C1R (NM_001733) Human Tagged ORF Clone

CAT#: RC223007

C1R (Myc-DDK-tagged)-Human complement component 1, r subcomponent (C1R)

Product Images

Western blot validation of overexpression lysate (Cat# LY400654) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC223007 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: C1R
• Synonyms: EDSPD1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC223007 representing NM_001733
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGGCTCTTGTACCTCCTGGTGCCGGCCCTGTTCTGCAGGGCAGGAGGCTCCATTCCCATCCCTCAGA
  AGTTATTTGGGGAGGTGACTTCCCCTCTGTTCCCCAAGCCTTACCCCAACAACTTTGAAACAACCACTGT
  GATCACAGTCCCCACGGGATACAGGGTGAAGCTCGTCTTCCAGCAGTTTGACCTGGAGCCTTCTGAAGGC
  TGCTTCTATGATTATGTCAAGATCTCTGCTGATAAGAAAAGCCTGGGGAGGTTCTGTGGGCAACTGGGTT
  CTCCACTGGGCAACCCCCCGGGAAAGAAGGAATTTATGTCCCAAGGGAACAAGATGCTGCTGACCTTCCA
  CACAGACTTCTCCAACGAGGAGAATGGGACCATCATGTTCTACAAGGGCTTCCTGGCCTACTACCAAGCT
  GTGGACCTTGATGAATGTGCTTCCCGGAGCAAATCAGGGGAGGAGGATCCCCAGCCCCAGTGCCAGCACC
  TGTGTCACAACTACGTTGGAGGCTACTTCTGTTCCTGCCGTCCAGGCTATGAGCTTCAGGAAGACAGGCA
  TTCCTGCCAGGCTGAGTGCAGCAGCGAGCTGTACACGGAGGCATCAGGCTACATCTCCAGCCTGGAGTAC
  CCTCGGTCCTACCCCCCTGACCTGCGCTGCAACTACAGCATCCGGGTGGAGCGGGGCCTCACCCTGCACC
  TCAAGTTCCTGGAGCCTTTTGATATTGATGACCACCAGCAAGTACACTGCCCCTATGACCAGCTACAGAT
  CTATGCCAACGGGAAGAACATTGGCGAGTTCTGTGGGAAGCAAAGGCCCCCCGACCTCGACACCAGCAGC
  AATGCTGTGGATCTGCTGTTCTTCACAGATGAGTCGGGGGACAGCCGGGGCTGGAAGCTGCGCTACACCA
  CCGAGATCATCAAGTGCCCCCAGCCCAAGACCCTAGACGAGTTCACCATCATCCAGAACCTGCAGCCTCA
  GTACCAGTTCCGTGACTACTTCATTGCTACCTGCAAGCAAGGCTACCAGCTCATAGAGGGGAACCAGGTG
  CTGCATTCCTTCACAGCTGTCTGCCAGGATGATGGCACGTGGCATCGTGCCATGCCCAGATGCAAGATCA
  AGGACTGTGGGCAGCCCCGAAACCTGCCTAATGGTGACTTCCGTTACACCACCACAATGGGAGTGAACAC
  CTACAAGGCCCGTATCCAGTACTACTGCCATGAGCCATATTACAAGATGCAGACCAGAGCTGGCAGCAGG
  GAGTCTGAGCAAGGGGTGTACACCTGCACAGCACAGGGCATTTGGAAGAATGAACAGAAGGGAGAGAAGA
  TTCCTCGGTGCTTGCCAGTGTGTGGGAAGCCCGTGAACCCCGTGGAACAGAGGCAGCGCATCATCGGAGG
  GCAAAAAGCCAAGATGGGCAACTTCCCCTGGCAGGTGTTCACCAACATCCACGGGCGCGGGGGCGGGGCC
  CTGCTGGGCGACCGCTGGATCCTCACAGCTGCCCACACCCTGTATCCCAAGGAACACGAAGCGCAAAGCA
  ACGCCTCTTTGGATGTGTTCCTGGGCCACACAAATGTGGAAGAGCTCATGAAGCTAGGAAATCACCCCAT
  CCGCAGGGTCAGCGTCCACCCGGACTACCGTCAGGATGAGTCCTACAATTTTGAGGGGGACATCGCCCTG
  CTGGAGCTGGAAAATAGTGTCACCCTGGGTCCCAACCTCCTCCCCATCTGCCTCCCTGACAACGATACCT
  TCTACGACCTGGGCTTGATGGGCTATGTCAGTGGCTTCGGGGTCATGGAGGAGAAGATTGCTCATGACCT
  CAGGTTTGTCCGTCTGCCCGTAGCTAATCCACAGGCCTGTGAGAACTGGCTCCGGGGAAAGAATAGGATG
  GATGTGTTCTCTCAAAACATGTTCTGTGCTGGACACCCATCTCTAAAGCAGGACGCCTGCCAGGGGGATA
  GTGGGGGCGTTTTTGCAGTAAGGGACCCGAACACTGATCGCTGGGTGGCCACGGGCATCGTGTCCTGGGG
  CATCGGGTGCAGCAGGGGCTATGGCTTCTACACCAAAGTGCTCAACTACGTGGACTGGATCAAGAAAGAG
  ATGGAGGAGGAGGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC223007 representing NM_001733
  Red=Cloning site Green=Tags(s)
  
