ACRV1 (NM_020107) Human Tagged ORF Clone

CAT#: RC222483

ACRV1 (Myc-DDK-tagged)-Human acrosomal vesicle protein 1 (ACRV1), transcript variant 3

Product Images

Western blot validation of overexpression lysate (Cat# LY412644) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC222483 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified ACRV1 protein (Cat# TP322483). The protein was produced from HEK293T cells transfected with ACRV1 cDNA clone (Cat# RC222483) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ACRV1
• Synonyms: D11S4365; SP-10; SPACA2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC222483 representing NM_020107
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACAGGTTTCTCTTGCTAATGAGTCTTTATCTGCTTGGATCTGCCAGAGGAACATCAAGTCAGCCTA
  ATGAGCTTTCTGGCTCCATAGATCATCAAACTTCAGTTCAGCAACTTCCAGGTGAGCCCGCTGCGACTGA
  ACATGCTGAAGGTGAGCATACTGTAGGTGAGCAGCCTTCAGGAGAACAGCCTTCAGGTGAACACCTCTCC
  GGAGAACAGCCTTTGAGTGAGCTTGAGTCAGGTGAACAGCCTTCAGATGAACAGCCTTCAGGTGAACATG
  GCTCCGGTGAACAGCCTTCTGGTGAGCAGGCCTCGGGTGAACAGCCTTCAGGTGAGCACGCTTCAGGGGA
  ACAGGCTTCAGGTGCACCAATTTCAAGCACATCTACAGGCACAATATTAAATTGCTACACATGTGCTTAT
  ATGAATGATCAAGGAAAATGTCTTCGTGGAGAGGGAACCTGCATCACTCAGAATTCCCAGCAGTGCATGT
  TAAAGAAGATCTTTGAAGGTGGAAAACTCCAATTCATGGTTCAAGGGTGTGAGAACATGTGCCCATCTAT
  GAACCTCTTCTCCCATGGAACGAGGATGCAAATTATATGCTGTCGAAATCAATCTTTCTGCAATAAGATC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC222483 representing NM_020107
  Red=Cloning site Green=Tags(s)
  
  MNRFLLLMSLYLLGSARGTSSQPNELSGSIDHQTSVQQLPGEPAATEHAEGEHTVGEQPSGEQPSGEHLS
  GEQPLSELESGEQPSDEQPSGEHGSGEQPSGEQASGEQPSGEHASGEQASGAPISSTSTGTILNCYTCAY
  MNDQGKCLRGEGTCITQNSQQCMLKKIFEGGKLQFMVQGCENMCPSMNLFSHGTRMQIICCRNQSFCNKI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_020107
• ORF Size: 630 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020107.1, NM_020107.2, NM_020107.3, NM_020107.4, NP_064492.1
• RefSeq Size: 1173 bp
• RefSeq ORF: 633 bp
• Locus ID: 56
• Protein Families: Druggable Genome
• MW: 20 kDa
• Gene Summary: This gene encodes a testis-specific, differentiation antigen, acrosomal vesicle protein 1, that arises within the acrosomal vesicle during spermatogenesis, and is associated with the acrosomal membranes and matrix of mature sperm. The acrosomal vesicle protein 1 may be involved in sperm-zona binding or penetration. Alternatively spliced transcript variants have been described. [provided by RefSeq, Nov 2010]