ARVCF (NM_001670) Human Tagged ORF Clone

CAT#: RC222598

ARVCF (Myc-DDK-tagged)-Human armadillo repeat gene deleted in velocardiofacial syndrome (ARVCF)

Product Images

Western blot validation of overexpression lysate (Cat# LY419792) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC222598 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ARVCF
• Synonyms: FLJ35345
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC222598 representing NM_001670
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGACTGCAATGTGCACTCGGCCGCCAGCATCCTGGCCTCGGTGAAGGAGCAGGAGGCCCGCTTCG
  AGAGGCTGACACGGGCACTGGAGCAGGAGCGGCGCCATGTTGCCCTACAGCTGGAGCGTGCCCAGCAGCC
  TGGCATGGTCAGTGGTGGCATGGGCAGTGGGCAGCCCCTGCCAATGGCCTGGCAACAGCTGGTCCTCCAG
  GAGCAGAGCCCAGGCAGCCAGGCCTCACTGGCCACGATGCCGGAGGCACCTGATGTGCTGGAGGAGACCG
  TGACGGTGGAGGAGGACCCCGGCACACCCACTTCCCATGTGTCTATTGTCACATCCGAAGATGGCACAAC
  CCGGCGCACCGAGACCAAGGTCACCAAGACTGTCAAGACGGTGACCACTCGGACAGTACGCCAGGTGCCC
  GTGGGCCCAGATGGACTCCCCCTGCTGGATGGCGGCCCCCCACTAGGCCCTTTTGCAGATGGTGCCCTGG
  ACCGGCATTTCCTGCTGCGTGGTGGTGGCCCAGTGGCCACACTCTCTCGAGCCTACCTCAGCAGTGGGGG
  TGGCTTTCCCGAAGGCCCCGAGCCCCGGGACAGCCCCAGCTATGGCAGCCTGTCCCGAGGGCTGGGCATG
  CGGCCCCCACGTGCTGGCCCCCTTGGCCCAGGCCCTGGTGATGGCTGCTTCACACTGCCTGGCCACCGGG
  AAGCCTTCCCGGTGGGTCCTGAGCCTGGGCCACCAGGTGGCCGCTCCCTGCCCGAGCGCTTCCAGGCAGA
  GCCGTATGGCTTGGAGGATGACACGCGCAGCCTGGCCGCTGATGACGAGGGTGGCCCTGAGCTGGAGCCT
  GACTATGGCACGGCCACAAGGAGGAGGCCTGAGTGTGGGCGGGGCCTTCATACCAGGGCCTACGAGGACA
  CAGCAGATGATGGCGGCGAGCTGGCGGACGAGCGGCCTGCGTTCCCAATGGTGACGGCGCCCCTGGCCCA
  GCCTGAACGGGGCAGCATGGGCAGCCTGGACCGGCTGGTGCGGCGCTCGCCCTCAGTGGATAGCGCCCGC
  AAGGAGCCGCGCTGGCGGGACCCTGAGCTGCCTGAGGTGCTGGCCATGCTGCGGCACCCCGTGGACCCCG
  TGAAGGCCAATGCGGCCGCCTACCTGCAGCATCTGTGCTTTGAGAACGAGGGTGTCAAGCGGCGTGTACG
  GCAGTTGCGGGGGCTGCCGCTGCTTGTGGCACTGCTGGACCACCCGCGGGCTGAGGTGCGGCGCCGGGCC
  TGTGGGGCACTGCGCAACCTCTCCTATGGCCGCGACACTGACAACAAGGCCGCCATCCGGGACTGCGGTG
  GTGTGCCTGCCCTGGTGCGCCTGCTGAGGGCTGCCCGGGACAACGAGGTCCGTGAGCTTGTCACTGGCAC
  CCTGTGGAACCTGTCATCCTATGAGCCCCTGAAGATGGTCATCATTGACCATGGCCTGCAGACGCTGACC
  CACGAGGTGATCGTGCCCCACTCAGGATGGGAGCGTGAGCCCAACGAGGACTCCAAGCCACGGGACGCCG
  AGTGGACAACTGTCTTCAAGAACACGTCGGGCTGCCTGAGGAATGTGAGCTCCGATGGTGCTGAGGCCCG
  GCGGCGACTCCGGGAGTGTGAAGGGCTGGTGGACGCGCTCCTGCATGCCCTGCAGTCGGCTGTGGGCCGG
  AAGGACACTGACAACAAGTCGGTGGAGAACTGCGTGTGCATCATGCGGAACCTGTCCTACCACGTGCACA
  AGGAGGTGCCCGGGGCCGACAGGTACCAGGAGGCCGAGCCCGGGCCCCTGGGCAGTGCTGTAGGCTCCCA
  GCGCCGGAGGCGGGATGATGCCAGCTGCTTTGGAGGCAAGAAGGCCAAAGAGGAGTGGTTCCACCAAGGA
  AAGAAGGATGGTGAGATGGACCGGAACTTTGACACGCTAGACCTGCCCAAGCGAACTGAGGCCGCCAAAG
  GCTTTGAGCTGCTGTACCAGCCCGAGGTGGTACGTCTCTACCTCTCCCTCCTCACGGAGAGCCGGAACTT
  CAACACCCTGGAGGCTGCCGCCGGCGCTCTGCAGAACCTCAGTGCCGGCAACTGGATGTGGGCCACGTAC
  ATCCGCGCCACAGTGCGCAAAGAGCGCGGGCTGCCGGTGCTTGTGGAACTGCTGCAGTCTGAGACCGACA
  AGGTGGTGCGCGCCGTCGCCATCGCTCTGCGCAACCTCTCGCTGGACCGGCGCAACAAAGACCTCATCGG
  GAGCTACGCCATGGCTGAGCTTGTGCGGAATGTGCGCAATGCACAGGCTCCGCCGCGACCGGGGGCCTGC
  CTGGAGGAAGACACCGTGGTGGCGGTGCTCAACACCATCCACGAAATCGTGTCCGACAGCCTGGATAACG
  CGCGCTCGCTCCTGCAGGCACGCGGGGTGCCAGCGTTGGTGGCTCTCGTGGCCTCCAGCCAATCGGTACG
  CGAAGCGAAGGCGGCGTCACACGTGCTGCAGACAGTGTGGAGCTACAAGGAGCTGCGTGGTACCTTGCAG
  AAAGATGGTTGGACCAAGGCGCGCTTCCAGTCAGCTGCTGCTACTGCCAAGGGGCCTAAGGGAGCACTGA
  GTCCTGGGGGCTTCGATGACAGCACGCTGCCACTGGTGGACAAGAGCCTTGAGGGCGAGAAAACTGGCAG
  CCGGGATGTGATCCCCATGGATGCGCTGGGCCCAGACGGATACTCCACGGTGGACCGGAGGGAGCGGAGG
  CCACGGGGCGCCAGCTCTGCAGGAGAGGCCTCTGAGAAGGAACCCTTGAAACTCGACCCCAGCAGGAAGG
  CCCCTCCCCCCGGGCCCAGCAGGCCCGCGGTCAGGCTGGTGGACGCCGTAGGGGACGCTAAGCCTCAGCC
  CGTTGATTCCTGGGTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC222598 representing NM_001670
  Red=Cloning site Green=Tags(s)
  
