C10orf90 (NM_001004298) Human Tagged ORF Clone

CAT#: RC223255

C10orf90 (Myc-DDK-tagged)-Human chromosome 10 open reading frame 90 (C10orf90)

Product Images

Western blot validation of overexpression lysate (Cat# LY424076) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC223255 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: C10orf90
• Synonyms: bA422P15.2; FATS
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC223255 representing NM_001004298
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTCAAACTGTCTGGAGAAGGATTACGGGACAGCTACCACAGTAGAAGAGATCAAATTGCCCTTAAAA
  ATCTCCAGTCTGATGTCACAGAGGCAAAATCTGACTTCACCAAGGAGACCCTGGCATCACAAAACACAAA
  AATGATTTCATCCATAGTCATCTCCCAGATGATTGATGAGAATAAGTCAAGAGAAAACAGGGCCTCCTTG
  CCCCTACCGTGTGCCATTGCTCAGTCTCGTGCACATCACGCCAAGCAATCTCTGGCTAACCGCAGCGGAG
  TCAACATTCACAGAGCATTTGCGTTACTTCCGGGCAGATTAGGAATCCCGGCACCGTCAGATGAGCGAGG
  ACCTGAGGCAGAGCTGCCGCCCAAAGAGGAGAGACCCTGTGGGGGCCCCCGCAGAGGGTTTGCATCCATC
  ACCATCACGGCCAGACGCGTGGGCCCCCCAGCCCGCGCCCTGGTGTGGGGGACTGCTGGGGACTCTCTGT
  GCCCCAAGTGCAGGGCTGAGGACACACTGTTCCAGGCGCCCCCGGCTCTGGCCAATGGCGCCCATCCAGG
  TCGGCATCAGAGATCTTTTGCCTGCACAGAGTTCTCCAGAAACAGCTCCGTGGTGCGGCTGAAGGTTCCC
  GAGGCCCACACTGGGTTGTGTGAGAGACGCAAGTACTGGGTCACCCATGCAGACGACAAAGAGACCAGTT
  TTTCTCCAGACACCCCACTGTCAGGAAAGAGCCCGCTGGTGTTCAGTTCCTGTGTCCACCTCAGGGTGTC
  TCAGCAGTGTCCAGATTCAATCTATTACGTAGACAAGTCTCTCTCCGTCCCCATTGAGCCACCTCAAATT
  GCCAGCCCCAAAATGCACAGATCCGTCCTGTCGCTCAACCTCAATTGTAGTTCCCACAGATTAACAGCAG
  ATGGAGTAGATGGCCTAGTGAACAGAGAGCCAATAAGCGAAGCCCTGAAGCAGGAGCTCCTGGAGGGAGA
  CCAGGACCTCGTAGGCCAGCGCTGGAACCCAGGTTTACAAGAAAGTCACTTGAAGGAAACCCCATCGTTG
  AGGCGGGTGCATTTGGGGACCGGCGCTTGTCCTTGGAGTGGTTCTTTTCCATTGGAAAACACAGAATTGG
  CAAATGTGGGAGCTAACCAAGTCACTGTAAGAAAAGGGGAAAAGGACCATACAACTCATTGTCACGCCAG
  CGATCATGCCAACCAACTGTCCATTCACATTCCTGGCTGGAGTTACAGGGCAGTACACACAAAAGTATTT
  TCTGGAAGCAGCAAGAGGCAACAAGGAGAAGTATGTATGACTGTGTCTGCTCCTCCAGTGGAACAGAAGC
  CCACTAGACATTTCCTTCCCATTGGGGATAGCTCTCCAAGCGATGACTGTCTGTCTAGAGACCTTTCTGA
  GCCCACAGAGAGACGACATCAAAGCTTCCTGAAACCCAGAATCCTTTTTCCTGGGTTTCTTTGCCCCTTA
  CAAGATGTATGTGCATCTCTGCAGGAAGACAATGGTGTTCAGATAGAAAGCAAGTTCCCCAAAGGAGATT
  ACACATGCTGTGACTTGGTTGTAAAAATAAAGGAATGTAAGAAGAGTGAGGACCCCACCACACCTGAGCC
  CTCCCCAGCAGCACCCTCGCCAGCACCCCGCGATGGAGCAGGGAGCCCTGGCCTGTCCGAAGACTGTTCT
  GAGTCTCAGCAAACGCCTGCAAGATCTTTGACCTTGCAAGAAGCACTGGAAGTTCGTAAGCCTCAGTTCA
  TTTCTCGCTCACAAGAACGGCTGAAGAAGCTTGAACACATGGTCCAGCAGAGGAAAGCCCAGCGGAAGGA
  GGACCTGAGGCAGAAGCAGAGCCTCCTTCCCATCCGCACCAGCAAGAAGCAGTTCACGATTCCCCATCCT
  CTGAGTGATAACTTGTTCAAACCCAAAGAACGGTGCATTTCAGAGAAGGAGATGCATATGCGATCTAAGA
  GAATCTATGATAACTTGCCGGAAGTTAAAAAGAAAAAAGAAGAACAAAGGAAAAGGGTGATCTTACAAAG
  TAACAGACTCCGTGCCGAAGTCTTCAAAAAGCAATTACTGGACCAGCTCCTTCAAAGGAATGCGGTC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC223255 representing NM_001004298
  Red=Cloning site Green=Tags(s)
  
  MLKLSGEGLRDSYHSRRDQIALKNLQSDVTEAKSDFTKETLASQNTKMISSIVISQMIDENKSRENRASL
  PLPCAIAQSRAHHAKQSLANRSGVNIHRAFALLPGRLGIPAPSDERGPEAELPPKEERPCGGPRRGFASI
  TITARRVGPPARALVWGTAGDSLCPKCRAEDTLFQAPPALANGAHPGRHQRSFACTEFSRNSSVVRLKVP
  EAHTGLCERRKYWVTHADDKETSFSPDTPLSGKSPLVFSSCVHLRVSQQCPDSIYYVDKSLSVPIEPPQI
  ASPKMHRSVLSLNLNCSSHRLTADGVDGLVNREPISEALKQELLEGDQDLVGQRWNPGLQESHLKETPSL
  RRVHLGTGACPWSGSFPLENTELANVGANQVTVRKGEKDHTTHCHASDHANQLSIHIPGWSYRAVHTKVF
  SGSSKRQQGEVCMTVSAPPVEQKPTRHFLPIGDSSPSDDCLSRDLSEPTERRHQSFLKPRILFPGFLCPL
  QDVCASLQEDNGVQIESKFPKGDYTCCDLVVKIKECKKSEDPTTPEPSPAAPSPAPRDGAGSPGLSEDCS
  ESQQTPARSLTLQEALEVRKPQFISRSQERLKKLEHMVQQRKAQRKEDLRQKQSLLPIRTSKKQFTIPHP
  LSDNLFKPKERCISEKEMHMRSKRIYDNLPEVKKKKEEQRKRVILQSNRLRAEVFKKQLLDQLLQRNAV
  
  SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001004298
• ORF Size: 2097 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001004298.2, NP_001004298.2
• RefSeq Size: 3097 bp
• RefSeq ORF: 2100 bp
• Locus ID: 118611
• Cytogenetics: 10q26.2
• MW: 77.7 kDa