Metabotropic Glutamate Receptor 7 (GRM7) (NM_181874) Human Tagged ORF Clone

CAT#: RC222540

GRM7 (Myc-DDK-tagged)-Human glutamate receptor, metabotropic 7 (GRM7), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: GRM7
• Synonyms: GLUR7; GPRC1G; MGLU7; MGLUR7; PPP1R87
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC222540 representing NM_181874
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTCCAGCTGAGGAAGCTGCTCCGCGTCCTGACTTTGATGAAGTTCCCCTGCTGCGTGCTGGAGGTGC
  TCCTGTGCGCGCTGGCGGCGGCGGCGCGCGGCCAGGAGATGTACGCCCCGCACTCAATCCGGATCGAGGG
  GGACGTCACCCTCGGGGGGCTGTTCCCCGTGCACGCCAAGGGTCCCAGCGGAGTGCCCTGCGGCGACATC
  AAGAGGGAAAACGGGATCCACAGGCTGGAAGCGATGCTCTACGCCCTGGACCAGATCAACAGTGATCCCA
  ACCTACTGCCCAACGTGACGCTGGGCGCGCGGATCCTGGACACTTGTTCCAGGGACACTTACGCGCTCGA
  ACAGTCGCTTACTTTCGTCCAGGCGCTCATCCAGAAGGACACCTCCGACGTGCGCTGCACCAACGGCGAA
  CCGCCGGTTTTCGTCAAGCCGGAGAAAGTAGTTGGAGTGATTGGGGCTTCGGGGAGTTCGGTCTCCATCA
  TGGTAGCCAACATCCTGAGGCTCTTCCAGATCCCCCAGATTAGTTATGCATCAACGGCACCCGAGCTAAG
  TGATGACCGGCGCTATGACTTCTTCTCTCGCGTGGTGCCACCCGATTCCTTCCAAGCCCAGGCCATGGTA
  GACATTGTAAAGGCCCTAGGCTGGAATTATGTGTCTACCCTCGCATCGGAAGGAAGTTATGGAGAGAAAG
  GTGTGGAGTCCTTCACGCAGATTTCCAAAGAGGCAGGTGGACTCTGCATTGCCCAGTCCGTGAGAATCCC
  CCAGGAACGCAAAGACAGGACCATTGACTTTGATAGAATTATCAAACAGCTCCTGGACACCCCCAACTCC
  AGGGCCGTCGTGATTTTTGCCAACGATGAGGATATAAAGCAGATCCTTGCAGCAGCCAAAAGAGCTGACC
  AAGTTGGCCATTTTCTTTGGGTGGGATCAGACAGCTGGGGATCCAAAATAAACCCACTGCACCAGCATGA
  AGATATCGCAGAAGGGGCCATCACCATTCAGCCCAAGCGAGCCACGGTGGAAGGGTTTGATGCCTACTTT
  ACGTCCCGTACACTTGAAAACAACAGAAGAAATGTATGGTTTGCCGAATACTGGGAGGAAAACTTCAACT
  GCAAGTTGACGATTAGTGGGTCAAAAAAAGAAGACACAGATCGCAAATGCACAGGACAGGAGAGAATTGG
  AAAAGATTCCAACTATGAGCAGGAGGGTAAAGTCCAGTTCGTGATTGACGCAGTCTATGCTATGGCTCAC
  GCCCTTCACCACATGAACAAGGATCTCTGTGCTGACTACCGGGGTGTCTGCCCAGAGATGGAGCAAGCTG
  GAGGCAAGAAGTTGCTGAAGTATATACGCAATGTTAATTTCAATGGTAGTGCTGGCACTCCAGTGATGTT
  TAACAAGAACGGGGATGCACCTGGGCGTTATGACATCTTTCAGTACCAGACCACAAACACCAGCAACCCG
  GGTTACCGTCTGATCGGGCAGTGGACAGACGAACTTCAGCTCAATATAGAAGACATGCAGTGGGGTAAAG
  GAGTCCGAGAGATACCCGCCTCAGTGTGCACACTACCATGTAAGCCAGGACAGAGAAAGAAGACACAGAA
  AGGAACTCCTTGCTGTTGGACCTGTGAGCCTTGCGATGGTTACCAGTACCAGTTTGATGAGATGACATGC
  CAGCATTGCCCCTATGACCAGAGGCCCAATGAAAATCGAACCGGATGCCAGGATATTCCCATCATCAAAC
  TGGAGTGGCACTCCCCCTGGGCTGTGATTCCTGTCTTCCTGGCAATGTTGGGGATCATTGCCACCATCTT
  TGTCATGGCCACTTTCATCCGCTACAATGACACGCCCATTGTCCGGGCATCTGGGCGGGAACTCAGCTAT
  GTTCTTTTGACGGGCATCTTTCTTTGCTACATCATCACTTTCCTGATGATTGCCAAACCAGATGTGGCAG
  TGTGTTCTTTCCGGCGAGTTTTCTTGGGCTTGGGTATGTGCATCAGTTATGCAGCCCTCTTGACGAAAAC
  AAATCGGATTTATCGCATATTTGAGCAGGGCAAGAAATCAGTAACAGCTCCCAGACTCATAAGCCCAACA
  TCACAACTGGCAATCACTTCCAGTTTAATATCAGTTCAGCTTCTAGGGGTGTTCATTTGGTTTGGTGTTG
  ATCCACCCAACATCATCATAGACTATGATGAACACAAGACAATGAACCCTGAGCAAGCCAGAGGGGTTCT
  CAAGTGTGACATTACAGATCTCCAAATCATTTGCTCCTTGGGATATAGCATTCTTCTCATGGTCACATGT
  ACTGTGTATGCCATCAAGACTCGGGGTGTACCCGAGAATTTTAACGAAGCCAAGCCCATTGGATTCACTA
  TGTACACGACATGTATAGTATGGCTTGCCTTCATTCCAATTTTTTTTGGCACCGCTCAATCAGCGGAAAA
  GCTCTACATACAAACTACCACGCTTACAATCTCCATGAACCTAAGTGCATCAGTGGCGCTGGGGATGCTA
  TACATGCCGAAAGTGTACATCATCATTTTCCACCCTGAACTCAATGTCCAGAAACGGAAGCGAAGCTTCA
  AGGCGGTAGTCACAGCAGCCACCATGTCATCGAGGCTGTCACACAAACCCAGTGACAGACCCAACGGTGA
  GGCAAAGACCGAGCTCTGTGAAAACGTAGACCCAAACAACTGTATACCACCAGTAAGAAAGAGTGTACAA
  AAGTCTGTTACTTGGTACACTATCCCACCAACAGTA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC222540 representing NM_181874
  Red=Cloning site Green=Tags(s)
  
