UNG (NM_080911) Human Untagged Clone

CAT#: SC109875

UNG (untagged)-Human uracil-DNA glycosylase (UNG), transcript variant 2


  "NM_080911" in other vectors (6)

Reconstitution Protocol

USD 660.00

In Stock*

Size
    • 10 ug

Product Images

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol UNG
Synonyms DGU; HIGM4; HIGM5; UDG; UNG1; UNG2; UNG15
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>NCBI ORF sequence for NM_080911, the custom clone sequence may differ by one or more nucleotides


ATGATCGGCCAGAAGACGCTCTACTCCTTTTTCTCCCCCAGCCCCGCCAGGAAGCGACACGCCCCCAGCC
CCGAGCCGGCCGTCCAGGGGACCGGCGTGGCTGGGGTGCCTGAGGAAAGCGGAGATGCGGCGGCCATCCC
AGCCAAGAAGGCCCCGGCTGGGCAGGAGGAGCCTGGGACGCCGCCCTCCTCGCCGCTGAGTGCCGAGCAG
TTGGACCGGATCCAGAGGAACAAGGCCGCGGCCCTGCTCAGACTCGCGGCCCGCAACGTGCCCGTGGGCT
TTGGAGAGAGCTGGAAGAAGCACCTCAGCGGGGAGTTCGGGAAACCGTATTTTATCAAGCTAATGGGATT
TGTTGCAGAAGAAAGAAAGCATTACACTGTTTATCCACCCCCACACCAAGTCTTCACCTGGACCCAGATG
TGTGACATAAAAGATGTGAAGGTTGTCATCCTGGGACAGGATCCATATCATGGACCTAATCAAGCTCACG
GGCTCTGCTTTAGTGTTCAAAGGCCTGTTCCGCCTCCGCCCAGTTTGGAGAACATTTATAAAGAGTTGTC
TACAGACATAGAGGATTTTGTTCATCCTGGCCATGGAGATTTATCTGGGTGGGCCAAGCAAGGTGTTCTC
CTTCTCAACGCTGTCCTCACGGTTCGTGCCCATCAAGCCAACTCTCATAAGGAGCGAGGCTGGGAGCAGT
TCACTGATGCAGTTGTGTCCTGGCTAAATCAGAACTCGAATGGCCTTGTTTTCTTGCTCTGGGGCTCTTA
TGCTCAGAAGAAGGGCAGTGCCATTGATAGGAAGCGGCACCATGTACTACAGACGGCTCATCCCTCCCCT
TTGTCAGTGTATAGAGGGTTCTTTGGATGTAGACACTTTTCAAAGACCAATGAGCTGCTGCAGAAGTCTG
GCAAGAAGCCCATTGACTGGAAGGAGCTGTGA


