSSNMAPFCVL
  SPVKSPHLRKASAVIHDQHKLSTEETEISPPLVKCGSAYEPENQSKDFLNKTFSDPHGRKVEKTTPDCQL
  RAFHLQSSAAESKPEEQVSGLNRTSSQGPEERSEYLKKVKSILNIVKEGQISLLPHLAADNLDKIHDENG
  NNLLHIAASQGHAECLQHLTSLMGEDCLNERNTEKLTPAGLAIKNGQLECVRWMVSETEAIAELSCSKDF
  PSLIHYAGCYGQEKILLWLLQFMQEQGISLDEVDQDGNSAVHVASQHGYLGCIQTLVEYGANVTMQNHAG
  EKPSQSAERQGHTLCSRYLVVVETCMSLASQVVKLTKQLKEQTVERVTLQNQLQQFLEAQKSEGKSLPSS
  PSSPSSPASRKSQWKSPDADDDSVAKSKPGVQEGIQVLGSLSASSRARPKAKDEDSDKILRQLLGKEISE
  NVCTQEKLSLEFQDAQASSRNSKKIPLEKRELKLARLRQLMQRSLSESDTDSNNSEDPKTTPVRKADRPR
  PQPIVESVESMDSAESLHLMIKKHTLASGGRRFPFSIKASKSLDGHSPSPTSESSEPDLESQYPGSGSIP
  PNQPSGDPQQPSPDSTAAQKVATSPKSALKSPSSKRRTSQNLKLRVTFEEPVVQMEQPSLELNGEKDKDK
  GRTLQRTSTSNESGDQLKRPFGAFRSIMETLSGNQNNNNNYQAANQLKTSTLPLTSLGRKTDAKGNPASS
  ASKGKNKAA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005460
• ORF Size: 2757 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005460.1, NM_005460.2, NM_005460.3, NP_005451.1, NP_005451.2
• RefSeq Size: 3569
• RefSeq ORF: 2760
• Locus ID: 9627
• Domains: ANK
• Protein Pathways: Parkinson's disease
• MW: 100.9 kDa
• Gene Summary: This gene encodes a protein containing several protein-protein interaction domains, including ankyrin-like repeats, a coiled-coil domain, and an ATP/GTP-binding motif. The encoded protein interacts with alpha-synuclein in neuronal tissue and may play a role in the formation of cytoplasmic inclusions and neurodegeneration. A mutation in this gene has been associated with Parkinson's disease. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2015]