IFNW1 (NM_002177) Human Tagged ORF Clone

CAT#: RC222484

IFNW1 (Myc-DDK-tagged)-Human interferon, omega 1 (IFNW1)

Product Images

Western blot validation of overexpression lysate (Cat# LY419484) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC222484 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: IFNW1
• Synonyms: IFN-omega 1, interferon omega-1; interferon, omega 1; interferon omega-1; OTTHUMP00000021133
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC222484 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCTCCTGTTCCCTCTACTGGCAGCCCTAGTGATGACCAGCTATAGCCCTGTTGGATCTCTGGGCT
  GTGATCTGCCTCAGAACCATGGCCTACTTAGCAGGAACACCTTGGTGCTTCTGCACCAAATGAGGAGAAT
  CTCCCCTTTCTTGTGTCTCAAGGACAGAAGAGACTTCAGGTTCCCCCAGGAGATGGTAAAAGGGAGCCAG
  TTGCAGAAGGCCCATGTCATGTCTGTCCTCCATGAGATGCTGCAGCAGATCTTCAGCCTCTTCCACACAG
  AGCGCTCCTCTGCTGCCTGGAACATGACCCTCCTAGACCAACTCCACACTGGACTTCATCAGCAACTGCA
  ACACCTGGAGACCTGCTTGCTGCAGGTAGTGGGAGAAGGAGAATCTGCTGGGGCAATTAGCAGCCCTGCA
  CTGACCTTGAGGAGGTACTTCCAGGGAATCCGTGTCTACCTGAAAGAGAAGAAATACAGCGACTGTGCCT
  GGGAAGTTGTCAGAATGGAAATCATGAAATCCTTGTTCTTATCAACAAACATGCAAGAAAGACTGAGAAG
  TAAAGATAGAGACCTGGGCTCATCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC222484 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MALLFPLLAALVMTSYSPVGSLGCDLPQNHGLLSRNTLVLLHQMRRISPFLCLKDRRDFRFPQEMVKGSQ
  LQKAHVMSVLHEMLQQIFSLFHTERSSAAWNMTLLDQLHTGLHQQLQHLETCLLQVVGEGESAGAISSPA
  LTLRRYFQGIRVYLKEKKYSDCAWEVVRMEIMKSLFLSTNMQERLRSKDRDLGSS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002177
• ORF Size: 585 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002177.1, NM_002177.2, NP_002168.1
• RefSeq Size: 1514 bp
• RefSeq ORF: 588 bp
• Locus ID: 3467
• Cytogenetics: 9p21.3
• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: Cytokine-cytokine receptor interaction, Jak-STAT signaling pathway, RIG-I-like receptor signaling pathway
• MW: 22.3 kDa
• Gene Summary: 'The protein encoded by this gene is an interferon and possesses antiviral activity. The encoded protein binds to the interferon alpha/beta receptor but not to the interferon gamma receptor. This intronless gene has several pseudogenes spread throughout the genome. [provided by RefSeq, Nov 2015]'