MGAT2 (NM_002408) Human Untagged Clone

CAT#: SC118673

MGAT2 (untagged)-Human mannosyl (alpha-1,6-)-glycoprotein beta-1,2-N-acetylglucosaminyltransferase (MGAT2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MGAT2
• Synonyms: CDG2A; CDGS2; GLCNACTII; GNT-II; GNT2
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118673 sequence for NM_002408 edited (data generated by NextGen Sequencing)
  ATGAGGTTCCGCATCTACAAACGGAAGGTGCTAATCCTGACGCTCGTGGTGGCCGCCTGC
  GGCTTCGTCCTCTGGAGCAGCAATGGGCGACAAAGGAAGAACGAGGCCCTCGCCCCACCG
  TTGCTGGACGCCGAACCCGCGCGGGGTGCCGGCGGCCGCGGTGGGGACCACCCCTCTGTG
  GCTGTGGGCATCCGCAGGGTCTCCAACGTGTCGGCGGCTTCCCTGGTCCCGGCGGTCCCC
  CAGCCCGAGGCGGACAACCTGACGCTGCGGTACCGGTCCCTGGTGTACCAGCTGAACTTT
  GATCAGACCCTGAGGAATGTAGATAAGGCTGGCACCTGGGCCCCCCGGGAGCTGGTGCTG
  GTGGTCCAGGTGCATAACCGGCCCGAATACCTCAGACTGCTGCTGGACTCACTTCGAAAA
  GCCCAGGGAATTGACAACGTCCTCGTCATCTTTAGCCATGACTTCTGGTCGACCGAGATC
  AATCAGCTGATCGCCGGGGTGAATTTCTGTCCGGTTCTGCAGGTGTTCTTTCCTTTCAGC
  ATTCAGTTGTACCCTAACGAGTTTCCAGGTAGTGACCCTAGAGATTGTCCCAGAGACCTG
  CCGAAGAATGCCGCTTTGAAATTGGGGTGCATCAATGCTGAGTATCCCGACTCCTTCGGC
  CATTATAGAGAGGCCAAATTCTCCCAGACCAAACATCACTGGTGGTGGAAGCTGCATTTT
  GTGTGGGAAAGAGTGAAAATTCTTCGAGATTATGCTGGCCTTATACTTTTCCTAGAAGAG
  GATCACTACTTAGCCCCAGACTTTTACCATGTCTTCAAAAAGATGTGGAAACTGAAGCAG
  CAAGAGTGCCCTGAATGTGATGTTCTCTCCCTGGGGACCTATAGTGCCAGTCGCAGTTTC
  TATGGCATGGCTGACAAGGTAGATGTGAAAACTTGGAAATCCACAGAGCACAATATGGGT
  CTAGCCTTGACCCGGAATGCCTATCAGAAGCTGATCGAGTGCACAGACACTTTCTGTACT
  TATGATGATTATAACTGGGACTGGACTCTTCAATACTTGACTGTATCTTGTCTTCCAAAA
  TTCTGGAAAGTGCTGGTTCCTCAAATTCCTAGGATCTTTCATGCTGGAGACTGTGGTATG
  CATCACAAGAAAACCTGTAGACCATCCACTCAGAGTGCCCAAATTGAGTCACTCTTAAAT
  AATAACAAACAATACATGTTTCCAGAAACTCTAACTATCAGTGAAAAGTTTACTGTGGTA
  GCCATTTCCCCACCTAGAAAAAATGGAGGGTGGGGAGATATTAGGGACCATGAACTCTGT
  AAAAGTTATAGAAGACTGCAGTGA
  
  Clone variation with respect to NM_002408.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002408 unedited
  CCCCGTCGGATTTGTATACGACTCATATAGGCGGCCTGCGAATCGGCACGAGGGTCCCCG
  CCGGAGTGAGGCGAGGCCGCGTCGCTCAGTTCTGGCCGTCTAGGGCCCCTGTAAGGATGA
  GAGCGCAGAGGACGCAGGGCCGCTGGAGGCGCAGGTAACGAAGCTAGGGTGCGGTTGGGA
  CCGCGGCTGAGCTTTTTCCGGGACCCGTGGTGCTGAATGGAGAGGACGGAGACGAAGCCG
  AGCCGCGGCTCCTAGCGGCGGCGCCGATGCTCGAGCTGTAGCTGCCAGGCGAGGATGTGT
  GGGAGCGCANGCGGCGCGGGGTAAATGAGAGGTCTCGGGCCCCAGGACCCCCGGGGCCCG
  GGATGAGTTAGCGAGGGCAGCCGCGGGGGCCAGTTCCGACCGTGACAGGCCAAGGCGACG
  GCCGCCGCCCGCCCGCCCCTTCCGTGCAGAAGCAGCTGCTCCTTTCCGCGCCCGCCCGCC
  TGCGCTCCCGGCCCTGGAGACCATGAGGTTCCGCATCTACAAACGGAAGGTGCTAATCCT
  GACGCTCGTGGTGGCCGCCTGCGGCTTCGTCCTCTGGAGCAGCAATGGGCGACAAAGGAA
  GAACGAGGCCCTCGCCCCACCGTTGCTGGACGCCGAACCCGCGCGGGGTGCCGGCGGCCG
  CGGTGGGGACCACCCCTCTGTGGCTGTGGGCATCCGCAGGGTCTCCTAACGTGTCGGCGG
  CTTCCCTGGTCCCGGGGGGTCCCCCAGCCTGAGGCGGACACCCTGACGCTGCGGTACCGG
  TCCCTGGTGTACCAGCTGAACTTTGATCAGACCCTGAGGAAGTAATAAAGGCTGGCCCCT
  GGGCCCCCGGAAGTGGTGTTGGTGGTCCAGTGCATAACCGGCCCGATACCTCAGATGGTG
  CTGCACTACTTCCAA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002408
• Insert Size: 2200 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002408.3, NP_002399.1
• RefSeq Size: 2728 bp
• RefSeq ORF: 1344 bp
• Locus ID: 4247
• Cytogenetics: 14q21.3
• Domains: MGAT2
• Protein Families: Transmembrane
• Protein Pathways: Metabolic pathways, N-Glycan biosynthesis
• Gene Summary: 'The product of this gene is a Golgi enzyme catalyzing an essential step in the conversion of oligomannose to complex N-glycans. The enzyme has the typical glycosyltransferase domains: a short N-terminal cytoplasmic domain, a hydrophobic non-cleavable signal-anchor domain, and a C-terminal catalytic domain. Mutations in this gene may lead to carbohydrate-deficient glycoprotein syndrome, type II. The coding region of this gene is intronless. Transcript variants with a spliced 5' UTR may exist, but their biological validity has not been determined. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (1) represents the longer transcript. Both variants 1 and 2 encode the same protein.