CBX2 (NM_005189) Human Tagged ORF Clone

CAT#: RC222700

CBX2 (Myc-DDK-tagged)-Human chromobox homolog 2 (CBX2), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY417456) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC222700 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CBX2
• Synonyms: CDCA6; M33; SRXY5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC222700 representing NM_005189
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGAGCTGAGCAGCGTGGGCGAGCAGGTCTTCGCCGCCGAGTGCATCCTGAGCAAGCGGCTCCGCA
  AGGGCAAGCTGGAGTACCTGGTCAAGTGGCGCGGCTGGTCCTCCAAACATAACAGCTGGGAGCCGGAGGA
  GAACATCCTGGACCCGAGGCTGCTCCTGGCCTTCCAGAAGAAGGAACATGAGAAGGAGGTGCAGAACCGG
  AAGAGAGGCAAGAGGCCGAGAGGCCGGCCAAGGAAGCTCACTGCCATGTCCTCCTGCAGCCGGCGCTCCA
  AGCTCAAGGAACCCGATGCTCCCTCCAAATCCAAGTCCAGCAGTTCCTCCTCTTCCTCCACGTCATCCTC
  CTCTTCCTCAGATGAAGAGGATGACAGTGACTTAGATGCTAAGAGGGGTCCCCGGGGCCGCGAGACCCAC
  CCAGTGCCGCAGAAGAAGGCCCAGATCCTGGTGGCCAAACCCGAGCTGAAGGATCCCATCCGGAAGAAGC
  GGGGACGAAAGCCCCTGCCCCCAGAGCAAAAGGCAACCCGAAGACCCGTGAGCCTGGCCAAGGTGCTGAA
  GACCGCCCGGAAGGATCTGGGGGCCCCGGCCAGCAAGCTGCCCCCTCCACTCAGCGCCCCCGTTGCAGGC
  CTGGCAGCTCTGAAGGCCCACGCCAAGGAGGCCTGTGGCGGCCCCAGTGCCATGGCCACCCCAGAGAACC
  TGGCCAGCCTAATGAAGGGCATGGCCAGTAGCCCCGGCCGGGGTGGCATCAGCTGGCAGAGCTCCATCGT
  GCACTACATGAACCGGATGACCCAGAGCCAGGCCCAGGCTGCCAGCAGGTTGGCGCTGAAGGCCCAGGCC
  ACCAACAAGTGCGGCCTCGGGCTGGACCTGAAGGTGAGGACGCAGAAAGGGGAGCTGGGAATGAGCCCTC
  CAGGAAGCAAAATCCCGAAGGCCCCCAGCGGTGGGGCTGTGGAGCAGAAAGTGGGGAACACAGGGGGCCC
  CCCGCACACCCATGGTGCCAGCAGGGTGCCTGCTGGGTGCCCAGGCCCCCAGCCAGCACCCACCCAGGAG
  CTGAGCCTCCAGGTCTTGGACTTGCAGAGTGTCAAGAATGGCATGCCCGGGGTGGGTCTCCTTGCCCGCC
  ACGCCACCGCCACCAAGGGTGTCCCGGCCACCAACCCAGCCCCTGGGAAGGGCACTGGGAGTGGCCTCAT
  TGGGGCCAGCGGGGCCACCATGCCCACCGACACAAGCAAAAGTGAGAAGCTGGCTTCCAGAGCAGTGGCG
  CCACCCACCCCTGCCAGCAAGAGGGACTGTGTCAAGGGCAGTGCTACCCCCAGTGGGCAGGAGAGCCGCA
  CAGCCCCCGGAGAAGCCCGCAAGGCGGCCACACTGCCAGAGATGAGCGCAGGTGAGGAGAGTAGCAGCTC
  GGACTCCGACCCCGACTCCGCCTCGCCGCCCAGCACTGGACAGAACCCGTCAGTGTCCGTTCAGACCAGC
  CAGGACTGGAAGCCCACCCGCAGCCTCATCGAGCACGTATTTGTCACCGACGTCACTGCCAACCTCATCA
  CCGTCACAGTGAAGGAGTCTCCCACCAGCGTGGGCTTCTTCAACCTGAGGCATTAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC222700 representing NM_005189
  Red=Cloning site Green=Tags(s)
  
  MEELSSVGEQVFAAECILSKRLRKGKLEYLVKWRGWSSKHNSWEPEENILDPRLLLAFQKKEHEKEVQNR
  KRGKRPRGRPRKLTAMSSCSRRSKLKEPDAPSKSKSSSSSSSSTSSSSSSDEEDDSDLDAKRGPRGRETH
  PVPQKKAQILVAKPELKDPIRKKRGRKPLPPEQKATRRPVSLAKVLKTARKDLGAPASKLPPPLSAPVAG
  LAALKAHAKEACGGPSAMATPENLASLMKGMASSPGRGGISWQSSIVHYMNRMTQSQAQAASRLALKAQA
  TNKCGLGLDLKVRTQKGELGMSPPGSKIPKAPSGGAVEQKVGNTGGPPHTHGASRVPAGCPGPQPAPTQE
  LSLQVLDLQSVKNGMPGVGLLARHATATKGVPATNPAPGKGTGSGLIGASGATMPTDTSKSEKLASRAVA
  PPTPASKRDCVKGSATPSGQESRTAPGEARKAATLPEMSAGEESSSSDSDPDSASPPSTGQNPSVSVQTS
  QDWKPTRSLIEHVFVTDVTANLITVTVKESPTSVGFFNLRHY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005189
• ORF Size: 1596 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_005189.1, NM_005189.2, NP_005180.1
• RefSeq Size: 4192
• RefSeq ORF: 1599
• Locus ID: 84733
• Protein Families: Transcription Factors
• MW: 55.9 kDa
• Gene Summary: This gene encodes a component of the polycomb multiprotein complex, which is required to maintain the transcriptionally repressive state of many genes throughout development via chromatin remodeling and modification of histones. Disruption of this gene in mice results in male-to-female gonadal sex reversal. Mutations in this gene are also associated with gonadal dysgenesis in humans. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Mar 2010]