ketohexokinase (KHK) (NM_006488) Human Untagged Clone

CAT#: SC124910

KHK (untagged)-Human ketohexokinase (fructokinase) (KHK), transcript variant b

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: KHK
• Synonyms: ketohexokinase; ketohexokinase (fructokinase)
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC124910 sequence for NM_006488 edited (data generated by NextGen Sequencing)
  ATGGAAGAGAAGCAGATCCTGTGCGTGGGGCTAGTGGTGCTGGACGTCATCAGCCTGGTG
  GACAAGTACCCTAAGGAGGACTCGGAGATAAGGTGTTTGTCCCAGAGATGGCAGCGCGGA
  GGCAACGCGTCCAACTCCTGCACCATTCTCTCCCTGCTCGGAGCCCCCTGTGCCTTCATG
  GGCTCAATGGCTCCTGGCCATGTTGCTGACTTCCTGGTGGCCGACTTCAGGCGGCGGGGC
  GTGGACGTGTCTCAGGTGGCCTGGCAGAGCAAGGGGGACACCCCCAGCTCCTGCTGCATC
  ATCAACAACTCCAATGGCAACCGTACCATTGTGCTCCATGACACGAGCCTGCCAGATGTG
  TCTGCTACAGACTTTGAGAAGGTTGATCTGACCCAGTTCAAGTGGATCCACATTGAGGGC
  CGGAACGCATCGGAGCAGGTGAAGATGCTGCAGCGGATAGACGCACACAACACCAGGCAG
  CCTCCAGAGCAGAAGATCCGGGTGTCCGTGGAGGTGGAGAAGCCACGAGAGGAGCTCTTC
  CAGCTGTTTGGCTACGGAGACGTGGTGTTTGTCAGCAAAGATGTGGCCAAGCACTTGGGG
  TTCCAGTCAGCAGAGGAAGCCTTGAGGGGCTTGTATGGTCGTGTGAGGAAAGGGGCTGTG
  CTTGTCTGTGCCTGGGCTGAGGAGGGCGCCGACGCCCTGGGCCCTGATGGCAAATTGCTC
  CACTCGGATGCTTTCCCGCCACCCCGCGTGGTGGATACACTGGGAGCTGGAGACACCTTC
  AATGCCTCCGTCATCTTCAGCCTCTCCCAGGGGAGGAGCGTGCAGGAAGCACTGAGATTC
  GGGTGCCAGGTGGCCGGCAAGAAGTGTGGCCTGCAGGGCTTTGATGGCATCGTGTGA
  
