Collagen V (COL5A3) (NM_015719) Human Tagged ORF Clone

CAT#: RC222823

COL5A3 (Myc-DDK-tagged)-Human collagen, type V, alpha 3 (COL5A3)

Product Images

Western blot validation of overexpression lysate (Cat# LY414351) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC222823 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: COL5A3
• Synonyms: collagen, type V, alpha 3; OTTHUMP00000195993; pro-(alpha)3(V) collagen
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC222823 representing NM_015719
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGAACCGCCGGGACCTGGGCCAGCCGCGGGCCGGTCTCTGCCTGCTCCTGGCCGCGCTGCAGCTTC
  TGCCGGGGACGCAGGCCGATCCTGTGGATGTCCTGAAGGCCCTGGGTGTGCAGGGAGGCCAGGCTGGGGT
  CCCCGAGGGGCCTGGCTTCTGTCCCCAGAGGACTCCAGAGGGTGACCGGGCATTCAGAATTGGCCAGGCC
  AGCACGCTCGGCATCCCCACGTGGGAACTCTTTCCAGAAGGCCACTTTCCTGAGAACTTCTCCTTGCTGA
  TCACCTTGCGGGGACAGCCAGCCAATCAGTCTGTCCTGCTGTCCATTTATGATGAAAGGGGTGCCCGGCA
  GTTGGGCCTGGCACTGGGGCCAGCGCTGGGTCTCCTAGGTGACCCCTTCCGCCCCCTCCCCCAGCAGGTC
  AACCTCACAGATGGCAGGTGGCACCGTGTGGCCGTCAGCATAGATGGTGAGATGGTGACCCTGGTAGCTG
  ACTGTGAAGCTCAGCCCCCTGTTTTGGGCCATGGCCCCCGCTTCATCAGCATAGCTGGACTCACTGTGCT
  GGGGACCCAGGACCTTGGGGAAAAGACTTTCGAGGGAGACATTCAGGAGCTGCTGATAAGCCCAGATCCT
  CAGGCTGCCTTCCAGGCTTGTGAGCGGTACCTCCCCGACTGTGACAACCTGGCACCGGCAGCCACAGTGG
  CTCCCCAGGGTGAACCAGAAACCCCTCGTCCTCGGCGGAAGGGGAAGGGAAAAGGGAGGAAGAAAGGGCG
  AGGTCGCAAGGGGAAGGGCAGGAAAAAGAACAAGGAAATTTGGACCTCAAGTCCACCTCCTGACTCCGCA
  GAGAACCAGACCTCCACTGACATCCCCAAGACAGAGACTCCAGCTCCAAATCTGCCTCCGACCCCCACGC
  CTTTGGTCGTCACCTCCACTGTGACTACTGGACTCAATGCCACGATCCTAGAGGGGAGCTTGGACCCTGA
  CAGTGGAACCGAGCTGGGGACCCTGGAGACCAAGGCAGCCAGGGAGGATGAAGAAGGAGATGATTCCACC
  ATGGGCCCTGACTTCCGGGCAGCAGAATATCCATCTCGGACTCAGTTCCAGATCTTTCCTGGTGCTGGAG
  AGAAAGGAGCAAAAGGAGAGCCCGCAGTGATTGAAAAGGGGCAGCAGTTTGAGGGACCTCCAGGAGCCCC
  AGGACCCCAAGGGGTGGTTGGCCCCTCAGGCCCTCCCGGCCCCCCAGGATTCCCTGGCGACCCTGGTCCA
  CCGGGCCCTGCTGGCCTCCCAGGAATCCCCGGCATTGATGGGATCCGAGGCCCACCGGGCACTGTGATCA
  TGATGCCGTTCCAGTTTGCAGGCGGCTCCTTTAAAGGCCCCCCAGTCTCATTCCAGCAGGCCCAGGCTCA
  GGCAGTTCTGCAGCAGACTCAGCTCTCTATGAAAGGCCCCCCTGGTCCAGTGGGGCTCACTGGGCGCCCA
  GGCCCTGTGGGTCTCCCCGGGCATCCAGGTCTGAAAGGAGAGGAGGGAGCAGAAGGGCCACAGGGTCCCC
  GAGGCCTGCAGGGACCTCATGGACCCCCTGGCCGAGTGGGCAAGATGGGCCGCCCTGGAGCAGATGGAGC
  TCGGGGCCTCCCAGGGGACACTGGACCTAAGGGTGATCGTGGCTTCGATGGCCTCCCTGGGCTGCCTGGT