  MWLLYLLVPALFCRAGGSIPIPQKLFGEVTSPLFPKPYPNNFETTTVITVPTGYRVKLVFQQFDLEPSEG
  CFYDYVKISADKKSLGRFCGQLGSPLGNPPGKKEFMSQGNKMLLTFHTDFSNEENGTIMFYKGFLAYYQA
  VDLDECASRSKSGEEDPQPQCQHLCHNYVGGYFCSCRPGYELQEDRHSCQAECSSELYTEASGYISSLEY
  PRSYPPDLRCNYSIRVERGLTLHLKFLEPFDIDDHQQVHCPYDQLQIYANGKNIGEFCGKQRPPDLDTSS
  NAVDLLFFTDESGDSRGWKLRYTTEIIKCPQPKTLDEFTIIQNLQPQYQFRDYFIATCKQGYQLIEGNQV
  LHSFTAVCQDDGTWHRAMPRCKIKDCGQPRNLPNGDFRYTTTMGVNTYKARIQYYCHEPYYKMQTRAGSR
  ESEQGVYTCTAQGIWKNEQKGEKIPRCLPVCGKPVNPVEQRQRIIGGQKAKMGNFPWQVFTNIHGRGGGA
  LLGDRWILTAAHTLYPKEHEAQSNASLDVFLGHTNVEELMKLGNHPIRRVSVHPDYRQDESYNFEGDIAL
  LELENSVTLGPNLLPICLPDNDTFYDLGLMGYVSGFGVMEEKIAHDLRFVRLPVANPQACENWLRGKNRM
  DVFSQNMFCAGHPSLKQDACQGDSGGVFAVRDPNTDRWVATGIVSWGIGCSRGYGFYTKVLNYVDWIKKE
  MEEED
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001733
• ORF Size: 2115 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001733.1, NM_001733.2, NM_001733.3, NM_001733.4, NP_001724.3
• RefSeq Size: 2526 bp
• RefSeq ORF: 2118 bp
• Locus ID: 715
• Cytogenetics: 12p13.31
• Domains: CCP, CUB, Tryp_SPc, EGF_CA, EGF
• Protein Families: Druggable Genome, Protease
• Protein Pathways: Complement and coagulation cascades, Systemic lupus erythematosus
• MW: 80 kDa
• Gene Summary: 'This gene encodes a member of the peptidase S1 protein family. The encoded protein is a proteolytic subunit in the complement system C1 complex. The complement system acts as a mediator in the innate immune response by ultimately triggering phagocytosis, inflammation, and rupturing the bacterial cell wall. Mutations in this gene are associated with Ehlers-Danlos Syndrome. [provided by RefSeq, Dec 2018]'