  Clone variation with respect to NM_006488.2
  145 g=>a
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006488 unedited
  AGCATTTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACCAGGCTCTGGGN
  NAGTAGCCTCATGGNAAGAGAAGCAGATCCTGTGCGTGGGGCTAGTGGTGCTGGACGTCA
  TCAGCCTGGTGGACAAGTACCCTAAGGAGGACTCGGAGATAAGGTGTTTGTCCCAGAGAT
  GGCAGCGCGGAGGCAACGCGTCCAACTCCTGCACCATTCTCTCCCTGCTCGGAGCCCCCT
  GTGCCTTCATGGGCTCAATGGCTCCTGGCCATGTTGCTGACTTCCTGGTGGCCGACTTCA
  GGCGGCGGGGCGTGGACGTGTCTCAGGTGGCCTGGCAGAGCAAGGGGGACACCCCCAGCT
  CCTGCTGCATCATCAACAACTCCAATGGCAACCGTACCATTGTGCTCCATGACACGAGCC
  TGCCAGATGTGTCTGCTACAGACTTTGAGAAGGTTGATCTGACCCAGTTCAAGTGGATCC
  ACATTGAGGGCCGGAACGCATCGGAGCAGGTGAAGATGCTGCAGCGGATAGACGCACACA
  ACACCAGGCAGCCTCCAGAGCAGAAGATCCGGGTGTCCGTGGAGGTGGAGAAGCCACGAG
  AGGAGCTCTTCCAGCTGTTTGGCTACGGAGACGTGGTGTTTGTCAGCAAAGATGTGGCCA
  AGCACTTGGGGTTCCAGTCAGCAGAGGAAGCCTTGAGGGGCTTGTATGGTCGTGTGAGGA
  AAGGGGCTGTGCTTGTCTGTGCCTGGGCTGAGAGGGCGCCGACGCCCTGNGCCCCTGATG
  GCAATTGCTCCACTCGGATGCTTTTCCGCCACCCCGCGTGGTGGATACACTGGGAGCTGG
  AGACACCTTCATGCCTNCGTCATCTTCAGCCTCTCCCAGGGGGAGAGCGTGCAGAAGCAC
  TGAGATTCGGTGCCCAGTGCCCGCAGAAATGTGGCTGCAGGCTTGATGCATCTGTGAAAC
  AGTGCCGCTCTACAACATGGAACACATGGGCTGCTGGCTTTCCTC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_006488 unedited
  TATGGACGCGGCCGCATTCTANAGTCGGTTTTTTTTTTTTTTTTTTTGGCTCTGAGGAAG
  ATTATTTGCTCTGAGGCTCTGCATCCCCCAGCCATCCCCCCAAAGCCTCTCCCTGTGGGG
  AACACAGGACACAGGCAGAGTCCTCCCCACAGCTTGCATCTGTCCCCTGAACAGGGCAAC
  CTGGACGCCAGGCTGGATGGAGGGGAGAAGGCGATGCAGCCGCAATGGTAGTCTCCATGG
  TGTGTGAGGAGCCGGCACCTGCTCTCACACGATGCCATCAAAGCCCTGCAGGCCACACTT
  CTTGCCGGCCACCTGGCACCCGAATCTCAGTGCTTCCTGCACGCTCCTCCCCTGGGAGAG
  GCTGAAGATGACGGAGGCATTGAAGGTGTCTCCAGCTCCCAGTGTATCCACCACGCGGGG
  TGGCGGGAAAGCATCCGAGTGGAGCAATTTGCCATCAGGGCCCAGGGCGTCGGCGCCCTC
  CTCAGCCCAGGCACAGACAAGCACAGCCCCTTTCCTCACACGACCATACAAGCCCCTCAA
  GGCTTCCTCTGCTGACTGGAACCCCAAGTGCTTGGCCACATCTTTGCTGACAAACACCAC
  GTCTCCGTAGCCAAACAGCTGGAAGAGCTCCTCTCGTGGCTTCTCCACCTCCACGGACAC
  CCGGATCTTCTGCTCTGGAGGCTGCCTGGTGTTGTGTGCGTCTATCCGCTGCAGCATCTT
  CACCTGCTCCGATGCGTTCCGGCCCTCAATGTGGATCCACTTGAACTGGGTCAGATCAAC
  CTTCTCAAAGTCTGTAGCAGACACATCTGGCAGGCTCGTGTCATGGAGCACAATGGTACG
  GTTGCCATTGGAGTTGTTGATGATGCANCANGAGCTGGGGGGTGTCCCCCTTGCTCTGCC
  NAGGCCACCTGAGAACGTNCACGCCCCGCCGCNNCTGAGTCGNCACCAGNAAAGTCAGCA
  CATGGCCCAGGAGCCATTGAG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_006488
• Insert Size: 1000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006488.1, NP_006479.1
• RefSeq Size: 1899 bp
• RefSeq ORF: 897 bp
• Locus ID: 3795
• Cytogenetics: 2p23.3
• Domains: pfkB
• Protein Families: Druggable Genome
• Protein Pathways: Fructose and mannose metabolism, Metabolic pathways
• Gene Summary: 'This gene encodes ketohexokinase that catalyzes conversion of fructose to fructose-1-phosphate. The product of this gene is the first enzyme with a specialized pathway that catabolizes dietary fructose. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant utilizes the alternate exon 3c and encodes isoform b, which exhibits higher enzymatic activity than isoform a.