ARVCF (NM_001670) Human Untagged Clone

CAT#: SC315001

ARVCF (untagged)-Human armadillo repeat gene deleted in velocardiofacial syndrome (ARVCF)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ARVCF
• Synonyms: FLJ35345
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_001670 edited
  ATGGAGGACTGCAATGTGCACTCGGCCGCCAGCATCCTGGCCTCGGTGAAGGAGCAGGAG
  GCCCGCTTCGAGAGGCTGACACGGGCACTGGAGCAGGAGCGGCGCCATGTTGCCCTACAG
  CTGGAGCGTGCCCAGCAGCCTGGCATGGTCAGTGGTGGCATGGGCAGTGGGCAGCCCCTG
  CCAATGGCCTGGCAACAGCTGGTCCTCCAGGAGCAGAGCCCAGGCAGCCAGGCCTCACTG
  GCCACGATGCCGGAGGCACCTGATGTGCTGGAGGAGACCGTGACGGTGGAGGAGGACCCC
  GGCACACCCACTTCCCATGTGTCTATTGTAACATCCGAAGATGGCACAACCCGGCGCACC
  GAGACCAAGGTCACCAAGACTGTCAAGACGGTGACCACTCGGACAGTACGCCAGGTGCCC
  GTGGGCCCAGATGGACTCCCCCTGCTGGATGGCGGCCCCCCACTAGGCCCTTTTGCAGAT
  GGTGCCCTGGACCGGCATTTCCTGCTGCGTGGTGGTGGCCCAGCGGCCACACTCTCTCGA
  GCCTACCTCAGCAGTGGGGGTGGCTTTCCCGAAGGCCCCGAGCCCCGGGACAGCCCCAGC
  TATGGCAGCCTGTCCCGAGGGCTGGGCATGCGGCCCCCACGTGCTGGCCCCCTTGGCCCA
  GGCCCTGGTGATGGCTGCTTCACACTGCCTGGCCACCGGGAAGCCTTCCCGGTGGGTCCT
  GAGCCTGGGCCACCAGGTGGCCGCTCCCTGCCCGAGCGCTTCCAGGCAGAGCCGTATGGC
  TTGGAGGATGACACGCGCAGCCTGGCCGCTGATGACGAGGGTGGCCCTGAGCTGGAGCCT
  GACTATGGCACGGCCACAAGGAGGAGGCCTGAGTGTGGGCGGGGCCTTCATACCAGGGCC
  TACGAGGACACAGCAGATGATGGCGGCGAGCTGGCGGACGAGCGGCCTGCGTTCCCAATG
  GTGACGGCGCCCCTGGCCCAGCCTGAACGGGGCAGCATGGGCAGCCTGGACCGGCTGGTG
  CGGCGCTCGCCCTCAGTGGATAGCGCCCGCAAGGAGCCGCGCTGGCGGGACCCTGAGCTG
  CCTGAGGTGCTGGCCATGCTGCGGCACCCCGTGGACCCCGTGAAGGCCAATGCGGCCGCC
  TACCTGCAGCATCTGTGCTTTGAGAACGAGGGTGTCAAGCGGCGTGTACGGCAGTTGCGG
  GGGCTGCCGCTGCTTGTGGCACTGCTGGACCACCCGCGGGCTGAGGTGCGGCGCCGGGCC
  TGTGGGGCACTGCGCAACCTCTCCTATGGCCGCGACACTGACAACAAGGCCGCCATCCGG
  GACTGCGGTGGTGTGCCTGCCCTGGTGCGCCTGCTGAGGGCTGCCCGGGACAACGAGGTC
  CGTGAGCTTGTCACTGGCACCCTGTGGAACCTGTCATCCTATGAGCCCCTGAAGATGGTC
  ATCATTGACCATGGCCTGCAGACGCTGACCCACGAGGTGATCGTGCCCCACTCAGGATGG
  GAGCGTGAGCCCAACGAGGACTCCAAGCCACGGGACGCCGAGTGGACAACTGTCTTCAAG
  AACACGTCGGGCTGCCTGAGGAATGTGAGCTCCGATGGTGCTGAGGCCCGGCGGCGACTC
  CGGGAGTGTGAAGGGCTGGTGGACGCGCTCCTGCATGCCCTGCAGTCGGCTGTGGGCCGG
  AAGGACACTGACAACAAGTCGGTGGAGAACTGCGTGTGCATCATGCGGAACCTGTCCTAC
  CACGTGCACAAGGAGGTGCCCGGGGCCGACAGGTACCAGGAGGCCGAGCCCGGGCCCCTG
  GGCAGTGCTGTAGGCTCCCAGCGCCGGAGGCGGGATGATGCCAGCTGCTTTGGAGGCAAG
  AAGGCCAAAGAGGGAAAGAAGGATGGTGAGATGGACCGGAACTTTGACACGCTAGACCTG