  GAGAAGGGCCAAAGGGGTGACTTTGGCCATGTGGGGCAACCCGGTCCCCCAGGAGAGGATGGTGAGAGGG
  GAGCAGAGGGACCTCCAGGGCCCACTGGCCAGGCTGGGGAGCCGGGTCCACGAGGACTGCTTGGCCCCAG
  AGGCTCTCCTGGCCCCACGGGTCGCCCGGGTGTGACTGGAATTGATGGTGCTCCTGGTGCCAAAGGCAAT
  GTGGGTCCTCCAGGAGAACCAGGCCCTCCGGGACAGCAGGGAAACCATGGGTCCCAGGGACTCCCCGGTC
  CCCAGGGACTCATTGGCACTCCTGGGGAGAAGGGTCCCCCTGGAAACCCAGGAATTCCAGGCCTCCCAGG
  ATCCGATGGCCCTCTGGGTCACCCAGGACATGAGGGCCCCACGGGAGAGAAAGGGGCTCAGGGTCCACCA
  GGGTCGGCAGGCCCTCCGGGCTATCCTGGACCTCGGGGAGTGAAGGGCACTTCAGGCAACCGGGGCCTCC
  AGGGGGAGAAAGGCGAGAAGGGAGAGGACGGCTTCCCAGGCTTCAAGGGCGATGTGGGGCTCAAAGGTGA
  TCAGGGGAAACCCGGAGCTCCAGGTCCCCGGGGAGAGGATGGTCCTGAGGGGCCGAAGGGGCAGGCGGGG
  CAGGCTGGCGAGGAGGGGCCCCCAGGCTCAGCTGGGGAGAAGGGCAAGCTTGGGGTGCCAGGCCTCCCAG
  GTTATCCAGGACGCCCTGGACCTAAGGGATCTATTGGATTTCCCGGTCCCCTGGGACCCATAGGAGAGAA
  AGGGAAGTCGGGAAAGACAGGGCAGCCAGGCCTGGAAGGAGAGCGGGGACCACCAGGTTCCCGTGGAGAG
  AGGGGGCAACCGGGTGCCACAGGGCAACCAGGCCCCAAGGGCGATGTGGGCCAGGATGGAGCCCCTGGGA
  TCCCTGGAGAAAAGGGCCTCCCTGGTCTGCAAGGCCCTCCAGGATTCCCTGGGCCAAAGGGCCCCCCTGG
  TCACCAAGGTAAAGATGGGCGACCAGGGCACCCTGGACAGAGAGGAGAACTGGGCTTCCAAGGTCAGACA
  GGCCCGCCTGGACCAGCTGGTGTCTTAGGCCCTCAGGGAAAGACAGGAGAAGTGGGACCTCTAGGTGAAA
  GGGGGCCTCCAGGCCCCCCTGGACCTCCTGGTGAACAAGGTCTTCCTGGCCTGGAAGGCAGAGAGGGGGC
  CAAGGGGGAACTGGGACCACCAGGACCCCTTGGGAAAGAAGGGCCAGCTGGACTCAGGGGCTTTCCCGGC
  CCCAAAGGGGGCCCTGGGGACCCGGGACCTACTGGCTTAAAGGGTGATAAGGGCCCCCCAGGGCCTGTGG
  GGGCCAATGGCTCCCCTGGTGAGCGCGGTCCTTTGGGCCCAGCAGGAGGCATTGGACTTCCTGGCCAAAG
  TGGCAGCGAAGGCCCCGTTGGCCCTGCAGGCAAGAAGGGGTCCCGGGGAGAACGTGGCCCCCCTGGCCCC
  ACTGGCAAAGATGGGATCCCAGGGCCCCTGGGGCCTCTGGGACCCCCTGGAGCTGCTGGGCCTTCTGGCG
  AGGAAGGGGACAAGGGGGATGTGGGTGCCCCCGGACACAAGGGGAGTAAAGGCGATAAAGGAGACGCGGG
  CCCACCTGGACAACCAGGGATACGGGGTCCTGCAGGACACCCAGGTCCCCCGGGAGCAGACGGGGCTCAG
  GGGCGCCGGGGACCCCCAGGCCTCTTTGGGCAGAAAGGAGATGACGGAGTCAGAGGCTTTGTGGGGGTGA
  TTGGCCCTCCTGGACTGCAGGGGCTGCCAGGCCCTCCGGGAGAGAAAGGGGAGGTCGGAGACGTCGGGTC
  CATGGGTCCCCATGGAGCTCCAGGTCCTCGGGGTCCCCAAGGCCCCACTGGATCAGAGGGCACTCCAGGG
  CTGCCTGGAGGAGTTGGTCAGCCAGGCGCCGTGGGTGAGAAGGGTGAGCGAGGGGACGCTGGAGACCCAG
  GGCCTCCAGGAGCCCCAGGCATCCCGGGGCCCAAGGGAGACATTGGTGAAAAGGGGGACTCAGGCCCATC
  TGGAGCTGCTGGACCCCCAGGCAAGAAAGGTCCCCCTGGAGAGGATGGAGCCAAAGGGAGCGTGGGCCCC
  ACGGGGCTGCCCGGAGATCTAGGGCCCCCAGGAGACCCTGGAGTTTCAGGCATAGATGGTTCCCCAGGGG
