CLACP (COL25A1) (NM_032518) Human Tagged ORF Clone

CAT#: RC222555

COL25A1 (Myc-DDK-tagged)-Human collagen, type XXV, alpha 1 (COL25A1), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: COL25A1
• Synonyms: AMY; CFEOM5; CLAC; CLAC-P; CLACP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC222555 representing NM_032518
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGCTGAAGAAGCACGCAGGGAAAGGAGGGGGCCGGGAGCCCAGATCCGAGGACCCGACCCCTGCCG
  AACAGCATTGTGCCCGGACCATGCCCCCGTGTGCCGTCCTGGCGGCCCTCCTGTCAGTGGTGGCCGTGGT
  GTCTTGCCTGTACCTGGGTGTGAAAACCAACGACCTCCAGGCGAGGATCGCCGCTCTCGAATCCGCCAAA
  GGGGCCCCTTCCATTCATCTGCTGCCTGATACCCTGGATCACCTCAAGACTATGGTGCAAGAGAAAGTGG
  AGCGACTTCTGGCTCAGAAATCCTATGAACATATGGCTAAAATAAGAATCGCAAGAGAAGCACCTTCAGA
  ATGTAACTGCCCAGCAGGCCCTCCAGGGAAACGAGGTAAGAGAGGCCGAAGAGGAGAATCTGGTCCTCCT
  GGACAGCCTGGTCCTCAGGGCCCTCCTGGTCCAAAAGGCGATAAGGGAGAACAAGGTGATCAGGGACCTA
  GGATGGTGTTTCCTAAAATCAATCATGGGTTTCTCTCTGCTGATCAGCAGCTCATTAAACGCCGCCTGAT
  TAAGGGTGACCAAGGACAGGCAGGGCCTCCAGGACCCCCTGGCCCTCCAGGCCCAAGAGGGCCACCTGGG
  GACACAGGGAAAGATGGCCCCCGTGGAATGCCAGGAGTACCCGGTGAACCAGGAAAGCCAGGAGAACAAG
  GCTTGATGGGTCCTCTAGGGCCTCCGGGACAAAAGGGTTCTATTGGAGCACCTGGAATTCCAGGGATGAA
  TGGGCAAAAGGGTGAGCCCGGGTTGCCTGGAGCAGTAGGACAGAATGGAATACCAGGACCTAAGGGAGAA
  CCTGGAGAACAAGGTGAAAAGGGAGACGCTGGAGAGAACGGCCCCAAGGGTGACACAGGCGAAAAGGGTG
  ACCCTGGATCATCTGCTGCAGGAATTAAGGGAGAACCTGGGGAATCTGGTCGTCCAGGGCAAAAGGGTGA
  ACCAGGGCTTCCTGGGCTTCCTGGACTTCCGGGGATAAAGGGAGAACCAGGTTTCATTGGTCCTCAAGGA
  GAACCAGGCTTACCAGGTTTACCAGGAACAAAAGGTGAACGGGGGGAAGCAGGGCCTCCTGGAAGAGGTG
  AGCGAGGGGAACCTGGAGCCCCCGGACCAAAGGGGAAACAAGGTGAATCAGGAACTAGAGGCCCAAAGGG
  GTCAAAGGGGGATCGTGGAGAAAAAGGGGACTCTGGAGCTCAGGGACCAAGGGGTCCACCTGGTCAAAAA
  GGGGATCAAGGAGCCACTGAGATCATAGACTACAACGGCAACCTCCACGAAGCCTTACAGAGGATTACCA
  CCTTAACTGTCACGGGTCCCCCTGGACCTCCTGGACCTCAAGGACTACAAGGGCCAAAGGGAGAGCAGGG
  ATCTCCAGGAATCCCAGGAATGGATGGAGAGCAGGGACTCAAAGGCTCAAAGGGAGACATGGGGGACCCA
  GGTATGACAGGTGAAAAAGGAGGAATTGGACTTCCTGGATTACCGGGAGCCAATGGAATGAAAGGAGAAA
  AAGGAGATTCTGGAATGCCGGGTCCACAGGGTCCTTCTATCATAGGCCCACCAGGCCCACCAGGTCCCCA
  TGGCCCACCTGGCCCCATGGGACCTCATGGACTTCCTGGACCAAAGGGTACAGATGGTCCTATGGGACCC
  CATGGCCCTGCAGGTCCCAAAGGAGAAAGAGGTGAAAAAGGAGCTATGGGAGAGCCTGGACCAAGAGGGC
  CCTATGGGCTGCCTGGGAAAGATGGAGAGCCTGGTCTTGATGGCTTCCCTGGTCCACGGGGTGAGAAGGG
  TGATCTAGGAGAAAAGGGAGAAAAGGTGACCTCTCCATCCCAGCATGTACCTTGTTTGATTCTTCTCCTG
  CTGTCTGCTTTGCTGTTCAGCTTGTGTGATTCCATT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC222555 representing NM_032518
  Red=Cloning site Green=Tags(s)
  
  MLLKKHAGKGGGREPRSEDPTPAEQHCARTMPPCAVLAALLSVVAVVSCLYLGVKTNDLQARIAALESAK
  GAPSIHLLPDTLDHLKTMVQEKVERLLAQKSYEHMAKIRIAREAPSECNCPAGPPGKRGKRGRRGESGPP
  GQPGPQGPPGPKGDKGEQGDQGPRMVFPKINHGFLSADQQLIKRRLIKGDQGQAGPPGPPGPPGPRGPPG
  DTGKDGPRGMPGVPGEPGKPGEQGLMGPLGPPGQKGSIGAPGIPGMNGQKGEPGLPGAVGQNGIPGPKGE
  PGEQGEKGDAGENGPKGDTGEKGDPGSSAAGIKGEPGESGRPGQKGEPGLPGLPGLPGIKGEPGFIGPQG
  EPGLPGLPGTKGERGEAGPPGRGERGEPGAPGPKGKQGESGTRGPKGSKGDRGEKGDSGAQGPRGPPGQK
  GDQGATEIIDYNGNLHEALQRITTLTVTGPPGPPGPQGLQGPKGEQGSPGIPGMDGEQGLKGSKGDMGDP
  GMTGEKGGIGLPGLPGANGMKGEKGDSGMPGPQGPSIIGPPGPPGPHGPPGPMGPHGLPGPKGTDGPMGP
  HGPAGPKGERGEKGAMGEPGPRGPYGLPGKDGEPGLDGFPGPRGEKGDLGEKGEKVTSPSQHVPCLILLL
  LSALLFSLCDSI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_032518
• ORF Size: 1926 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_032518.1, NM_032518.2, NP_115907.2
• RefSeq Size: 2681
• RefSeq ORF: 1929
• Locus ID: 84570
• Domains: Collagen
• Protein Families: Transmembrane
• MW: 63.5 kDa
• Gene Summary: This gene encodes a brain-specific membrane associated collagen. A product of proteolytic processing of the encoded protein, CLAC (collagenous Alzheimer amyloid plaque component), binds to amyloid beta-peptides found in Alzheimer amyloid plaques but CLAC inhibits rather than facilitates amyloid fibril elongation (PMID: 16300410). A study of over-expression of this collagen in mice, however, found changes in pathology and behavior suggesting that the encoded protein may promote amyloid plaque formation (PMID: 19548013). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]