DMGDH (NM_013391) Human Tagged ORF Clone

CAT#: RC223089

DMGDH (Myc-DDK-tagged)-Human dimethylglycine dehydrogenase (DMGDH), nuclear gene encoding mitochondrial protein

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: DMGDH
• Synonyms: DMGDHD; ME2GLYDH
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC223089 representing NM_013391
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTCCGTCCCGGCGCGCAGCTGCTGCGGGGCCTCCTGCTGCGGAGCTGCCCGCTGCAGGGCTCCCCCG
  GGCGCCCGCGCTCTGTCTGCGGCCGGGAAGGAGAGGAAAAACCACCCTTATCTGCAGAAACACAATGGAA
  AGACAGAGCAGAAACAGTGATAATTGGAGGTGGCTGTGTTGGTGTGAGTCTGGCTTATCACCTGGCCAAA
  GCAGGGATGAAAGATGTGGTCCTGCTGGAGAAATCAGAGCTCACGGCTGGATCTACCTGGCACGCAGCAG
  GTTTAACAACTTACTTTCATCCTGGAATAAACTTGAAGAAAATACATTATGATAGCATCAAACTTTATGA
  GAAACTGGAAGAAGAAACTGGTCAGGTGGTGGGATTCCATCAGCCAGGTAGTATCAGACTTGCTACCACC
  CCTGTAAGGGTAGATGAATTTAAATATCAAATGACTCGGACTGGCTGGCATGCAACAGAACAGTATCTCA
  TTGAACCTGAAAAAATTCAAGAGATGTTCCCTTTACTCAACATGAATAAGGTTTTAGCTGGATTGTATAA
  TCCTGGAGATGGTCACATTGATCCTTATTCTCTAACTATGGCACTGGCTGCTGGGGCTAGGAAATGTGGT
  GCCCTTTTAAAATATCCTGCACCAGTAACTTCTCTGAAAGCCAGGTCAGATGGAACATGGGACGTTGAAA
  CACCACAGGGGTCTATGAGAGCAAATAGAATTGTGAATGCTGCAGGATTTTGGGCTCGTGAAGTAGGTAA
  AATGATTGGACTAGAACATCCTCTCATTCCGGTTCAACATCAATATGTTGTTACATCGACTATATCTGAA
  GTGAAAGCTTTGAAACGAGAACTGCCTGTGCTCCGTGACCTGGAAGGATCATATTATCTCCGACAGGAAA
  GGGATGGGCTTTTGTTTGGTCCATATGAAAGTCAAGAGAAAATGAAAGTTCAGGACTCCTGGGTCACCAA
  TGGAGTTCCTCCAGGTTTTGGAAAGGAACTCTTTGAGTCTGATCTAGATCGAATCATGGAACACATCAAA
  GCTGCCATGGAAATGGTTCCTGTCTTGAAAAAGGCTGACATCATCAATGTTGTCAATGGTCCTATCACGT
  ATTCTCCTGACATTCTGCCTATGGTGGGGCCCCATCAGGGGGTCAGAAACTACTGGGTGGCTATAGGCTT
  TGGATATGGCATAATCCACGCTGGTGGGGTAGGGAAATATCTCAGTGACTGGATCCTGCATGGAGAACCT
  CCTTTTGATCTGATAGAATTGGATCCTAATCGCTATGGCAAATGGACAACAACCCAGTACACTGAGGCCA
  AAGCAAGAGAATCATATGGATTCAACAATATTGTTGGTTATCCTAAAGAAGAACGGTTTGCTGGGAGGCC
  GACTCAACGAGTCAGTGGGCTCTATCAAAGGCTGGAGTCTAAGTGTTCCATGGGGTTCCATGCTGGCTGG
  GAGCAGCCGCACTGGTTCTACAAACCAGGCCAGGACACTCAGTACAGGCCAAGTTTTCGCCGCACAAACT
  GGTTTGAGCCTGTGGGCTCGGAGTATAAACAGGTTATGCAAAGAGTAGCGGTAACTGACCTATCACCATT
  TGGCAAGTTTAACATCAAAGGCCAAGATTCCATTAGACTACTGGACCATCTCTTTGCAAATGTCATTCCA
  AAGGTGGGTTTTACAAATATAAGTCACATGTTAACACCCAAGGGTCGAGTGTATGCTGAGCTGACTGTTT
  CTCACCAATCTCCTGGGGAGTTTCTTTTAATTACTGGCTCTGGATCAGAACTTCATGATCTTAGATGGAT
  TGAAGAAGAAGCAGTCAAAGGTGGATATGATGTTGAAATTAAAAACATAACTGATGAGCTTGGAGTTCTT
  GGAGTTGCTGGGCCACAGGCAAGAAAGGTCCTTCAGAAACTGACCTCTGAAGATCTTAGTGATGATGTTT
  TCAAGTTTCTTCAAACCAAGTCCTTAAAGGTTTCCAACATTCCTGTCACTGCTATTAGGATATCTTATAC
  TGGTGAGCTGGGTTGGGAGCTGTATCACAGAAGAGAAGATTCTGTGGCGCTGTATGACGCTATCATGAAT
  GCAGGCCAGGAGGAGGGAATCGACAATTTTGGAACCTATGCCATGAATGCCTTACGCCTGGAGAAAGCCT
  TCAGAGCCTGGGGGTTAGAGATGAACTGTGATACAAATCCTTTGGAAGCTGGACTGGAATATTTTGTGAA
  GTTAAATAAGCCAGCAGACTTCATAGGAAAGCAAGCACTGAAACAGATTAAAGCCAAGGGGCTGAAACGA
  AGACTGGTCTGCCTCACCTTGGCAACGGATGATGTTGATCCAGAGGGAAATGAAAGCATCTGGTACAATG
  GCAAGGTGGTTGGCAACACGACATCTGGAAGCTATAGCTACAGCATCCAGAAGAGTCTGGCTTTCGCATA
  TGTCCCTGTACAACTAAGTGAAGTGGGACAGCAAGTGGAAGTTGAACTATTAGGCAAAAATTACCCAGCA
  GTCATCATACAAGAACCTTTGGTATTGACCGAACCAACCAGAAACCGGCTTCAGAAAAAAGGTGGAAAGG
  ACAAAACT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC223089 representing NM_013391
  Red=Cloning site Green=Tags(s)
  
