UNG (NM_080911) Human Tagged ORF Clone
CAT#: RC222868
UNG (Myc-DDK-tagged)-Human uracil-DNA glycosylase (UNG), transcript variant 2
"NM_080911" in other vectors (6)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | UNG |
Synonyms | DGU; HIGM4; HIGM5; UDG; UNG1; UNG2; UNG15 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC222868 representing NM_080911
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATCGGCCAGAAGACGCTCTACTCCTTTTTCTCCCCCAGCCCCGCCAGGAAGCGACACGCCCCCAGCC CCGAGCCGGCCGTCCAGGGGACCGGCGTGGCTGGGGTGCCTGAGGAAAGCGGAGATGCGGCGGCCATCCC AGCCAAGAAGGCCCCGGCTGGGCAGGAGGAGCCTGGGACGCCGCCCTCCTCGCCGCTGAGTGCCGAGCAG TTGGACCGGATCCAGAGGAACAAGGCCGCGGCCCTGCTCAGACTCGCGGCCCGCAACGTGCCCGTGGGCT TTGGAGAGAGCTGGAAGAAGCACCTCAGCGGGGAGTTCGGGAAACCGTATTTTATCAAGCTAATGGGATT TGTTGCAGAAGAAAGAAAGCATTACACTGTTTATCCACCCCCACACCAAGTCTTCACCTGGACCCAGATG TGTGACATAAAAGATGTGAAGGTTGTCATCCTGGGACAGGATCCATATCATGGACCTAATCAAGCTCACG GGCTCTGCTTTAGTGTTCAAAGGCCTGTTCCGCCTCCGCCCAGTTTGGAGAACATTTATAAAGAGTTGTC TACAGACATAGAGGATTTTGTTCATCCTGGCCATGGAGATTTATCTGGGTGGGCCAAGCAAGGTGTTCTC CTTCTCAACGCTGTCCTCACGGTTCGTGCCCATCAAGCCAACTCTCATAAGGAGCGAGGCTGGGAGCAGT TCACTGATGCAGTTGTGTCCTGGCTAAATCAGAACTCGAATGGCCTTGTTTTCTTGCTCTGGGGCTCTTA TGCTCAGAAGAAGGGCAGTGCCATTGATAGGAAGCGGCACCATGTACTACAGACGGCTCATCCCTCCCCT TTGTCAGTGTATAGAGGGTTCTTTGGATGTAGACACTTTTCAAAGACCAATGAGCTGCTGCAGAAGTCTG GCAAGAAGCCCATTGACTGGAAGGAGCTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC222868 representing NM_080911
Red=Cloning site Green=Tags(s) MIGQKTLYSFFSPSPARKRHAPSPEPAVQGTGVAGVPEESGDAAAIPAKKAPAGQEEPGTPPSSPLSAEQ LDRIQRNKAAALLRLAARNVPVGFGESWKKHLSGEFGKPYFIKLMGFVAEERKHYTVYPPPHQVFTWTQM CDIKDVKVVILGQDPYHGPNQAHGLCFSVQRPVPPPPSLENIYKELSTDIEDFVHPGHGDLSGWAKQGVL LLNAVLTVRAHQANSHKERGWEQFTDAVVSWLNQNSNGLVFLLWGSYAQKKGSAIDRKRHHVLQTAHPSP LSVYRGFFGCRHFSKTNELLQKSGKKPIDWKEL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_080911 |
ORF Size | 939 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_080911.1, NM_080911.2, NP_550433.1 |
RefSeq Size | 2053 bp |
RefSeq ORF | 942 bp |
Locus ID | 7374 |
Cytogenetics | 12q24.11 |
Domains | UDG |
Protein Families | Druggable Genome, Stem cell - Pluripotency |
Protein Pathways | Base excision repair, Primary immunodeficiency |
MW | 34.5 kDa |
Gene Summary | 'This gene encodes one of several uracil-DNA glycosylases. One important function of uracil-DNA glycosylases is to prevent mutagenesis by eliminating uracil from DNA molecules by cleaving the N-glycosylic bond and initiating the base-excision repair (BER) pathway. Uracil bases occur from cytosine deamination or misincorporation of dUMP residues. Alternative promoter usage and splicing of this gene leads to two different isoforms: the mitochondrial UNG1 and the nuclear UNG2. The UNG2 term was used as a previous symbol for the CCNO gene (GeneID 10309), which has been confused with this gene, in the literature and some databases. [provided by RefSeq, Nov 2010]' |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC109875 | UNG (untagged)-Human uracil-DNA glycosylase (UNG), transcript variant 2 |
USD 660.00 |
|
RG222868 | UNG (GFP-tagged) - Human uracil-DNA glycosylase (UNG), transcript variant 2 |
USD 460.00 |
|
RC222868L1 | Lenti ORF clone of Human uracil-DNA glycosylase (UNG), transcript variant 2, Myc-DDK-tagged |
USD 768.00 |
|
RC222868L2 | Lenti ORF clone of Human uracil-DNA glycosylase (UNG), transcript variant 2, mGFP tagged |
USD 620.00 |
|
RC222868L3 | Lenti ORF clone of Human uracil-DNA glycosylase (UNG), transcript variant 2, Myc-DDK-tagged |
USD 620.00 |
|
RC222868L4 | Lenti ORF clone of Human uracil-DNA glycosylase (UNG), transcript variant 2, mGFP tagged |
USD 620.00 |
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