  CCCAAGCGAACTGAGGCCGCCAAAGGCTTTGAGCTGCTGTACCAGCCCGAGGTGGTACGT
  CTCTACCTCTCCCTCCTCACGGAGAGCCGGAACTTCAACACCCTGGAGGCTGCCGCCGGC
  GCTCTGCAGAACCTCAGTGCCGGCAACTGGATGTGGGCCACGTACATCCGCGCCACAGTG
  CGCAAAGAGCGCGGGCTGCCGGTGCTTGTGGAACTGCTGCAGTCTGAGACCGACAAGGTG
  GTGCGCGCCGTCGCCATCGCTCTGCGCAACCTCTCGCTGGACCGGCGCAACAAAGACCTC
  ATCGGGAGCTACGCCATGGCTGAGCTTGTGCGGAATGTGCGCAATGCACAGGCTCCGCCG
  CGACCGGGGGCCTGCCTGGAGGAAGACACCGTGGTGGCGGTGCTCAACACCATCCACGAA
  ATCGTGTCCGACAGCCTGGATAACGCGCGCTCGCTCCTGCAGGCACGCGGGGTGCCAGCG
  TTGGTGGCTCTCGTGGCCTCCAGCCAATCGGTACGCGAAGCGAAGGCGGCGTCACACGTG
  CTGCAGACAGTGTGGAGCTACAAGGAGCTGCGTGGTACCTTGCAGAAAGATGGTTGGACC
  AAGGCGCGCTTCCAGTCAGCTGCTGCTACTGCCAAGGGGCCTAAGGGAGCACTGAGTCCT
  GGGGGCTTCGATGACAGCACGCTGCCACTGGTGGACAAGAGCCTTGAGGGCGAGAAAACT
  GGCAGCCGGGATGTGATCCCCATGGATGCGCTGGGCCCAGACGGATACTCCACGGTGGAC
  CGGAGGGAGCGGAGGCCACGGGGCGCCAGCTCTGCAGGAGAGGCCTCTGAGAAGGAACCC
  TTGAAACTCGACCCCAGCAGGAAGGCCCCTCCCCCCGGGCCCAGCAGGCCCGCGGTCAGG
  CTGGTGGACGCCGTAGGGGACGCTAAGCCTCAGCCCGTTGATTCCTGGGTCTAG
  
• Restriction Sites: Please inquire
• ACCN: NM_001670
• Insert Size: 3800 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001670.1, NP_001661.1
• RefSeq Size: 4031 bp
• RefSeq ORF: 2889 bp
• Locus ID: 421
• Cytogenetics: 22q11.21
• Gene Summary: 'Armadillo Repeat gene deleted in Velo-Cardio-Facial syndrome (ARVCF) is a member of the catenin family. This family plays an important role in the formation of adherens junction complexes, which are thought to facilitate communication between the inside and outside environments of a cell. The ARVCF gene was isolated in the search for the genetic defect responsible for the autosomal dominant Velo-Cardio-Facial syndrome (VCFS), a relatively common human disorder with phenotypic features including cleft palate, conotruncal heart defects and facial dysmorphology. The ARVCF gene encodes a protein containing two motifs, a coiled coil domain in the N-terminus and a 10 armadillo repeat sequence in the midregion. Since these sequences can facilitate protein-protein interactions ARVCF is thought to function in a protein complex. In addition, ARVCF contains a predicted nuclear-targeting sequence suggesting that it may have a function as a nuclear protein. [provided by RefSeq, Jun 2010]'