  AGAAGGGAGACCCTGGTGATGTTGGGGGACCGGGTCCGCCTGGAGCTTCTGGGGAGCCCGGCGCCCCCGG
  GCCCCCCGGCAAGAGGGGTCCTTCAGGCCACATGGGTCGAGAAGGCAGAGAAGGGGAGAAAGGTGCCAAG
  GGGGAGCCAGGTCCTGATGGGCCCCCAGGGAGGACGGGTCCAATGGGGGCTAGAGGGCCCCCTGGACGTG
  TGGGGCCTGAGGGTCTTCGAGGGATCCCTGGCCCTGTGGGTGAACCAGGCCTCCTGGGAGCCCCTGGACA
  GATGGGCCCTCCTGGCCCCCTGGGGCCCTCTGGCCTCCCAGGGCTGAAGGGAGACACTGGCCCCAAGGGG
  GAAAAGGGCCACATTGGATTGATCGGTCTCATTGGCCCCCCGGGAGAAGCTGGTGAGAAAGGAGATCAGG
  GGTTGCCAGGCGTGCAGGGACCCCCTGGTCCCAAGGGAGACCCTGGTCCCCCTGGTCCCATTGGCTCTCT
  GGGCCACCCTGGGCCCCCAGGTGTGGCGGGCCCTCTAGGACAGAAAGGCTCAAAAGGGTCTCCGGGGTCC
  ATGGGCCCCCGTGGAGACACTGGACCTGCAGGCCCACCAGGCCCCCCGGGTGCCCCTGCCGAGCTGCATG
  GGCTGCGCAGGCGCCGGCGCTTCGTCCCAGTCCCGCTTCCAGTCGTGGAGGGCGGCCTGGAGGAGGTGCT
  GGCCTCGCTCACATCGCTGAGCTTGGAGCTGGAGCAGCTGCGGCGTCCTCCCGGCACTGCGGAGCGCCCG
  GGCCTCGTGTGCCACGAGCTGCACCGCAACCACCCGCACCTGCCTGATGGGGAATACTGGATTGACCCCA
  ACCAGGGCTGCGCGCGGGACTCGTTCAGGGTTTTTTGCAACTTCACGGCGGGAGGAGAGACCTGCCTCTA
  TCCCGACAAGAAGTTTGAGATCGTGAAATTGGCCTCCTGGTCCAAGGAAAAGCCTGGAGGCTGGTATAGC
  ACATTCCGTCGAGGGAAGAAGTTCTCCTACGTGGACGCCGACGGGTCCCCAGTGAATGTCGTGCAGCTGA
  ACTTCCTGAAACTGCTGAGTGCCACAGCTCGCCAGAACTTCACCTACTCCTGCCAGAATGCAGCTGCCTG
  GCTGGACGAAGCCACGGGTGACTACAGCCACTCCGCCCGCTTCCTTGGCACCAATGGAGAGGAGCTGTCT
  TTCAACCAGACGACAGCAACCACTGTCAGCGTCCCCCAGGATGGCTGCCGGCTCCGGAAAGGACAGACGA
  AGACCCTTTTCGAATTCAGCTCTTCTCGAGCGGGATTTCTGCCCCTGTGGGATGTGGCGGCCACTGACTT
  TGGCCAGACGAACCAAAAGTTTGGGTTTGAACTGGGCCCCGTCTGCTTCAGCAGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC222823 representing NM_015719
  Red=Cloning site Green=Tags(s)
  
  MGNRRDLGQPRAGLCLLLAALQLLPGTQADPVDVLKALGVQGGQAGVPEGPGFCPQRTPEGDRAFRIGQA
  STLGIPTWELFPEGHFPENFSLLITLRGQPANQSVLLSIYDERGARQLGLALGPALGLLGDPFRPLPQQV
  NLTDGRWHRVAVSIDGEMVTLVADCEAQPPVLGHGPRFISIAGLTVLGTQDLGEKTFEGDIQELLISPDP
  QAAFQACERYLPDCDNLAPAATVAPQGEPETPRPRRKGKGKGRKKGRGRKGKGRKKNKEIWTSSPPPDSA
  ENQTSTDIPKTETPAPNLPPTPTPLVVTSTVTTGLNATILEGSLDPDSGTELGTLETKAAREDEEGDDST
  MGPDFRAAEYPSRTQFQIFPGAGEKGAKGEPAVIEKGQQFEGPPGAPGPQGVVGPSGPPGPPGFPGDPGP
  PGPAGLPGIPGIDGIRGPPGTVIMMPFQFAGGSFKGPPVSFQQAQAQAVLQQTQLSMKGPPGPVGLTGRP
  GPVGLPGHPGLKGEEGAEGPQGPRGLQGPHGPPGRVGKMGRPGADGARGLPGDTGPKGDRGFDGLPGLPG
  EKGQRGDFGHVGQPGPPGEDGERGAEGPPGPTGQAGEPGPRGLLGPRGSPGPTGRPGVTGIDGAPGAKGN
  VGPPGEPGPPGQQGNHGSQGLPGPQGLIGTPGEKGPPGNPGIPGLPGSDGPLGHPGHEGPTGEKGAQGPP
