CCR5 (NM_000579) Human Tagged Lenti ORF Clone
CAT#: RC223291L2
- LentiORF®
Lenti-ORF clone of CCR5 (mGFP-tagged)-Human chemokine (C-C motif) receptor 5 (CCR5), transcript variant A
"NM_000579" in other vectors (6)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | mGFP |
Symbol | CCR5 |
Synonyms | CC-CKR-5; CCCKR5; CCR-5; CD195; CKR-5; CKR5; CMKBR5; IDDM22 |
Vector | pLenti-C-mGFP |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
The ORF insert of this clone is exactly the same as(RC223291).
|
Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
ACCN | NM_000579 |
ORF Size | 1056 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_000579.3, NP_000570.1 |
RefSeq Size | 3655 bp |
RefSeq ORF | 1059 bp |
Locus ID | 1234 |
UniProt ID | P51681 |
Cytogenetics | 3p21.31 |
Protein Families | Druggable Genome, ES Cell Differentiation/IPS, GPCR, Transmembrane |
Protein Pathways | Chemokine signaling pathway, Cytokine-cytokine receptor interaction, Endocytosis |
MW | 40.3 kDa |
Gene Summary | This gene encodes a member of the beta chemokine receptor family, which is predicted to be a seven transmembrane protein similar to G protein-coupled receptors. This protein is expressed by T cells and macrophages, and is known to be an important co-receptor for macrophage-tropic virus, including HIV, to enter host cells. Defective alleles of this gene have been associated with the HIV infection resistance. The ligands of this receptor include monocyte chemoattractant protein 2 (MCP-2), macrophage inflammatory protein 1 alpha (MIP-1 alpha), macrophage inflammatory protein 1 beta (MIP-1 beta) and regulated on activation normal T expressed and secreted protein (RANTES). Expression of this gene was also detected in a promyeloblastic cell line, suggesting that this protein may play a role in granulocyte lineage proliferation and differentiation. This gene is located at the chemokine receptor gene cluster region. An allelic polymorphism in this gene results in both functional and non-functional alleles; the reference genome represents the functional allele. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2015] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC110858 | CCR5 (untagged)-Human chemokine (C-C motif) receptor 5 (CCR5), transcript variant A |
USD 310.00 |
|
RC223291 | CCR5 (Myc-DDK-tagged)-Human chemokine (C-C motif) receptor 5 (CCR5), transcript variant A |
USD 420.00 |
|
RG223291 | CCR5 (GFP-tagged) - Human chemokine (C-C motif) receptor 5 (CCR5), transcript variant A |
USD 460.00 |
|
RC223291L1 | Lenti-ORF clone of CCR5 (Myc-DDK-tagged)-Human chemokine (C-C motif) receptor 5 (CCR5), transcript variant A |
USD 620.00 |
|
RC223291L3 | Lenti-ORF clone of CCR5 (Myc-DDK-tagged)-Human chemokine (C-C motif) receptor 5 (CCR5), transcript variant A |
USD 768.00 |
|
RC223291L4 | Lenti-ORF clone of CCR5 (mGFP-tagged)-Human chemokine (C-C motif) receptor 5 (CCR5), transcript variant A |
USD 620.00 |
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