  MVQLRKLLRVLTLMKFPCCVLEVLLCALAAAARGQEMYAPHSIRIEGDVTLGGLFPVHAKGPSGVPCGDI
  KRENGIHRLEAMLYALDQINSDPNLLPNVTLGARILDTCSRDTYALEQSLTFVQALIQKDTSDVRCTNGE
  PPVFVKPEKVVGVIGASGSSVSIMVANILRLFQIPQISYASTAPELSDDRRYDFFSRVVPPDSFQAQAMV
  DIVKALGWNYVSTLASEGSYGEKGVESFTQISKEAGGLCIAQSVRIPQERKDRTIDFDRIIKQLLDTPNS
  RAVVIFANDEDIKQILAAAKRADQVGHFLWVGSDSWGSKINPLHQHEDIAEGAITIQPKRATVEGFDAYF
  TSRTLENNRRNVWFAEYWEENFNCKLTISGSKKEDTDRKCTGQERIGKDSNYEQEGKVQFVIDAVYAMAH
  ALHHMNKDLCADYRGVCPEMEQAGGKKLLKYIRNVNFNGSAGTPVMFNKNGDAPGRYDIFQYQTTNTSNP
  GYRLIGQWTDELQLNIEDMQWGKGVREIPASVCTLPCKPGQRKKTQKGTPCCWTCEPCDGYQYQFDEMTC
  QHCPYDQRPNENRTGCQDIPIIKLEWHSPWAVIPVFLAMLGIIATIFVMATFIRYNDTPIVRASGRELSY
  VLLTGIFLCYIITFLMIAKPDVAVCSFRRVFLGLGMCISYAALLTKTNRIYRIFEQGKKSVTAPRLISPT
  SQLAITSSLISVQLLGVFIWFGVDPPNIIIDYDEHKTMNPEQARGVLKCDITDLQIICSLGYSILLMVTC
  TVYAIKTRGVPENFNEAKPIGFTMYTTCIVWLAFIPIFFGTAQSAEKLYIQTTTLTISMNLSASVALGML
  YMPKVYIIIFHPELNVQKRKRSFKAVVTAATMSSRLSHKPSDRPNGEAKTELCENVDPNNCIPPVRKSVQ
  KSVTWYTIPPTV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_181874
• ORF Size: 2766 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_181874.1, NM_181874.2, NP_870989.1
• RefSeq Size: 4239 bp
• RefSeq ORF: 2769 bp
• Locus ID: 2917
• Cytogenetics: 3p26.1
• Protein Families: Druggable Genome, GPCR, Transmembrane
• Protein Pathways: Neuroactive ligand-receptor interaction
• MW: 103.1 kDa
• Gene Summary: 'L-glutamate is the major excitatory neurotransmitter in the central nervous system, and it activates both ionotropic and metabotropic glutamate receptors. Glutamatergic neurotransmission is involved in most aspects of normal brain function and can be perturbed in many neuropathologic conditions. The metabotropic glutamate receptors are a family of G protein-coupled receptors that have been divided into three groups on the basis of sequence homology, putative signal transduction mechanisms, and pharmacologic properties. Group I includes GRM1 and GRM5, and these receptors have been shown to activate phospholipase C. Group II includes GRM2 and GRM3, while Group III includes GRM4, GRM6, GRM7 and GRM8. Group II and III receptors are linked to the inhibition of the cyclic AMP cascade but differ in their agonist selectivities. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2009]'