  GSAGPPGYPGPRGVKGTSGNRGLQGEKGEKGEDGFPGFKGDVGLKGDQGKPGAPGPRGEDGPEGPKGQAG
  QAGEEGPPGSAGEKGKLGVPGLPGYPGRPGPKGSIGFPGPLGPIGEKGKSGKTGQPGLEGERGPPGSRGE
  RGQPGATGQPGPKGDVGQDGAPGIPGEKGLPGLQGPPGFPGPKGPPGHQGKDGRPGHPGQRGELGFQGQT
  GPPGPAGVLGPQGKTGEVGPLGERGPPGPPGPPGEQGLPGLEGREGAKGELGPPGPLGKEGPAGLRGFPG
  PKGGPGDPGPTGLKGDKGPPGPVGANGSPGERGPLGPAGGIGLPGQSGSEGPVGPAGKKGSRGERGPPGP
  TGKDGIPGPLGPLGPPGAAGPSGEEGDKGDVGAPGHKGSKGDKGDAGPPGQPGIRGPAGHPGPPGADGAQ
  GRRGPPGLFGQKGDDGVRGFVGVIGPPGLQGLPGPPGEKGEVGDVGSMGPHGAPGPRGPQGPTGSEGTPG
  LPGGVGQPGAVGEKGERGDAGDPGPPGAPGIPGPKGDIGEKGDSGPSGAAGPPGKKGPPGEDGAKGSVGP
  TGLPGDLGPPGDPGVSGIDGSPGEKGDPGDVGGPGPPGASGEPGAPGPPGKRGPSGHMGREGREGEKGAK
  GEPGPDGPPGRTGPMGARGPPGRVGPEGLRGIPGPVGEPGLLGAPGQMGPPGPLGPSGLPGLKGDTGPKG
  EKGHIGLIGLIGPPGEAGEKGDQGLPGVQGPPGPKGDPGPPGPIGSLGHPGPPGVAGPLGQKGSKGSPGS
  MGPRGDTGPAGPPGPPGAPAELHGLRRRRRFVPVPLPVVEGGLEEVLASLTSLSLELEQLRRPPGTAERP
  GLVCHELHRNHPHLPDGEYWIDPNQGCARDSFRVFCNFTAGGETCLYPDKKFEIVKLASWSKEKPGGWYS
  TFRRGKKFSYVDADGSPVNVVQLNFLKLLSATARQNFTYSCQNAAAWLDEATGDYSHSARFLGTNGEELS
  FNQTTATTVSVPQDGCRLRKGQTKTLFEFSSSRAGFLPLWDVAATDFGQTNQKFGFELGPVCFSS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015719
• ORF Size: 5235 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_015719.1, NM_015719.2, NM_015719.3, NP_056534.1, NP_056534.2
• RefSeq Size: 6200
• RefSeq ORF: 5238
• Locus ID: 50509
• Protein Pathways: ECM-receptor interaction, Focal adhesion
• MW: 172.05 kDa
• Gene Summary: This gene encodes an alpha chain for one of the low abundance fibrillar collagens. Fibrillar collagen molecules are trimers that can be composed of one or more types of alpha chains. Type V collagen is found in tissues containing type I collagen and appears to regulate the assembly of heterotypic fibers composed of both type I and type V collagen. This gene product is closely related to type XI collagen and it is possible that the collagen chains of types V and XI constitute a single collagen type with tissue-specific chain combinations. Mutations in this gene are thought to be responsible for the symptoms of a subset of patients with Ehlers-Danlos syndrome type III. Messages of several sizes can be detected in northern blots but sequence information cannot confirm the identity of the shorter messages. [provided by RefSeq, Jul 2008]