MGAT2 (NM_002408) Human Tagged ORF Clone

CAT#: RC222495

MGAT2 (Myc-DDK-tagged)-Human mannosyl (alpha-1,6-)-glycoprotein beta-1,2-N-acetylglucosaminyltransferase (MGAT2)

Product Images

Western blot validation of overexpression lysate (Cat# LY419342) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC222495 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: MGAT2
• Synonyms: CDG2A; CDGS2; GLCNACTII; GNT-II; GNT2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC222495 representing NM_002408
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGTTCCGCATCTACAAACGGAAGGTGCTAATCCTGACGCTCGTGGTGGCCGCCTGCGGCTTCGTCC
  TCTGGAGCAGCAATGGGCGACAAAGGAAGAACGAGGCCCTCGCCCCACCGTTGCTGGACGCCGAACCCGC
  GCGGGGTGCCGGCGGCCGCGGTGGGGACCACCCCTCTGTGGCTGTGGGCATCCGCAGGGTCTCCAACGTG
  TCGGCGGCTTCCCTGGTCCCGGCGGTCCCCCAGCCCGAGGCGGACAACCTGACGCTGCGGTACCGGTCCC
  TGGTGTACCAGCTGAACTTTGATCAGACCCTGAGGAATGTAGATAAGGCTGGCACCTGGGCCCCCCGGGA
  GCTGGTGCTGGTGGTCCAGGTGCATAACCGGCCCGAATACCTCAGACTGCTGCTGGACTCACTTCGAAAA
  GCCCAGGGAATTGACAACGTCCTCGTCATCTTTAGCCATGACTTCTGGTCGACCGAGATCAATCAGCTGA
  TCGCCGGGGTGAATTTCTGTCCGGTTCTGCAGGTGTTCTTTCCTTTCAGCATTCAGTTGTACCCTAACGA
  GTTTCCAGGTAGTGACCCTAGAGATTGTCCCAGAGACCTGCCGAAGAATGCCGCTTTGAAATTGGGGTGC
  ATCAATGCTGAGTATCCCGACTCCTTCGGCCATTATAGAGAGGCCAAATTCTCCCAGACCAAACATCACT
  GGTGGTGGAAGCTGCATTTTGTGTGGGAAAGAGTGAAAATTCTTCGAGATTATGCTGGCCTTATACTTTT
  CCTAGAAGAGGATCACTACTTAGCCCCAGACTTTTACCATGTCTTCAAAAAGATGTGGAAACTGAAGCAG
  CAAGAGTGCCCTGAATGTGATGTTCTCTCCCTGGGGACCTATAGTGCCAGTCGCAGTTTCTATGGCATGG
  CTGACAAGGTAGATGTGAAAACTTGGAAATCCACAGAGCACAATATGGGTCTAGCCTTGACCCGGAATGC
  CTATCAGAAGCTGATCGAGTGCACAGACACTTTCTGTACTTATGATGATTATAACTGGGACTGGACTCTT
  CAATACTTGACTGTATCTTGTCTTCCAAAATTCTGGAAAGTGCTGGTTCCTCAAATTCCTAGGATCTTTC
  ATGCTGGAGACTGTGGTATGCATCACAAGAAAACCTGTAGACCATCCACTCAGAGTGCCCAAATTGAGTC
  ACTCTTAAATAATAACAAACAATACATGTTTCCAGAAACTCTAACTATCAGTGAAAAGTTTACTGTGGTA
  GCCATTTCCCCACCTAGAAAAAATGGAGGGTGGGGAGATATTAGGGACCATGAACTCTGTAAAAGTTATA
  GAAGACTGCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC222495 representing NM_002408
  Red=Cloning site Green=Tags(s)
  
  MRFRIYKRKVLILTLVVAACGFVLWSSNGRQRKNEALAPPLLDAEPARGAGGRGGDHPSVAVGIRRVSNV
  SAASLVPAVPQPEADNLTLRYRSLVYQLNFDQTLRNVDKAGTWAPRELVLVVQVHNRPEYLRLLLDSLRK
  AQGIDNVLVIFSHDFWSTEINQLIAGVNFCPVLQVFFPFSIQLYPNEFPGSDPRDCPRDLPKNAALKLGC
  INAEYPDSFGHYREAKFSQTKHHWWWKLHFVWERVKILRDYAGLILFLEEDHYLAPDFYHVFKKMWKLKQ
  QECPECDVLSLGTYSASRSFYGMADKVDVKTWKSTEHNMGLALTRNAYQKLIECTDTFCTYDDYNWDWTL
  QYLTVSCLPKFWKVLVPQIPRIFHAGDCGMHHKKTCRPSTQSAQIESLLNNNKQYMFPETLTISEKFTVV
  AISPPRKNGGWGDIRDHELCKSYRRLQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002408
• ORF Size: 1341 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002408.1, NM_002408.2, NM_002408.3, NP_002399.1
• RefSeq Size: 2728 bp
• RefSeq ORF: 1344 bp
• Locus ID: 4247
• Cytogenetics: 14q21.3
• Domains: MGAT2
• Protein Families: Transmembrane
• Protein Pathways: Metabolic pathways, N-Glycan biosynthesis
• MW: 51.4 kDa
• Gene Summary: 'The product of this gene is a Golgi enzyme catalyzing an essential step in the conversion of oligomannose to complex N-glycans. The enzyme has the typical glycosyltransferase domains: a short N-terminal cytoplasmic domain, a hydrophobic non-cleavable signal-anchor domain, and a C-terminal catalytic domain. Mutations in this gene may lead to carbohydrate-deficient glycoprotein syndrome, type II. The coding region of this gene is intronless. Transcript variants with a spliced 5' UTR may exist, but their biological validity has not been determined. [provided by RefSeq, Jul 2008]'