IFT43 (NM_001102564) Human Tagged ORF Clone

CAT#: RC223029

IFT43 (Myc-DDK-tagged)-Human chromosome 14 open reading frame 179 (C14orf179), transcript variant 2

Product Images

Western blot validation of overexpression lysate (Cat# LY420159) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC223029 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: IFT43
• Synonyms: C14orf179; CED3; RP81; SRTD18
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC223029 representing NM_001102564
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGATTTGCTCGACTTGGACGAGGAGCTTCGCTACAGCTTGGCTACCTCCAGGGCCAAGATGGGTC
  GCCGAGCTCAACAGGAGTCAGCGCAGGCCGAGAATCACCTCAATGGCAAGAATTCCTCTTTGACTCTGAC
  TGGAGAGACTTCCTCTGCTAAATTACCTCGCTGCCGACAGGGAGGCTGGGCAGGTGATTCCGTGAAGGCT
  TCGAAGTTTAGGAGGAAGGCTTCTGAAGAAATAGAAGATTTCCGCCTCAGACCACAGAGCCTGAATGGAT
  CAGATTATGGAGGAGATATTCCTATCATTCCGGATCTGGAGGAAGTACAGGAAGAAGACTTTGTTTTGCA
  GGTGGCAGCCCCTCCCAGCATCCAGATAAAGCGGGTGATGACCTACCGTGACCTGGACAATGACCTCATG
  AAGTACTCAGCCATTCAGACACTGGATGGGGAGATCGACCTGAAACTCCTCACCAAAGTGCTCGCGCCGG
  AGCACGAAGTCCGGGAGGATGATGTCGGCTGGGACTGGGACCATCTGTTCACTGAGGTGTCCTCAGAGGT
  CCTCACTGAGTGGGACCCACTGCAGACGGAGAAGGAGGACCCTGCGGGGCAGGCCAGGCACACC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC223029 representing NM_001102564
  Red=Cloning site Green=Tags(s)
  
  MEDLLDLDEELRYSLATSRAKMGRRAQQESAQAENHLNGKNSSLTLTGETSSAKLPRCRQGGWAGDSVKA
  SKFRRKASEEIEDFRLRPQSLNGSDYGGDIPIIPDLEEVQEEDFVLQVAAPPSIQIKRVMTYRDLDNDLM
  KYSAIQTLDGEIDLKLLTKVLAPEHEVREDDVGWDWDHLFTEVSSEVLTEWDPLQTEKEDPAGQARHT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001102564
• ORF Size: 624 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001102564.1, NP_001096034.1
• RefSeq Size: 850
• RefSeq ORF: 627
• Locus ID: 112752
• MW: 23.3 kDa
• Gene Summary: This gene encodes a subunit of the intraflagellar transport complex A (IFT-A). IFT-A is a multiprotein complex that plays an important role in cilia assembly and maintenance by mediating retrograde ciliary transport. Mutations in this gene are a cause of cranioectodermal dysplasia-3 (CED3), also known as Sensenbrenner syndrome. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2011]