SIM1 (NM_005068) Human Tagged ORF Clone

CAT#: RC222805

SIM1 (Myc-DDK-tagged)-Human single-minded homolog 1 (Drosophila) (SIM1)

Product Images

Western blot validation of overexpression lysate (Cat# LY417552) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC222805 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SIM1
• Synonyms: bHLHe14
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC222805 representing NM_005068
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAAGAAAAGTCCAAAAATGCTGCGCGGACTAGGAGGGAGAAGGAAAACAGTGAATTTTATGAACTGG
  CTAAATTACTGCCTTTGCCCTCGGCTATCACCTCGCAGCTGGACAAAGCATCCATAATCAGACTCACGAC
  CAGCTATCTCAAAATGAGAGTGGTGTTCCCAGAAGGGCTCGGCGAGGCGTGGGGCCACTCAAGTCGGACC
  AGCCCCCTGGACAACGTTGGCCGAGAACTGGGCTCCCATCTGCTCCAGACCCTGGATGGCTTCATCTTCG
  TGGTAGCCCCAGATGGGAAGATCATGTACATCTCAGAGACAGCCTCAGTCCACTTGGGTCTTTCTCAGGT
  AGAGCTGACCGGAAACAGCATTTATGAATACATTCACCCGGCAGACCACGACGAGATGACGGCGGTGCTC
  ACCGCCCATCAACCCTACCACTCTCACTTCGTGCAGGAGTATGAGATCGAGCGCTCCTTCTTCCTGAGGA
  TGAAGTGCGTCTTGGCCAAGCGTAACGCCGGCCTCACCTGTGGCGGCTACAAGGTCATCCACTGCAGCGG
  CTACTTGAAGATCCGCCAGTACAGCCTGGACATGTCCCCCTTCGACGGCTGCTACCAAAACGTGGGCCTG
  GTGGCCGTGGGCCACTCGCTGCCTCCCAGCGCCGTCACGGAGATCAAGCTACACAGCAATATGTTTATGT
  TCCGCGCCAGCCTGGACATGAAGCTCATCTTTCTGGACTCCAGGGTGGCGGAGCTGACGGGGTACGAACC
  TCAGGACCTGATTGAGAAGACTCTGTACCACCATGTGCACGGCTGCGACACCTTCCACCTGCGCTGCGCG
  CACCATTTGCTGCTGGTGAAGGGACAGGTGACCACCAAGTACTACAGGTTCCTGGCGAAACACGGCGGCT
  GGGTATGGGTGCAGAGCTACGCGACCATCGTGCACAACAGTCGCTCCTCCAGGCCACACTGTATCGTCAG
  CGTCAACTATGTCCTCACAGACACAGAATACAAAGGGCTGCAGCTCTCCCTGGATCAGATCTCAGCCTCC
  AAACCAGCCTTCTCCTATACCAGCAGCTCCACCCCCACCATGACTGACAACAGAAAGGGGGCCAAATCCC
  GGCTCTCCAGCTCAAAGTCAAAATCCAGGACTTCCCCATACCCTCAGTATTCGGGATTTCACACAGAAAG
  ATCGGAATCTGATCATGACAGCCAGTGGGGCGGAAGTCCCTTGACCGACACGGCCTCTCCGCAGCTTCTG
  GACCCCGCCGATAGGCCTGGCTCCCAGCACGACGCATCGTGCGCCTACAGACAGTTTTCGGACCGCAGCT
  CTCTCTGCTATGGCTTTGCGCTTGACCACTCGAGGCTGGTGGAAGAGAGGCATTTCCATACCCAGGCCTG
  TGAAGGAGGCCGATGTGAGGCAGGCAGGTACTTCCTGGGAACGCCGCAGGCCGGGAGGGAGCCCTGGTGG
  GGCTCTCGCGCAGCCTTGCCCCTGACAAAGGCCTCCCCAGAAAGCAGAGAAGCCTATGAAAACAGCATGC
  CTCACATCGCTTCAGTCCACAGGATCCATGGGCGAGGTCATTGGGATGAAGATAGTGTGGTCAGTTCTCC
  AGACCCTGGGTCGGCCAGTGAATCAGGTGACCGATATCGTACTGAGCAGTATCAAAGTAGCCCACATGAA
  CCCAGCAAAATTGAAACTCTTATAAGAGCCACTCAGCAAATGATTAAAGAAGAAGAGAACAGATTACAGC
  TAAGGAAAGCCCCCTCAGACCAACTGGCTTCCATTAATGGGGCTGGGAAAAAACACTCCCTGTGTTTTGC
  AAACTACCAACAGCCCCCACCAACAGGTGAAGTCTGCCATGGCTCTGCTCTTGCCAACACTTCACCATGT
  GACCATATCCAGCAGAGAGAGGGAAAAATGTTGAGCCCCCATGAAAATGACTATGACAACAGTCCCACCG
  CACTATCTCGGATAAGTAGTCCCAATTCGGATCGCATTTCAAAATCCAGTTTGATCCTAGCTAAAGACTA
  TCTGCATTCGGATATATCTCCTCATCAGACAGCAGGAGACCACCCTACTGTCTCTCCAAACTGCTTTGGC
  TCTCACCGGCAGTATTTTGACAAGCATGCTTACACATTAACTGGATATGCCCTGGAGCACTTATATGACA
  GCGAAACCATTAGAAACTATTCCTTGGGCTGTAATGGCTCACACTTTGATGTAACTTCCCATCTGAGGAT
  GCAACCAGACCCAGCACAAGGACACAAGGGAACATCTGTTATAATAACCAACGGAAGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC222805 representing NM_005068
  Red=Cloning site Green=Tags(s)
  
