CCR5 (NM_000579) Human Untagged Clone

CAT#: SC110858

CCR5 (untagged)-Human chemokine (C-C motif) receptor 5 (CCR5), transcript variant A

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CCR5
• Synonyms: CC-CKR-5; CCCKR5; CCR-5; CD195; CKR-5; CKR5; CMKBR5; IDDM22
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_000579, RT-PCR generated
  ATGGATTATCAAGTGTCAAGTCCAATCTATGACATCAATTATTATACATCGGAGCCCTGC
  CAAAAAATCAATGTGAAGCAAATCGCAGCCCGCCTCCTGCCTCCGCTCTACTCACTGGTG
  TTCATCTTTGGTTTTGTGGGCAACATGCTGGTCATCCTCATCCTGATAAACTGCAAAAGG
  CTGAAGAGCATGACTGACATCTACCTGCTCAACCTGGCCATCTCTGACCTGTTTTTCCTT
  CTTACTGTCCCCTTCTGGGCTCACTATGCTGCCGCCCAGTGGGACTTTGGAAATACAATG
  TGTCAACTCTTGACAGGGCTCTATTTTATAGGCTTCTTCTCTGGAATCTTCTTCATCATC
  CTCCTGACAATCGATAGGTACCTGGCTGTCGTCCATGCTGTGTTTGCTTTAAAAGCCAGG
  ACGGTCACCTTTGGGGTGGTGACAAGTGTGATCACTTGGGTGGTGGCTGTGTTTGCGTCT
  CTCCCAGGAATCATCTTTACCAGATCTCAAAAAGAAGGTCTTCATTACACCTGCAGCTCT
  CATTTTCCATACAGTCAGTATCAATTCTGGAAGAATTTCCAGACATTAAAGATAGTCATC
  TTGGGGCTGGTCCTGCCGCTGCTTGTCATGGTCATCTGCTACTCGGGAATCCTAAAAACT
  CTGCTTCGGTGTCGAAATGAGAAGAAGAGGCACAGGGCTGTGAGGCTTATCTTCACCATC
  ATGATTGTTTATTTTCTCTTCTGGGCTCCCTACAACATTGTCCTTCTCCTGAACACCTTC
  CAGGAATTCTTTGGCCTGAATAATTGCAGTAGCTCTAACAGGTTGGACCAAGCTATGCAG
  GTGACAGAGACTCTTGGGATGACGCACTGCTGCATCAACCCCATCATCTATGCCTTTGTC
  GGGGAGAAGTTCAGAAACTACCTCTTAGTCTTCTTCCAAAAGCACATTGCCAAACGCTTC
  TGCAAATGCTGTTCTATTTTCCAGCAAGAGGCTCCCGAGCGAGCAAGCTCAGTTTACACC
  CGATCCACTGGGGAGCAGGAAATATCTGTGGGCTTGTGA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000579 unedited
  TTGGAACCCAAGTACGGTATTTGTTACACNATACACTATAGGCGGCCGCGCAATCANATC
  TGGTACCGAGCTCGGCTCCACTAGTAACGGCCGCCAGTGTGCTGGAATTCGGCTTGATCG
  GAACAAGATGGATTATCAAGTGTCAAGTCCAATCTATGACATCAATTATTATACATCGGA
  GCCCTGCCAAAAAATAATGTGAAGCANATCGCAGCCCGTCTTTTTCTCCGCTCTACTCAC
  TGGTGATCATCTTTGGTTTTGAGGGCAACAAGCTGGGCCAACCCCATTCCGAAAAACCGC
  CAAAGGGTTTAAAAAACAGGGTTTGATTTTAACGGGGTAAACCGGGGAAATTTTTAAGCG
  GGGTTTTTTTTTTTTAAAAGCCCCCCTTTTGGGGGGAAAAAATATTTTTCCCCCCCCCCC
  GGGGGGTTTGGTTAAAAAAAAAAAAAAAAGGGTTTTTTTTTGTGGGGGGGGGGGGGGGAN
  AATAAAAAAGGAAGGGGGGGGGGGGGGGGGGGGAAAAAAAAAAATAATGACCCTTTCCCC
  TCCCAAAAAAAAAAAAAAAAAAGAAAAAAAAGAATACGCTCGTGGGGGGTGGGGATAGAA
  TGNGNAGAAAGAAAAAAAAAAAGCACCCCCCCACAACTCTTATACAACTATTGTGTGCGG
  GTGAAAGAAAAAAAAAAAAATATATTTCGCGGGGGGGGGGGGTTAGGTTNTTTATTTTTT
  TTANNNTNTNNNCCTTTCTAAAGGCCCCCTCTTCTGTCCAATTATAGAGATAAGAAGTAA
  GAGGGTGAGGGGAGCGGCGAGGGTCCTGCTCGCCTCCCTACACCTCTGATTTTCCCGCGG
  GGCGAAAGAAAACAAAACGACAATAAAAAAAAAAAAAAAC
  
• Restriction Sites: Please inquire
• ACCN: NM_000579
• Insert Size: 3700 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000579.1, NP_000570.1
• RefSeq Size: 3655 bp
• RefSeq ORF: 1059 bp
• Locus ID: 1234
• Cytogenetics: 3p21.31
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, GPCR, Transmembrane
• Protein Pathways: Chemokine signaling pathway, Cytokine-cytokine receptor interaction, Endocytosis
• Gene Summary: 'This gene encodes a member of the beta chemokine receptor family, which is predicted to be a seven transmembrane protein similar to G protein-coupled receptors. This protein is expressed by T cells and macrophages, and is known to be an important co-receptor for macrophage-tropic virus, including HIV, to enter host cells. Defective alleles of this gene have been associated with the HIV infection resistance. The ligands of this receptor include monocyte chemoattractant protein 2 (MCP-2), macrophage inflammatory protein 1 alpha (MIP-1 alpha), macrophage inflammatory protein 1 beta (MIP-1 beta) and regulated on activation normal T expressed and secreted protein (RANTES). Expression of this gene was also detected in a promyeloblastic cell line, suggesting that this protein may play a role in granulocyte lineage proliferation and differentiation. This gene is located at the chemokine receptor gene cluster region. An allelic polymorphism in this gene results in both functional and non-functional alleles; the reference genome represents the functional allele. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2015]'
  Transcript Variant: This variant (A) represents the longer transcript. Both variants encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by published experimental evidence.