  MEDCNVHSAASILASVKEQEARFERLTRALEQERRHVALQLERAQQPGMVSGGMGSGQPLPMAWQQLVLQ
  EQSPGSQASLATMPEAPDVLEETVTVEEDPGTPTSHVSIVTSEDGTTRRTETKVTKTVKTVTTRTVRQVP
  VGPDGLPLLDGGPPLGPFADGALDRHFLLRGGGPVATLSRAYLSSGGGFPEGPEPRDSPSYGSLSRGLGM
  RPPRAGPLGPGPGDGCFTLPGHREAFPVGPEPGPPGGRSLPERFQAEPYGLEDDTRSLAADDEGGPELEP
  DYGTATRRRPECGRGLHTRAYEDTADDGGELADERPAFPMVTAPLAQPERGSMGSLDRLVRRSPSVDSAR
  KEPRWRDPELPEVLAMLRHPVDPVKANAAAYLQHLCFENEGVKRRVRQLRGLPLLVALLDHPRAEVRRRA
  CGALRNLSYGRDTDNKAAIRDCGGVPALVRLLRAARDNEVRELVTGTLWNLSSYEPLKMVIIDHGLQTLT
  HEVIVPHSGWEREPNEDSKPRDAEWTTVFKNTSGCLRNVSSDGAEARRRLRECEGLVDALLHALQSAVGR
  KDTDNKSVENCVCIMRNLSYHVHKEVPGADRYQEAEPGPLGSAVGSQRRRRDDASCFGGKKAKEEWFHQG
  KKDGEMDRNFDTLDLPKRTEAAKGFELLYQPEVVRLYLSLLTESRNFNTLEAAAGALQNLSAGNWMWATY
  IRATVRKERGLPVLVELLQSETDKVVRAVAIALRNLSLDRRNKDLIGSYAMAELVRNVRNAQAPPRPGAC
  LEEDTVVAVLNTIHEIVSDSLDNARSLLQARGVPALVALVASSQSVREAKAASHVLQTVWSYKELRGTLQ
  KDGWTKARFQSAAATAKGPKGALSPGGFDDSTLPLVDKSLEGEKTGSRDVIPMDALGPDGYSTVDRRERR
  PRGASSAGEASEKEPLKLDPSRKAPPPGPSRPAVRLVDAVGDAKPQPVDSWV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001670
• ORF Size: 2886 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001670.1, NM_001670.2, NP_001661.1
• RefSeq Size: 4056 bp
• RefSeq ORF: 2889 bp
• Locus ID: 421
• Cytogenetics: 22q11.21
• MW: 104.6 kDa
• Gene Summary: 'Armadillo Repeat gene deleted in Velo-Cardio-Facial syndrome (ARVCF) is a member of the catenin family. This family plays an important role in the formation of adherens junction complexes, which are thought to facilitate communication between the inside and outside environments of a cell. The ARVCF gene was isolated in the search for the genetic defect responsible for the autosomal dominant Velo-Cardio-Facial syndrome (VCFS), a relatively common human disorder with phenotypic features including cleft palate, conotruncal heart defects and facial dysmorphology. The ARVCF gene encodes a protein containing two motifs, a coiled coil domain in the N-terminus and a 10 armadillo repeat sequence in the midregion. Since these sequences can facilitate protein-protein interactions ARVCF is thought to function in a protein complex. In addition, ARVCF contains a predicted nuclear-targeting sequence suggesting that it may have a function as a nuclear protein. [provided by RefSeq, Jun 2010]'