  MKEKSKNAARTRREKENSEFYELAKLLPLPSAITSQLDKASIIRLTTSYLKMRVVFPEGLGEAWGHSSRT
  SPLDNVGRELGSHLLQTLDGFIFVVAPDGKIMYISETASVHLGLSQVELTGNSIYEYIHPADHDEMTAVL
  TAHQPYHSHFVQEYEIERSFFLRMKCVLAKRNAGLTCGGYKVIHCSGYLKIRQYSLDMSPFDGCYQNVGL
  VAVGHSLPPSAVTEIKLHSNMFMFRASLDMKLIFLDSRVAELTGYEPQDLIEKTLYHHVHGCDTFHLRCA
  HHLLLVKGQVTTKYYRFLAKHGGWVWVQSYATIVHNSRSSRPHCIVSVNYVLTDTEYKGLQLSLDQISAS
  KPAFSYTSSSTPTMTDNRKGAKSRLSSSKSKSRTSPYPQYSGFHTERSESDHDSQWGGSPLTDTASPQLL
  DPADRPGSQHDASCAYRQFSDRSSLCYGFALDHSRLVEERHFHTQACEGGRCEAGRYFLGTPQAGREPWW
  GSRAALPLTKASPESREAYENSMPHIASVHRIHGRGHWDEDSVVSSPDPGSASESGDRYRTEQYQSSPHE
  PSKIETLIRATQQMIKEEENRLQLRKAPSDQLASINGAGKKHSLCFANYQQPPPTGEVCHGSALANTSPC
  DHIQQREGKMLSPHENDYDNSPTALSRISSPNSDRISKSSLILAKDYLHSDISPHQTAGDHPTVSPNCFG
  SHRQYFDKHAYTLTGYALEHLYDSETIRNYSLGCNGSHFDVTSHLRMQPDPAQGHKGTSVIITNGS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005068
• ORF Size: 2298 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005068.1, NM_005068.2, NP_005059.2
• RefSeq Size: 3995 bp
• RefSeq ORF: 2301 bp
• Locus ID: 6492
• Cytogenetics: 6q16.3
• Protein Families: Druggable Genome, Transcription Factors
• MW: 85.3 kDa
• Gene Summary: 'SIM1 and SIM2 genes are Drosophila single-minded (sim) gene homologs. SIM1 transcript was detected only in fetal kidney out of various adult and fetal tissues tested. Since the sim gene plays an important role in Drosophila development and has peak levels of expression during the period of neurogenesis,it was proposed that the human SIM gene is a candidate for involvement in certain dysmorphic features (particularly the facial and skull characteristics), abnormalities of brain development, and/or cognitive disability of Down syndrome. [provided by RefSeq, Jul 2008]'