>OriGene 5' read for NM_080911 unedited
CATTCGCGCTCGAGGCCACAGCGCGGTTCTAGCCTCGCCGGTTCCCGGGTGGCGCGCGTT
CGCTGCCTCCTCAGCTCCAGGATGATCGGCCAGAAGACGCTCTACTCCTTTTTCTCCCCC
AGCCCCGCCAGGAAGCGACACGCCCCCAGCCCCGAGCCGGCCGTCCAGGGGACTCGCGTG
GCTGGGGTGCCTGAGGAAAGCTGAGATGCGGCGGCCATCCCAGCCAAGAAGGCCCCGGCT
GGGCAGGAGGAGCCTGGGACTCCGCCCTCCTCGCCGCTGAGTGCCGAGCAGTTGGACCGG
ATCCAGAGGAACAAGGCCGCGGCCCTGCTCAGACTCGCGGCCCGCAACGTGCCCGTGGGC
TTTGGAGAGAGCTGGAAGAAGCACCTCAGCGGGGAGTTCGGGAAACCGTATTTTATCAAG
CTAATGGGATTTGTTGCAGAAGACAGAAAGCATTACACTGTTTATCCACCCCCACACCAA
GTCTTCACCTGGACCCAGATGTGTGACATAAAAGATGTGAAGGTTGTCATCCTGGGACAG
GATCCATATCATGGACCTAATCAAGCTCACGGGCTCTGCTTTAGTGTTCAAAGGCCTGTT
CCGCCTCCGCCCAGTTTGGACGAACATTATAAAGAGTTGTCTACAGACATAGAGGATTTT
GTTCATTCTGACCATGGAGATTTATCTGGGTGGGCCCAGCAAAGTGTTCTGCTTCTCAAC
GCTGTCCTCACGGGTCGGGCCCTTCAAGCCCACTCCCATTAAGAGCGAGCGTGGGACCAG
TTCCTGGAGCAGTTGTGTACCTGGCTAATCAGAACTTCGAGGCCCTTGTTTCCTTGTCCG
GGGCTCTTATGCTCAAAAAAGGCCGTGCCTTTGGTGGGAACGCGCC
>OriGene 3' read for NM_080911 unedited
AGTGTTCAAAATCCATTTAAGGCTCGCGTAAAACCACTCTAACCCGNCTAAAGCAAAAAC
AGCATTGCTAACAAGATCAAGGACGGAGGTGCAGACCCAATAAAGACCGTCTCTTAAAAA
AAGGACTGGNGAAAAATATATATATATATATCTGCATGAGTACTTCTTAAAAAACTAAAA
CTTTCCAGTGTTGCAGGAAAAAATATTTTTTTTGATTTCAGTCGGTTAAAAAAAACAGTA
CTTGGCAGTCTGTGCTGCCTTAAAAACCCCAACTGGACATAACCCTAGGAGAAAATCACC
CCACCCCATAAAGACAAAATTCCCTTTTCTCCTCTTCCTTGTTCAAATTCCACTGCTTAT
TCCACCAACACCCAAGGAATAACAAGACCCAGCTTACTTCTCTCGGTCTCTTATCTGATA
AAGCTCACAGCAATCTTAACCTCTACTACAATCAGGAAATTGAGCCCTAATTTCTGGGCC
AGATTCTGGAAAGTTGCAAAACATCCTTATGCTGCGAGGGGCCTGAGGACTCCTTCAAAG
ATCAAGGGGGCAGTGGAACTGGCAGAGACTGGCATGCCTGCCCTGCAGGCTGAGACAAGG
GACCTTTCTAAGCAAAAACACCCAACATCTGTCACTGAAAGTCTAACCAGGCCAAACCAA
GGAGCAGCAGGCGCCCAACTAGGCTGTGCCCTGAAAGTGCACATCTTCCCTGTTTCACAT
AAGGCCAGGGAGATAAAAAAGCCAACTGAGTATTTGACCTCATCTGTCCTCTGGATATTT
TAAGCCCAAACCCTTGTTGCAGAAAAAGAACTTTGGTCAAAGGGTACCCTTGTTTGTTGG
GGTTGGAAACAAGTGCCTTTCCGGACAGCCTGGTTAATGGCTGCTTTCGGGAAACTTTTC
CCCTTTAGCCGAGACCTTAAAGATTAATAG
Restriction Sites NotI-NotI     
ACCN NM_080911
Insert Size 2300 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_080911.1, NP_550433.1
RefSeq Size 2053 bp
RefSeq ORF 942 bp
Locus ID 7374
Cytogenetics 12q24.11
Domains UDG
Protein Families Druggable Genome, Stem cell - Pluripotency
Protein Pathways Base excision repair, Primary immunodeficiency
Gene Summary 'This gene encodes one of several uracil-DNA glycosylases. One important function of uracil-DNA glycosylases is to prevent mutagenesis by eliminating uracil from DNA molecules by cleaving the N-glycosylic bond and initiating the base-excision repair (BER) pathway. Uracil bases occur from cytosine deamination or misincorporation of dUMP residues. Alternative promoter usage and splicing of this gene leads to two different isoforms: the mitochondrial UNG1 and the nuclear UNG2. The UNG2 term was used as a previous symbol for the CCNO gene (GeneID 10309), which has been confused with this gene, in the literature and some databases. [provided by RefSeq, Nov 2010]'
Transcript Variant: This variant (2) uses an upstream promoter and an alternate splice site for exon 1B when compared to variant 1. It encodes the nuclear UNG2 isoform, which has a different N-terminal sequence than the UNG1 isoform.

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