  MLRPGAQLLRGLLLRSCPLQGSPGRPRSVCGREGEEKPPLSAETQWKDRAETVIIGGGCVGVSLAYHLAK
  AGMKDVVLLEKSELTAGSTWHAAGLTTYFHPGINLKKIHYDSIKLYEKLEEETGQVVGFHQPGSIRLATT
  PVRVDEFKYQMTRTGWHATEQYLIEPEKIQEMFPLLNMNKVLAGLYNPGDGHIDPYSLTMALAAGARKCG
  ALLKYPAPVTSLKARSDGTWDVETPQGSMRANRIVNAAGFWAREVGKMIGLEHPLIPVQHQYVVTSTISE
  VKALKRELPVLRDLEGSYYLRQERDGLLFGPYESQEKMKVQDSWVTNGVPPGFGKELFESDLDRIMEHIK
  AAMEMVPVLKKADIINVVNGPITYSPDILPMVGPHQGVRNYWVAIGFGYGIIHAGGVGKYLSDWILHGEP
  PFDLIELDPNRYGKWTTTQYTEAKARESYGFNNIVGYPKEERFAGRPTQRVSGLYQRLESKCSMGFHAGW
  EQPHWFYKPGQDTQYRPSFRRTNWFEPVGSEYKQVMQRVAVTDLSPFGKFNIKGQDSIRLLDHLFANVIP
  KVGFTNISHMLTPKGRVYAELTVSHQSPGEFLLITGSGSELHDLRWIEEEAVKGGYDVEIKNITDELGVL
  GVAGPQARKVLQKLTSEDLSDDVFKFLQTKSLKVSNIPVTAIRISYTGELGWELYHRREDSVALYDAIMN
  AGQEEGIDNFGTYAMNALRLEKAFRAWGLEMNCDTNPLEAGLEYFVKLNKPADFIGKQALKQIKAKGLKR
  RLVCLTLATDDVDPEGNESIWYNGKVVGNTTSGSYSYSIQKSLAFAYVPVQLSEVGQQVEVELLGKNYPA
  VIIQEPLVLTEPTRNRLQKKGGKDKT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_013391
• ORF Size: 2598 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_013391.1, NM_013391.2, NM_013391.3, NP_037523.2
• RefSeq Size: 3104
• RefSeq ORF: 2601
• Locus ID: 29958
• Domains: DAO, GCV_T
• Protein Pathways: Glycine, serine and threonine metabolism, Metabolic pathways
• MW: 96.81 kDa
• Gene Summary: This gene encodes an enzyme involved in the catabolism of choline, catalyzing the oxidative demethylation of dimethylglycine to form sarcosine. The enzyme is found as a monomer in the mitochondrial matrix, and uses flavin adenine dinucleotide and folate as cofactors. Mutation in this gene causes dimethylglycine dehydrogenase deficiency, characterized by a fishlike body odor, chronic muscle fatigue, and elevated levels of the muscle form of creatine kinase in serum. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]