ketohexokinase (KHK) (NM_006488) Human Tagged ORF Clone

CAT#: RC223488

KHK (Myc-DDK-tagged)-Human ketohexokinase (fructokinase) (KHK), transcript variant b

Product Images

Western blot validation of overexpression lysate (Cat# LY416608) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC223488 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified KHK protein (Cat# TP323488). The protein was produced from HEK293T cells transfected with KHK cDNA clone (Cat# RC223488) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: KHK
• Synonyms: ketohexokinase; ketohexokinase (fructokinase)
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC223488 representing NM_006488
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAGAGAAGCAGATCCTGTGCGTGGGGCTAGTGGTGCTGGACGTCATCAGCCTGGTGGACAAGTACC
  CTAAGGAGGACTCGGAGATAAGGTGTTTGTCCCAGAGATGGCAGCGCGGAGGCAACGCGTCCAACTCCTG
  CACCGTTCTCTCCCTGCTCGGAGCCCCCTGTGCCTTCATGGGCTCAATGGCTCCTGGCCATGTTGCTGAC
  TTCCTGGTGGCCGACTTCAGGCGGCGGGGCGTGGACGTGTCTCAGGTGGCCTGGCAGAGCAAGGGGGACA
  CCCCCAGCTCCTGCTGCATCATCAACAACTCCAATGGCAACCGTACCATTGTGCTCCATGACACGAGCCT
  GCCAGATGTGTCTGCTACAGACTTTGAGAAGGTTGATCTGACCCAGTTCAAGTGGATCCACATTGAGGGC
  CGGAACGCATCGGAGCAGGTGAAGATGCTGCAGCGGATAGACGCACACAACACCAGGCAGCCTCCAGAGC
  AGAAGATCCGGGTGTCCGTGGAGGTGGAGAAGCCACGAGAGGAGCTCTTCCAGCTGTTTGGCTACGGAGA
  CGTGGTGTTTGTCAGCAAAGATGTGGCCAAGCACTTGGGGTTCCAGTCAGCAGAGGAAGCCTTGAGGGGC
  TTGTATGGTCGTGTGAGGAAAGGGGCTGTGCTTGTCTGTGCCTGGGCTGAGGAGGGCGCCGACGCCCTGG
  GCCCTGATGGCAAATTGCTCCACTCGGATGCTTTCCCGCCACCCCGCGTGGTGGATACACTGGGAGCTGG
  AGACACCTTCAATGCCTCCGTCATCTTCAGCCTCTCCCAGGGGAGGAGCGTGCAGGAAGCACTGAGATTC
  GGGTGCCAGGTGGCCGGCAAGAAGTGTGGCCTGCAGGGCTTTGATGGCATCGTG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC223488 representing NM_006488
  Red=Cloning site Green=Tags(s)
  
  MEEKQILCVGLVVLDVISLVDKYPKEDSEIRCLSQRWQRGGNASNSCTVLSLLGAPCAFMGSMAPGHVAD
  FLVADFRRRGVDVSQVAWQSKGDTPSSCCIINNSNGNRTIVLHDTSLPDVSATDFEKVDLTQFKWIHIEG
  RNASEQVKMLQRIDAHNTRQPPEQKIRVSVEVEKPREELFQLFGYGDVVFVSKDVAKHLGFQSAEEALRG
  LYGRVRKGAVLVCAWAEEGADALGPDGKLLHSDAFPPPRVVDTLGAGDTFNASVIFSLSQGRSVQEALRF
  GCQVAGKKCGLQGFDGIV
  
  SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006488
• ORF Size: 894 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006488.1, NM_006488.2, NP_006479.1
• RefSeq Size: 1899 bp
• RefSeq ORF: 897 bp
• Locus ID: 3795
• Cytogenetics: 2p23.3
• Domains: pfkB
• Protein Families: Druggable Genome
• Protein Pathways: Fructose and mannose metabolism, Metabolic pathways
• MW: 32.3 kDa
• Gene Summary: 'This gene encodes ketohexokinase that catalyzes conversion of fructose to fructose-1-phosphate. The product of this gene is the first enzyme with a specialized pathway that catabolizes dietary fructose. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]'